Polymer probes and methods

Patent metadata

Field	Value
Publication number	US-10788477-B2
Application number	US-201615332588-A
Country	US
Kind code	B2
Filing date	Oct 24, 2016
Priority date	Oct 26, 2015
Publication date	Sep 29, 2020
Grant date	Sep 29, 2020

Abstract

A polymer detection probe is provided that includes a binding module that specifically binds to at least one polymer and a reporter module that is spectroscopically detectable. The binding module can be a carbohydrate-binding module (CBM). The reporter module can be a fluorescent protein. A complex is provided that includes a probe specifically bound to a pulp or paper product including at least one surface available lignocellulosic polymer. A pulp or paper product is provided that includes at least one surface available lignocellulosic polymer and at least one probe bound thereto. Methods are provided that employ a lignocellulosic probe. A method of detecting a lignocellulosic polymer or other type of polymer is provided. A method of determining the effectiveness of an industrial treatment on pulp or a paper product is also provided. A method of determining a physical property of pulp or a paper product is further provided.

First claim

What is claimed is:

1. A method of detecting a lignocellulosic polymer, the method comprising:
contacting a lignocellulosic polymer detection probe with a biomass material, said lignocellulosic polymer detection probe comprising a) a binding module that specifically binds to at least one lignocellulosic polymer and b) a reporter module that is spectroscopically detectable, wherein the binding module is a binding module polypeptide that comprises the amino acid sequence of SEQ ID NO: 14, 16, 18, 20, or 22, and wherein the reporter module is a reporter module polypeptide that comprises the amino acid sequence of SEQ ID NO: 24, 26, 28, or 30; and
measuring a property associated with the reporter module to determine the presence or absence of at least one lignocellulosic polymer in the biomass material based on specific binding of the probe to the at least one lignocellulosic polymer.

2. The method of claim 1 , wherein the property measured is fluorescence.

3. The method of claim 1 , further comprising calculating the amount of the at least one lignocellulosic polymer, determining the type of the at least one lignocellulosic polymer, or both.

4. The method of claim 1 , wherein the biomass material comprises a wood biomass material.

5. The method of claim 4 , wherein the wood biomass material pulp, furnish, paper, or any combination thereof.

6. The method of claim 5 , further comprising forming at least one handsheet from the wood biomass product, wherein the measuring is performed on the handsheet.

7. The method of claim 1 , wherein the measuring is performed before treatment, during treatment, or after treatment, or any combination thereof.

8. The method of claim 7 , wherein the treatment comprises an enzymatic treatment, bleaching, amorphogenesis, milling, or PFI refining, or any combination thereof.

9. The method of claim 7 , wherein the treatment comprises enzymatic treatment with at least one enzyme comprising a cellulase, a xylanase, a mannase, a lignase, or any combination thereof.

10. The method of claim 3 , further comprising performing at least one treatment of the biomass material based on the amount of the at least one lignocellulosic polymer measured, the type of lignocellulosic polymer measured, or both.

11. The method of claim 3 , wherein the amount of lignocellulosic polymer measured correlates negatively or positively with at least one physical property of the biomass material.

12. The method of claim 11 , wherein the at least one physical property comprises burst index, drainage rate, tear index, tensile index, or internal bond strength, or any combination thereof.

13. The method of claim 3 , further comprising dosing at least one enzyme based on the amount of lignocellulosic polymer measured, the type of lignocellulosic polymer measured, or both.

14. The method of claim 3 , further comprising adjusting mill speed based on the amount of lignocellulosic polymer measured, the type of lignocellulosic polymer measured, or both.

15. The method of claim 3 , further comprising adjusting total water content of the biomass material based on the amount of lignocellulosic polymer measured, the type of lignocellulosic polymer measured, or both.

16. The method of claim 1 , wherein the probe comprises a plurality of probes.

17. A method of determining the effectiveness of an industrial treatment on pulp or a paper product comprising:
contacting a lignocellulosic polymer detection probe with a pulp or a paper product, said lignocellulosic polymer detection probe comprising a) a binding module that specifically binds to at least one lignocellulosic polymer and b) a reporter module that is spectroscopically detectable, wherein the binding module is a binding module polypeptide that comprises the amino acid sequence of SEQ ID NO: 14, 16, 18, 20, or 22, and wherein the reporter module is a reporter module polypeptide that comprises the amino acid sequence of SEQ ID NO: 24, 26, 28, or 30;
detecting the specific binding of the probe to the pulp or the paper product;
calculating the amount of at least one lignocellulosic polymer on a surface of the pulp or the paper product; and
determining the effectiveness of an industrial treatment on the pulp or paper product based on the amount of the at least one lignocellulosic polymer detected.

18. The method of claim 17 , wherein the industrial treatment comprises an enzymatic treatment, a chemical treatment, or a physical treatment, or any combination thereof.

19. The method of claim 17 , wherein the method is performed before the industrial treatment, during the industrial treatment, or after the industrial treatment, or any combination thereof.

20. The method of claim 17 , wherein the lignocellulosic polymer detection probe is detectable at a distinct wavelength.

21. A method of determining a physical property of pulp or a paper product comprising:
contacting a lignocellulosic polymer detection probe with a pulp or a paper product, said lignocellulosic polymer detection probe comprising a) a binding module that specifically binds to at least one lignocellulosic polymer and b) a reporter module that is spectroscopically detectable, wherein the binding module is a binding module polypeptide that comprises the amino acid sequence of SEQ ID NO: 14, 16, 18, 20, or 22, and wherein the reporter module is a reporter module polypeptide that comprises the amino acid sequence of SEQ ID NO: 24, 26, 28, or 30;
detecting the specific binding of the probe to the pulp or the paper product;
calculating the amount of at least one lignocellulosic polymer on a surface of the pulp or the paper product; and
determining at least one physical property of the pulp or paper product based on the amount of the at least one lignocellulosic polymer detected.

22. The method of claim 21 , wherein the at least one physical property comprises burst index, drainage rate, tear index, tensile index, or internal bond strength, or any combination thereof.

23. A method of detecting a polymer, the method comprising:
contacting a polymer detection probe with a material, said polymer detection probe comprising a) a binding module that specifically binds to at least one polymer and b) a reporter module that is spectroscopically detectable, wherein the binding module is a binding module polypeptide polypeptide that comprises the amino acid sequence of SEQ ID NO: 14, 16, 18, 20, or 22, and wherein the reporter module is a reporter module polypeptide that comprises the amino acid sequence of SEQ ID NO: 24, 26, 28, or 30; and
measuring a property associated with the reporter module to determine the presence or absence of at least one polymer in the material based on specific binding of the probe to the at least one polymer.

24. The method of claim 23 , wherein the property measured is fluorescence.

25. The method of claim 23 , further comprising calculating the amount of the at least one polymer, determining the type of the at least one polymer, or both.

26. The method of claim 23 , wherein the material comprises a blood sample.

27. The method of 26 , further comprising determining at least one of a blood antigen, type, group, and subgroup of the blood sample.

28. The method of claim 23 , wherein the probe comprises a plurality of probes.

29. The method of claim 1 , wherein the binding module polypeptide is fused directly to the reporter modul

Assignees

• Buckman Laboratories Int Inc
• Univ Du Quebec A Trois Rivieres

Inventors

• Beauregard Marc
• Meddeb-Mouelhi Fatma
• Hebert-Ouellet Yannick
• Janse Bernard
• Macdonald Kevin J

Classifications

• D21H23/76

  characterised by choice of auxiliary compounds which are added separately from at least one other compound, e.g. to improve the incorporation of the latter or to obtain an enhanced combined effect (D21H17/18, D21H17/70, D21H23/10 take precedence) · CPC title

• G01N33/5308

  for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites · CPC title

• G01N2400/26

  Cellulose · CPC title

• G01N33/343 Primary

  Paper pulp · CPC title

• C07K14/001

  by chemical synthesis · CPC title