Assay plates, reader systems and methods for luminescence test measurements

Patent metadata

Field	Value
Publication number	US-10782227-B2
Application number	US-202016845560-A
Country	US
Kind code	B2
Filing date	Apr 10, 2020
Priority date	Jun 29, 2001
Publication date	Sep 22, 2020
Grant date	Sep 22, 2020

Abstract

Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.

First claim

The invention claimed is:

1. An apparatus for measuring luminescence from a multi-well assay plate having a plurality of wells, said apparatus comprising:
(a) an imaging system comprising a detector, the imaging system configured to image one or more sectors of said plurality of wells in said multi-well assay plate and measure luminescence emitted therefrom, wherein the detector is selected from the group consisting of a camera, a photodetector, a photodiode, an avalanche photodiode, a charged-coupled device (CCD) chip, a CCD camera, a photomultiplier tube, a complementary metal-oxide-semiconductor (CMOS) detector, a film, a phosphorescent material, and an intensifier; and
(b) a plurality of electrical connectors adapted to connect a source of electrical energy to said one or more sectors of said plurality of wells, wherein said electrical connectors are positioned on an electrical connector surface and configured to substantially vertically align said plurality of electrical connectors with a bottom surface of said multi-well assay plate and contact said bottom surface of said multi-well plate.

2. The apparatus of claim 1 , wherein said electrical connector surface is configured to substantially vertically align said plurality of electrical connectors, said one or more sectors of said plurality of wells and said imaging system.

3. The apparatus of claim 1 , comprising two or more electrical connectors.

4. The apparatus of claim 1 , further comprising a multi-well plate holder and said electrical connectors are positioned on a top surface of said plate holder.

5. The apparatus of claim 1 , wherein said plurality of electrical connectors comprise one or more working electrical connectors and two or more counter electrical connectors.

6. The apparatus of claim 1 , wherein bottom surface comprises one or more contact surfaces and said plurality of electrical connectors are configured to contact said plate bottom at said one or more contact surfaces.

7. The apparatus of claim 1 , wherein said imaging system further comprises a mounting system for positioning the detector relative to said one or more sectors of said plate.

8. The apparatus of claim 1 , further comprising one or more additional subsystems including: (i) a plate translation subsystem; (ii) a plate shaking subsystem; (iii) a plate storage subsystem; (iv) a reagent handling subsystem; (v) a reagent delivery subsystem; and (vi) combinations thereof.

9. The apparatus of claim 1 , wherein said detector is a CCD camera or chip.

10. The apparatus of claim 1 , wherein said detector is a photodiode or avalanche photodiode.

11. The apparatus of claim 1 , wherein said detector comprises an array of photodiodes.

12. A multi-well assay plate having a plurality of wells, said plate comprising:
a plurality of electrical contacts supported on a bottom surface of said multiwell plate, each electrical contact is adapted to connect a source of electrical energy to, and substantially vertically aligned with, one or more sectors of said plurality of wells.

13. The plate of claim 12 , wherein the plate is configured for imaging by an imaging system comprising a detector, the imaging system configured to image said one or more sectors of said plurality of wells in said multi-well assay plate and measure luminescence emitted therefrom, wherein the detector is selected from the group consisting of a camera, a photodiode, an avalanche photodiode, a charged-coupled device (CCD) chip, a CCD camera, a photomultiplier tube, a complementary metal-oxide-semiconductor (CMOS) detector, a film, a phosphorescent material, and an intensifier.

14. The plate of claim 13 , wherein said bottom surface of said multiwell plate is configured to substantially vertically align said plurality of electrical contacts, said one or more sectors of said plurality of wells and said imaging system.

15. The plate of claim 12 , comprising two or more electrical contacts.

16. The plate of claim 12 , further comprising a multi-well plate holder and said electrical contacts are positioned on a top surface of said plate holder.

17. The plate of claim 12 , wherein said plurality of electrical contacts comprise one or more working electrical contacts and two or more counter electrical contacts.

18. The plate of claim 12 , comprising seven electrical contacts.

19. The plate of claim 12 , wherein said bottom surface comprises one or more contact surfaces and said plurality of electrical contacts are configured to contact said plate bottom at said one or more contact surfaces.

20. The plate of claim 13 , wherein said imaging system further comprises a mounting system for positioning the detector relative to said one or more sectors of said plate.

21. The plate of claim 13 , further comprising one or more additional subsystems including: (i) a plate translation subsystem; (ii) a plate shaking subsystem; (iii) a plate storage subsystem; (iv) a reagent handling subsystem, (v) a reagent delivery subsystem; and (vi) combinations thereof.

22. The plate of claim 13 , wherein said detector is a CCD camera or chip.

23. The plate of claim 13 , wherein said detector is a photodiode or avalanche photodiode.

24. The plate of claim 13 , wherein said detector comprises an array of photodiodes.

25. The plate of claim 12 , wherein said electrical contacts are spring-loaded.

26. The plate of claim 12 , wherein each of said plurality of wells comprises an inner bottom surface.

27. The plate of claim 26 , further comprising a plurality of electrodes, wherein each inner bottom surface of each of said plurality of wells comprises an electrode of the plurality of electrodes.

28. The plate of claim 27 , wherein each of said plurality of electrodes are connected to a portion of the bottom surface of said multiwell plate.

29. The plate of claim 12 , wherein the plurality of electrical contacts are configured to receive electrical energy from the electrical connectors of claim 1 .

30. The apparatus of claim 1 , wherein said electrical connectors are positioned on, and extend a distance from, the electrical connector surface.

Assignees

• Meso Scale Technologies Llc

Inventors

• Wohlstadter Jacob N
• Glezer Eli
• Wilbur James
• Sigal George
• Johnson Kent
• Clinton Charles
• Kishbaugh Alan
• Jeffrey-Coker Bandele
• Debad Jeff D
• Fischer Alan B

Classifications

• G01N21/69

  specially adapted for fluids {, e.g. molten metal} · CPC title

• Y10T29/49155

  Manufacturing circuit on or in base · CPC title

• Y10T83/04

  Processes · CPC title

• G01N2035/0494

  Detecting or compensating piositioning errors · CPC title

• G01N2035/0425

  Stacks, magazines or elevators for plates · CPC title