NMR multi-dimensional method for measuring coupling constants within several coupling networks

Patent metadata

Field	Value
Publication number	US-10782255-B2
Application number	US-201816315084-A
Country	US
Kind code	B2
Filing date	Jan 8, 2018
Priority date	Jan 13, 2017
Publication date	Sep 22, 2020
Grant date	Sep 22, 2020

Abstract

This invention provides a NMR multi-dimensional method for measuring coupling constants within several coupling networks. At first, a 90° hard pulse was performed to flip the magnetization from the Z axis to the XY plane. After t1/2, a selective 180° pulse is implemented with a simultaneous Z-direction gradient, thus reversing different protons at different slices. Then the PSYCHE element is implemented. After another t1/2, the gradient G1 and Gp are implemented. At last, the EPSI readout is used to simultaneously record both the chemical-shift and the spatial information. As a result, from different specific slices, we can extract the scalar couplings between the proton reversed at this slice and other protons. These couplings lead to splittings in the indirect dimension, from which relevant coupling constants can be measured.

First claim

The invention claimed is:

1. A nuclear magnetic resonance (NMR) multi-dimensional method for measuring coupling constants within several coupling networks, including:
1) acquiring a conventional NMR one-dimensional (1D) spectrum of a sample;
2) measuring a duration of a 90° hard pulse;
3) setting a range of the 1D spectrum for analysis;
4) setting a duration of a selective 180° pulse according to an interval of the closest resonances, then measuring the power of the selective 180° pulse, and placing the excitation center of the selective 180° pulse to the center frequency of the range of the 1D spectrum for analysis;
5) setting a spatial frequency encoding gradient (G 1 ), which satisfies γ*G 1 *L>SW 1D , where γ is the gyromagnetic ratio, L is the length of the detection region of the sample, and SW 1D is the spectral width of the 1D spectrum;
6) setting a spoiling gradient (G s );
7) setting a gradient (G 2 ), which satisfies γ*G 2 *L>SW 1D ;
8) setting a sweep range of chirp pulses (pbw), which satisfies pbw>γ*G 2 *L;
9) setting a flip angle (β) and a duration of the chirp pulses, and measuring the power of the chirp pulses with the flip angle (β), wherein sweeping directions of the chirp pulses are opposite;
10) setting a gradient (G a ), which satisfies γ*G a *L<SW, where SW is a sampling frequency and γ*G a *L>>SW 1D ;
11) setting a gradient (G p ), which is used to adjust positions of echo centers;
12) setting a duration of an acquisition window (T a ), which satisfies 1/(2*T a )≥SW 1D ;
13) setting a number of increments in the indirect dimension (ni) and a number of loops of the acquisition windows (N); and
14) using the measured 90° hard pulse as excitation pulses of a pulse sequence, after t 1 /2, implementing the selective 180° pulse and the gradient(G 1 ), then implementing a PSYCHE element, and after another t 1 /2, implementing the gradient (G 1 ) and the gradient (G p ), then repeating an EPSI readout for N times and acquiring NMR signals, wherein:
the PSYCHE element includes two chirp pulses with small flip angle and opposite sweeping directions, and a simultaneously applied instance of the gradient (G 2 ),
the PSYCHE element is flanked by two of the spoiling gradients (G s ), and
the EPSI readout includes the gradient (G a ) and gradient (−G a ), and simultaneous samplings, of which a duration under one gradient is the duration (T a ).

2. An NMR multi-dimensional method for measuring coupling constants within several coupling networks according to claim 1 , wherein:
the number of the increments (ni) is set according to required digital resolution in the indirect dimension: ni=SW 1 /v 1 , where v 1 is the required digital resolution in the indirect dimension and SW 1 is the spectral width in the indirect dimension.

3. An NMR multi-dimensional method for measuring coupling constants within several coupling networks according to claim 1 , wherein:
the number of loops of the acquisition windows (N) is set according to a required digital resolution in the direct dimension: N=SW 1D /v 2 , where v 2 is the required digital resolution in the direct dimension.

4. An NMR multi-dimensional method for measuring coupling constants within several coupling networks according to claim 1 , wherein:
the flip angle of the chirp pulses (β) is chosen for a balance between signal-to-noise ratio (SNR) and spectral purity, wherein a larger flip angle (β) results in higher SNR, but leads to stronger artifact, and the flip angle (β) is set to 15°˜20°.

5. A nuclear magnetic resonance (NMR) multi-dimensional method for measuring coupling constants within several coupling networks, including:
1) acquiring a conventional NMR one-dimensional (1D) spectrum of a sample;
2) measuring a duration of a 90° hard pulse;
3) setting a range of the 1D spectrum for analysis;
4) setting a duration of a selective 180° pulse according to an interval of the closest resonances, then measuring the power of the selective 180° pulse, and placing the excitation center of the selective 180° pulse to the center frequency of the range of the 1D spectrum for analysis;
5) setting a spatial frequency encoding gradient (G 1 ), which satisfies γ*G 1 *L>SW 1D , where γ is the gyromagnetic ratio, L is the length of the detection region of the sample, and SW 1D is the spectral width of the 1D spectrum;
6) setting a spoiling gradient (G s );
7) setting a gradient (G 2 ), which satisfies γ*G 2 *L>SW 1D ;
8) setting a sweep range of chirp pulses (pbw), which satisfies pbw>γ*G 2 *L;
9) setting a flip angle (β) and a duration of the chirp pulses, and measuring the power of the chirp pulses with the flip angle (β), wherein sweeping directions of the chirp pulses are opposite;
10) setting a gradient (G a ), which satisfies γ*G a *L<SW, where SW is a sampling frequency and γ*G a *L>>SW 1D ;
11) setting a gradient (G p ), which is used to adjust positions of echo centers;
12) setting a duration of an acquisition window (T a ), which satisfies 1/(2*T a )≥SW 1D ;
13) setting a number of increments in the indirect dimension (ni) and a number of loops of the acquisition windows (N); and
14) using the measured 90° hard pulse as excitation pulses of a pulse sequence, after t 1 /2, implementing the selective 180° pulse and the gradient (G 1 ), then implementing a PSYCHE element, and after another t 1 /2, implementing the gradient (G 1 ) and the gradient (G p ), then repeating an EPSI readout for N times and acquiring NMR signals, wherein:
after disentangling different signals, relevant J coupling constants are measured.

6. An NMR multi-dimensional method for measuring coupling constants within several coupling networks according to claim 5 , wherein:
the PSYCHE element includes two chirp pulses with small flip angle and opposite sweeping directions, and a simultaneously applied instance of the gradient (G 2 ),
the PSYCHE element is flanked by two of the spoiling gradients (G s ), and
the EPSI readout includes the gradient G a and gradient (G a ), and simultaneous samplings, of which a duration under one gradient is the duration (T a ).

7. An NMR multi-dimensional method for measuring coupling constants within several coupling networks according to claim 5 , wherein:
the number of the increments (ni) is set according to a required digital resolution in the indirect dimension: ni=SW 1 /v 1 , where v 1 is the required digital resolution in the indirect dimension and SW 1 is the spectral width in the indirect dimension.

8. An NMR multi-dimensional method for measuring coupling constants within several coupling networks according to claim 5 , wherein:
the number of loops of the acquisition windows (N) is set according to a required digital resolution in the direct dimension: N=SW 1D /v 2 , where v 2 is the required digital resolution in the direct dimension.

9. An NMR multi-dimensional method for measuring coupling constants within several coupling networks according to claim 5 , wherein:
the flip angle of the chirp pulses (β) is chosen for a balance between signal-to-noise ratio (SNR) and spectral purity, wherein a larger flip angle (β) results in higher SNR, but leads to stronger artifacts, and the flip angle (β) is set to 15°˜20°.

10. An NMR multi-dimensional method for measuring coupling constants within several coupling networks according to claim 5 , wherein:
the disentangling comprises:
for each t 1 , extracting data recorded under positive gradients and arranging the extracted data in a two-dimensionale (2D) matrix, thus obtaining a three-dimensional (3D), dataset;
processing the 3D dataset by a 3D Fourier transformation;
after extracting 2D spectra of relevant slices, obtaining 2D spectra respectivel

Assignees

• Univ Xiamen

Inventors

• Lin Yanqin
• Zeng Qing
• Chen Jinyong
• Chen Zhong

Classifications

• G01R33/4633 Primary

  Sequences for multi-dimensional NMR · CPC title

• G01N24/08 Primary

  by using nuclear magnetic resonance (G01N24/12 takes precedence) · CPC title

• G01R33/4625

  Processing of acquired signals, e.g. elimination of phase errors, baseline fitting, chemometric analysis · CPC title

• G01R33/4616

  using specific RF pulses or specific modulation schemes, e.g. stochastic excitation, adiabatic RF pulses, composite pulses, binomial pulses, Shinnar-le-Roux pulses, spectrally selective pulses not being used for spatial selection · CPC title

• G01R33/4608

  RF excitation sequences for enhanced detection, e.g. NOE, polarisation transfer, selection of a coherence transfer pathway · CPC title