Method for determination of microorganisms

Patent metadata

Field	Value
Publication number	US-10017806-B2
Application number	US-201515524718-A
Country	US
Kind code	B2
Filing date	Oct 27, 2015
Priority date	Nov 7, 2014
Publication date	Jul 10, 2018
Grant date	Jul 10, 2018

Abstract

The present invention relates to determination of the microorganism content in material comprising cellulose within the pulp and paper industry. The material comprising cellulose is enzymatically pretreated and microorganisms are determined using PCR based technology.

First claim

The invention claimed is:

1. A method for determination of a microorganism content in a material comprising cellulose within the pulp and paper industry comprising the steps of:
(a) providing a suspension of a sample of the material comprising cellulose; and
(b) treating the suspension with one or more enzyme preparation having cellulase activity; and
(c) separating the microorganisms from the enzyme treated suspension;
(d) isolating nucleic acids from the separated microorganisms; and
(e) conducting a PCR analysis on the isolated nucleic acids,
wherein the PCR result indicates the microorganism content.

2. The method according to claim 1 , wherein the suspension is provided by suspending a sample in a solution.

3. The method according to claim 1 , wherein the cellulase activity is a mixture of cellulase activities.

4. The method according to claim 1 , wherein the cellulase activity is at least one endoglucanase activity.

5. The method according to claim 1 , wherein the enzyme preparation further contains hemicellulase activity or activities.

6. The method according to claim 1 , wherein the microorganisms are separated from the suspension using filter bag centrifugation.

7. The method according to claim 1 , wherein the primers for PCR are broad-range primers for bacteria or other microorganism.

8. The method according to claim 1 , wherein the primers for PCR are specific to certain species or genus of microorganism.

9. The method according to claim 1 , wherein material comprising cellulose is consumer board.

10. The method according to claim 1 , wherein the microorganism belongs to bacteria.

11. The method according to claim 10 , wherein said bacteria represents a Bacillus species.

12. The method according to claim 1 wherein the microorganism is a fungi or mold.

13. The method according to claim 1 wherein the cells are treated with propidium monoazide.

14. The method according to claim 9 , wherein the consumer board material is board for the food industry.

Assignees

• Stora Enso Oyj

Inventors

• Partti-Pellinen Kirsi
• Räsänen Jari
• Härmälä Kielo
• Kettunen Anu
• Riihinen Kalle-Juhani

Classifications

• G01N33/343

  Paper pulp · CPC title

• G01N33/34

  Paper · CPC title

• D21H17/005

  Microorganisms or enzymes · CPC title

• D21C5/005

  Treatment of cellulose-containing material with microorganisms or enzymes · CPC title

• D06M2101/06

  cellulosic · CPC title