System and method for rapid evaporative ionization of liquid phase samples

Patent metadata

Field	Value
Publication number	US-9281174-B2
Application number	US-201214368796-A
Country	US
Kind code	B2
Filing date	Dec 28, 2012
Priority date	Dec 28, 2011
Publication date	Mar 8, 2016
Grant date	Mar 8, 2016

Abstract

According to some embodiments, systems and methods for rapid evaporation of liquid phase samples are provided. The method includes directing liquid samples to a thermal evaporation ionizing device, thermally evaporating the liquid samples to create gaseous molecular ions, and directing the gaseous molecular ions to an ion analyzer to analyze and provide information regarding the chemical composition of the liquid samples.

First claim

What is claimed is:

1. A method for analyzing liquid phase samples, comprising
guiding a liquid sample to an ionizing device; and
thermally evaporating the liquid sample with the ionizing device at a rate sufficient to convert one or more molecular components of the liquid sample to one or more gaseous ions and neutral particles.

2. The method of claim 1 , further comprising exposing the neutral particles to a secondary ion source such that the neutral particles interact with one or more charged particles produced by the secondary ion source to convert at least one of the neutral particles to a gaseous ion, said secondary ion source chosen from the group consisting of an electrospray ion source, a corona discharge ionization source, a glow discharge ionization source, an atmospheric pressure chemical ionization source, a dielectric barrier discharge, and an electromagnetic ionization source.

3. The method of claim 1 , further comprising analyzing said gaseous ions with an ion analyzer device to provide information on the chemical composition of the liquid sample.

4. The method of claim 3 , further comprising applying an electrostatic potential between the ionizing device and the ion analyzer device.

5. The method of claim 3 , wherein the ion analyzer device is a mass spectrometer or ion mobility spectrometer.

6. The method of claim 3 , wherein the liquid sample is a biological fluid sample and said information is used to establish a medical diagnosis.

7. The method of claim 1 , wherein guiding comprises flowing the liquid sample through a conduit upstream of the ionizing device.

8. The method of claim 7 , wherein flowing the liquid sample comprises continuously flowing the liquid sample to the ionizing device.

9. The method of claim 1 , wherein thermally evaporating the liquid sample comprises applying an electric current to the liquid sample as the liquid sample passes through a gap between a pair of electrodes.

10. The method of claim 9 , wherein applying an electric current comprises applying an alternating current.

11. The method of claim 9 , wherein applying an electric current comprises applying a direct current.

12. The method of claim 9 , wherein applying the electric current to the liquid sample comprises applying electric power of between about 1 W and about 100 W to the liquid sample.

13. The method of claim 9 , further comprising directing at least the one or more neutral particles through a post-ionization device such that the one or more neutral particles interact with charged particles produced by the post-ionization device to convert at least one of the neutral particles to gaseous ions.

14. The method of claim 1 , wherein thermally evaporating the liquid sample comprises heating the liquid sample when it contacts a heated inner surface of a cylinder of the ionizing device into which the liquid sample flows, said surface heated to temperature above the boiling point of the liquid sample and below the Leidenfrost temperature of the liquid sample.

15. The method of claim 14 , further comprising exposing the one or more neutral particles to charged particles produced by an electrospray secondary ionization device to convert at least one of the neutral particles to a gaseous ion.

16. The method of claim 1 , wherein thermally evaporating the liquid sample comprises heating the liquid sample via contact with a heated surface of the ionizing device onto which the liquid sample is dripped, the heated surface heated to a temperature above the boiling point of the liquid sample and below the Leidenfrost temperature of the liquid sample.

17. The method of claim 16 , further comprising exposing the one or more neutral particles to charged particles produced by an electrospray secondary ionization device to convert at least one of the neutral particles to gaseous ions.

18. The method of claim 1 , wherein thermally evaporating the liquid sample comprises heating the liquid sample via electromagnetic radiation from one or more lasers as said liquid sample passes through a focal point of the one or more lasers.

19. The method of claim 1 , wherein thermally evaporating the liquid sample comprises heating the liquid sample via an electric current between a pair of electrodes inserted into a microwell of a microtiter plate into which the liquid sample is delivered, the electrode being at least partially submerged in the liquid sample.

20. A system for analyzing liquid phase samples, comprising
a conduit configured to guide a liquid sample therethrough;
a thermal evaporation ionizing device configured to receive the liquid sample from the conduit, the ionizing device configured to thermally evaporate the liquid sample at a rate sufficient to convert one or more molecular components of the liquid sample into one or more gaseous ions and neutral particles; and
a transport device configured to receive the one or more gaseous ions from the ionizing device.

21. The system of claim 20 , wherein the liquid sample is a biological fluid sample.

22. The system of claim 20 , further comprising an ion analyzer device configured receive the one or more gaseous ions from the transport device and to analyze said gaseous ions to provide information on the chemical composition of the liquid sample.

23. The system of claim 22 , wherein the ion analyzer device is a mass spectrometer or ion mobility spectrometer.

24. The system of claim 20 , further comprising a secondary ion source configured to produce one or more charged particles configured to interact with the neutral particles so as to convert at least one of the neutral particles to a gaseous ion, said secondary ion source chosen from the group consisting of an electrospray ion source, a corona discharge ionization source, a glow discharge ionization source, an atmospheric pressure chemical ionization source, a dielectric barrier discharge, and an electromagnetic ionization source.

25. The system of claim 20 , wherein the ionizing device comprises a pair of electrodes defining a gap through which the liquid sample passes, the electrodes configured to apply an electric current to the liquid sample as it passes through the gap.

26. The system of claim 25 , wherein the liquid sample passes through the gap in the electrodes in a continuous flow.

27. The system of claim 25 , wherein the electrodes are configured to apply electric power of between about 1 W and about 100 W to the liquid sample as it passes through the gap between the electrodes.

28. The system of claim 20 , wherein the ionizing device comprises a cylinder comprising a heater and an opening extending through the cylinder, the cylinder having an inner cylindrical surface that receives the liquid sample thereon to thermally evaporate the liquid sample via contact heating, wherein the heater heats the inner cylindrical surface to a temperature above the boiling point of the liquid sample and below the Leidenfrost temperature of the liquid sample.

29. The system of claim 28 , further comprising an electrospray ionization device configured to direct an electrospray into the opening of the cylinder such that charged particles produced by the electrospray ionization device interact with neutral particles generated via said contact heating of the liquid sample to convert at least one of the neutral particles to a gaseous ion.

30. The system o

Assignees

• Micromass Ltd

Inventors

• Takats Zoltan

Classifications

• G01N27/626

  using heat to ionise a gas · CPC title

• G01N33/493

  urine · CPC title

• A61B5/4845

  Toxicology, e.g. by detection of alcohol, drug or toxic products (A61B5/4848 takes precedence) · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• H01J49/0431

  for liquid samples (interfaces to liquid chromatographs G01N30/7233) · CPC title