Inlet instrumentation for ion analyser coupled to rapid evaporative ionisation mass spectrometry (“REIMS”) device

Patent metadata

Field	Value
Publication number	US-10777397-B2
Application number	US-201615555720-A
Country	US
Kind code	B2
Filing date	Mar 7, 2016
Priority date	Mar 6, 2015
Publication date	Sep 15, 2020
Grant date	Sep 15, 2020

Abstract

An apparatus is disclosed comprising a first device for generating aerosol, smoke or vapour from one or more regions of a target, an inlet conduit to an ion analyser or mass spectrometer, the inlet conduit having an inlet through which the aerosol, smoke or vapour passes, and a Venturi pump arrangement arranged and adapted to direct the aerosol, smoke or vapour towards the inlet.

First claim

The invention claimed is:

1. An apparatus comprising:
a first device for generating aerosol, smoke or vapour from one or more regions of a target;
an inlet conduit to an ion analyser or mass spectrometer, said inlet conduit having an inlet through which said aerosol, smoke or vapour passes;
a Venturi pump arrangement arranged and adapted to direct said aerosol, smoke or vapour towards said inlet, wherein said Venturi pump arrangement is arranged and adapted to direct said aerosol, smoke or vapour onto a deflection device or surface prior to said aerosol, smoke or vapour passing through said inlet, wherein said deflection device comprises a hollow member having a first side and a second side, wherein the first side is solid and the second side comprises one or more apertures arranged and adapted to allow said aerosol, smoke or vapour to pass therethrough and wherein said Venturi pump arrangement is arranged and adapted to direct said aerosol, smoke or vapour onto the first surface of said deflection device;
a matrix conduit for introducing and mixing a matrix with said aerosol, smoke or vapour prior to said aerosol, smoke or vapour passing through the inlet; and
a collision surface located within a vacuum chamber and arranged and adapted such that said aerosol, smoke or vapour is caused to impact upon said collision surface so as to generate a plurality of analyte ions.

2. The apparatus as claimed in claim 1 , wherein said one or more apertures are in fluid communication with a cavity or passage within said hollow member, and said inlet is in fluid communication with said cavity or passage.

3. The apparatus as claimed in claim 1 , wherein said matrix conduit is in fluid communication with said cavity or passage.

4. The apparatus as claimed in claim 1 , wherein said matrix conduit and/or said inlet conduit and/or said cavity or passage are aligned substantially co-axially with one another.

5. The apparatus as claimed in claim 1 , wherein said Venturi pump arrangement comprises an elongated portion having an outlet through which said aerosol, smoke or vapour passes, and said elongated portion has a longitudinal axis that is perpendicular, or substantially perpendicular to a longitudinal axis of said cavity or passage and/or said inlet conduit and/or said matrix conduit.

6. The apparatus as claimed in claim 1 , wherein said first device comprises an ambient ion source.

7. The apparatus as claimed in claim 1 , wherein said first device comprises an ion source selected from the group consisting of: (i) a rapid evaporative ionisation mass spectrometry (“REIMS”) ion source; (ii) a desorption electrospray ionisation (“DESI”) ion source; (iii) a laser desorption ionisation (“LDI”) ion source; (iv) a thermal desorption ion source; (v) a laser diode thermal desorption (“LDTD”) ion source; (vi) a desorption electro-flow focusing (“DEFFI”) ion source; (vii) a dielectric barrier discharge (“DBD”) plasma ion source; (viii) an Atmospheric Solids Analysis Probe (“ASAP”) ion source; (ix) an ultrasonic assisted spray ionisation ion source; (x) an easy ambient sonic-spray ionisation (“EASI”) ion source; (xi) a desorption atmospheric pressure photoionisation (“DAPPI”) ion source; (xii) a paperspray (“PS”) ion source; (xiii) a jet desorption ionisation (“JeDI”) ion source; (xiv) a touch spray (“TS”) ion source; (xv) a nano-DESI ion source; (xvi) a laser ablation electrospray (“LAESI”) ion source; (xvii) a direct analysis in real time (“DART”) ion source; (xviii) probe electrospray ionisation (“PESI”) ion source; (xix) a solid-probe assisted electrospray ionisation (“SPA-ESI”) ion source; (xx) a cavitron ultrasonic surgical aspirator (“CUSA”) ion source; (xxi) a focussed or unfocussed ultrasonic ablation ion source; (xxii) a microwave resonance ion source; and (xxiii) a pulsed plasma RF dissection device.

8. The apparatus as claimed in claim 1 , wherein said first device comprises a laser source for irradiating said target with laser light to generate said aerosol, smoke or vapour.

9. The apparatus as claimed in claim 1 , wherein said matrix comprises polar molecules, water, one or more alcohols, methanol, ethanol, isopropanol, acetone or acetonitrile.

10. The apparatus as claimed in claim 1 , further comprising a mass analyser and/or ion mobility analyser arranged and adapted to mass analyse and/or ion mobility analyse said analyte ions in order to obtain mass spectrometric and/or ion mobility data.

11. A method comprising:
generating aerosol, smoke or vapour from one or more regions of a target;
providing an inlet conduit to an ion analyser or mass spectrometer, said inlet conduit having an inlet through which said aerosol, smoke or vapour passes;
using a Venturi pump arrangement to direct said aerosol, smoke or vapour towards said inlet, wherein said Venturi pump arrangement directs said aerosol, smoke or vapour onto a deflection device or surface prior to said aerosol, smoke or vapour passing through said inlet, wherein said deflection device comprises a hollow member having a first side and a second side, wherein the first side is solid and the second side comprises one or more apertures arranged and adapted to allow said aerosol, smoke or vapour to pass therethrough and wherein said Venturi pump arrangement is arranged and adapted to direct said aerosol, smoke or vapour onto the first surface of said deflection device;
introducing and mixing a matrix with said aerosol, smoke or vapour prior to said aerosol, smoke or vapour passing through the inlet; and
providing a collision surface located within a vacuum chamber such that said aerosol, smoke or vapour impacts upon said collision surface so as to generate a plurality of analyte ions.

12. The method as claimed in claim 11 , wherein said first device comprises an ambient ion source.

13. The method as claimed in claim 11 , wherein said first device comprises an ion source selected from the group consisting of: (i) a rapid evaporative ionisation mass spectrometry (“REIMS”) ion source; (ii) a desorption electrospray ionisation (“DESI”) ion source; (iii) a laser desorption ionisation (“LDI”) ion source; (iv) a thermal desorption ion source; (v) a laser diode thermal desorption (“LDTD”) ion source; (vi) a desorption electro-flow focusing (“DEFFI”) ion source; (vii) a dielectric barrier discharge (“DBD”) plasma ion source; (viii) an Atmospheric Solids Analysis Probe (“ASAP”) ion source; (ix) an ultrasonic assisted spray ionisation ion source; (x) an easy ambient sonic-spray ionisation (“EASI”) ion source; (xi) a desorption atmospheric pressure photoionisation (“DAPPI”) ion source; (xii) a paperspray (“PS”) ion source; (xiii) a jet desorption ionisation (“JeDI”) ion source; (xiv) a touch spray (“TS”) ion source; (xv) a nano-DESI ion source; (xvi) a laser ablation electrospray (“LAESI”) ion source; (xvii) a direct analysis in real time (“DART”) ion source; (xviii) probe electrospray ionisation (“PESI”) ion source; (xix) a solid-probe assisted electrospray ionisation (“SPA-ESI”) ion source; (xx) a cavitron ultrasonic surgical aspirator (“CUSA”) ion source; (xxi) a focussed or unfocussed ultrasonic ablation ion source; (xxii) a microwave resonance ion source; and (xxiii) a pulsed plasma RF dissection device.

14. The method as claimed in claim 11 , further comprising irradiating said target with laser light to generate said aerosol, smoke or vapour.

15. The method as claimed in claim 11 , wherein said matrix comprises polar molecules, water, one or more alcohols, methanol, ethanol, isopropanol, acetone or acetonitrile.

16. The method as claimed in claim 11 , further comprising mass an

Assignees

• Micromass Ltd

Inventors

• Takáts Zoltán
• Balog Júlia
• Pringle Steven Derek
• Karancsi Tamás
• Morris Michael Raymond
• Gödörhazy Lajos
• Szalay Dániel
• Simon Dániel

Classifications

• G01N33/92

  involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)} · CPC title

• A61B2017/320074

  blade · CPC title

• A61B10/0283

  with vacuum aspiration, e.g. caused by retractable plunger or by connected syringe (A61B10/025, A61B10/0266 take precedence) · CPC title

• A61B10/0233

  Pointed or sharp biopsy instruments · CPC title

• H01J49/068

  Mounting, supporting, spacing, or insulating electrodes · CPC title