Assay plates, reader systems and methods for luminescence test measurements

Patent metadata

Field	Value
Publication number	US-10539501-B2
Application number	US-201715587968-A
Country	US
Kind code	B2
Filing date	May 5, 2017
Priority date	Jun 29, 2001
Publication date	Jan 21, 2020
Grant date	Jan 21, 2020

Abstract

Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.

First claim

The invention claimed is:

1. A multi-well plate comprising:
(a) a first electrically conductive layer partitioned into two or more electrically isolated sectors;
(b) an insulating layer having a plurality of insulating layer openings;
(c) a second electrically conductive layer on said insulating layer having a plurality of second electrically conductive layer openings; and
(d) a plate top having a plurality of plate top openings;

wherein said insulating layer is between said first electrically conductive layer and said plate top and wherein said insulating layer openings, said second electrically conductive layer openings and said plate top openings are aligned forming wells and wherein said two or more electrically isolated sectors are aligned with said plate top openings thereby forming independently addressable sectors of jointly addressable wells.

2. A multi-well plate for conducting electrode induced luminescence assays comprising: (a) a first electrically conductive layer; (b) an insulating layer having a plurality of insulating layer openings; (c) a second electrically conductive layer on said insulating layer having a plurality of second electrically conductive layer openings, said second electrically conductive layer being partitioned into two or more electrically isolated sectors; and (d) a plate top having a plurality of plate top openings; wherein said insulating layer is between said first electrically conductive layer and plate top and wherein said insulating layer openings, said second electrically conductive layer openings and said plate top openings are aligned forming wells for conducting said electrode induced luminescence assays.

3. A multi-well plate for conducting electrode induced luminescence assays comprising: (a) a first electrically conductive layer partitioned into two or more electrically isolated sectors; (b) an insulating layer having a plurality of insulating layer openings; (c) a second electrically conductive layer on said insulating layer having a plurality of second electrically conductive layer openings, said second electrically conductive layer being partitioned into two or more electrically isolated sectors; and (d) a plate top having a plurality of plate top openings; wherein said insulating layer is between said first electrically conductive layer and plate top and wherein said insulating layer openings, said second electrically conductive layer openings and said plate top openings are aligned forming wells for conducting said electrode induced luminescence assays.

4. A multi-well plate bottom comprising:
(a) a substrate having a top surface and a bottom surface;
(b) a plurality of patterned working electrodes on said top surface;
(c) a plurality of patterned counter electrodes on said top surface each of said patterned counter electrodes being associated with corresponding patterned working electrodes; and
(d) two or more independently addressable sectors, each sector having two or more independently addressable contacts on said bottom surface, each of said contacts corresponding to one or more electrodes within wells within one of said sectors.

5. A multi-well plate bottom comprising:
(a) a substrate having a top surface and a bottom surface;
(b) a plurality of patterned working electrodes on said top surface;
(c) a plurality of patterned counter electrodes on said top surface, each of said patterned counter electrodes being associated with corresponding patterned working electrodes; and
(d) two or more independently addressable contact sectors on said bottom surface, each of said contact sectors corresponding to an electrode sector comprising one or more of said plurality of patterned working electrodes and one or more of said plurality of patterned counter electrodes on said top surface and including a plurality of conductive contact surfaces;
wherein said plurality of conductive contact surfaces include:
(i) a first conductive contact surface located within a first contact region, said first conductive contact surface being electrically connected to said one or more corresponding patterned working electrodes on said top surface; and
(ii) a second conductive contact surface located within a second contact region, said second conductive contact surface being electronically connected to said one or more corresponding patterned counter electrodes on said top surface;
wherein said first conductive contact surface and said second conductive contact surface are electrically isolated from each other.

6. A multi-well plate comprising the plate bottom of claim 4 , and a plate top affixed on said top surface of said plate bottom forming said multi-well plate.

7. The multi-well plate of claim 1 , wherein said first electrically conductive layer comprises carbon.

8. The multi-well plate of claim 1 , wherein said first electrically conductive layer comprises a carbon layer on a silver layer.

9. The multi-well plate of claim 1 , wherein said first electrically conductive layer comprises carbon particles.

10. The multi-well plate of claim 1 , wherein said first electrically conductive layer comprises carbon fibrils or carbon black.

11. The multi-well plate of claim 1 , wherein said first electrically conductive layer comprises carbon fibrils.

12. The multi-well plate of claim 1 , wherein said first electrically conductive layer is located at the center of the well bottom.

13. The multi-well plate of claim 1 , wherein said first electrically conductive layer comprises one or more binding reagents immobilized thereon.

14. The multi-well plate of claim 1 , wherein said first electrically conductive layer comprises one or more assay domains.

15. The multi-well plate of claim 14 , wherein said assay domains comprise one or more immobilized reagents selected from: antibodies, fragments of antibodies, proteins, enzymes, enzyme substrates, inhibitors, cofactors, antigens, haptens, lipoproteins, liposaccharides, cells, sub-cellular components, cell receptors, viruses, nucleic acids, antigens, lipids, glycoproteins, carbohydrates, peptides, amino acids, hormones, protein-binding ligands, pharmacological agents, luminescent labels or combinations thereof.

16. The multi-well plate of claim 15 , wherein said immobilized reagents comprise proteins, nucleic acids, or combinations thereof.

17. The multi-well plate of claim 4 , wherein said substrate comprises a plastic.

18. The multi-well plate of claim 4 , wherein said substrate comprises metal particles incorporated into or on said plastic.

19. The multi-well plate of claim 4 , wherein said substrate comprises carbon particles incorporated into or on said plastic.

20. The multi-well plate of claim 4 , wherein said substrate comprises carbon fibrils incorporated into or on said plastic.

21. The multi-well plate of claim 4 , wherein said substrate is substantially free of silicon and silicone.

22. The multi-well plate of claim 4 , wherein said substrate comprises a porous material.

23. The multi-well plate of claim 1 , wherein said wells comprise an inner bottom surface and said inner surface is oxidized to expose a conductive surface.

24. The multi-well plate of claim 1 , wherein said wells comprise an inner bottom surface and said inner bottom surface is oxidized to produce functionality for coupling with an assay component.

25. The multi-well plate of claim 1 , wherein said wells are treated with a bifunctional reagent for coupling.

Assignees

• Meso Scale Technologies Llc

Inventors

• Wohlstadter Jacob N
• Glezer Eli
• Wilbur James
• Sigal George
• Johnson Kent
• Clinton Charles
• Kishbaugh Alan
• Jeffrey-Coker Bandele
• Debad Jeff D
• Fischer Alan B

Classifications

• B01L9/56

  Means for indicating position of a recipient or sample in an array · CPC title

• B01L2300/0851

  Bottom walls · CPC title

• G01N2035/0494

  Detecting or compensating piositioning errors · CPC title

• G01N21/253 Primary

  for batch operation, i.e. multisample apparatus (analytical automats G01N35/00) · CPC title

• Y10T83/04

  Processes · CPC title