Method of producing D-xylonate and coryneform bacterium

Patent metadata

Field	Value
Publication number	US-10760104-B2
Application number	US-201716304144-A
Country	US
Kind code	B2
Filing date	May 19, 2017
Priority date	Jun 25, 2016
Publication date	Sep 1, 2020
Grant date	Sep 1, 2020

Abstract

The invention relates to a method of producing D-xylonate from D-xylose, which includes converting D-xylose to D-xylonate from a coryneform bacterium in which the activity of the iolR gene is reduced or completely switched off compared with the wild type or a mutation of the wild type, or the iolR gene is completed or partially deleted, as well as to a bacterium for carrying out the method.

First claim

The invention claimed is:

1. A method for microbial production of D-xylonate from D-xylose,
comprising
converting D-xylose to D-xylonate in a coryneform bacterium in which the activity of the iolR gene is reduced or completely switched off compared with the wild type or a mutant of the wild type, or the iolR gene is completely or partially deleted, wherein the wild type of the coryneform bacterium is Corynebacterium or Brevibacterium.

2. The method of claim 1 ,
wherein
the coryneform bacterium is not recombinantly modified.

3. The method of claim 1 ,
wherein
the coryneform bacterium is selected from the group consisting of Corynebacterium and Brevibacterium.

4. The method of claim 3 ,
wherein
the coryneform bacterium is selected from the group consisting of
Corynebacterium glutamicum ATCC13032
Corynebacterium acetoglutamicum ATCC15806
Corynebacterium acetoacidophilum ATCC13870
Corynebacterium thermoaminogenes FERM BP-1539
Brevibacterium flavum ATCC14067
Brevibacterium lactofermentum ATCC13869 and
Brevibacterium divaricatum ATCC14020.

5. The method of claim 1 ,
wherein
the iolR gene is partially or completely deactivated by using myo-inositol as a substrate/inductor of iolR, by inserting a vector or another gene sequence into the gene segment of the iolR gene in which the modification affects the activity of the iolR gene, or by modifying signal structures of gene expression.

6. The method of claim 1 ,
wherein
D-xylonate is produced on a defined, D-xylose-containing medium.

7. A modified coryneform bacterium,
wherein
the activity of the iolR gene is reduced or completely switched off compared with the wild type or a mutant of the wild type or the iolR gene is completely or partially deleted, wherein the wild type of the coryneform bacterium is Corynebacterium or Brevibacterium.

8. The modified coryneform bacterium of claim 7 , wherein the modified coryneform bacterium is not recombinantly modified.

9. The modified coryneform bacterium of claim 7 ,
wherein
the modified coryneform bacterium is selected from the group consisting of Corynebacterium and Brevibacterium.

10. The modified coryneform bacterium of claim 7 ,
wherein
the modified coryneform bacterium is selected from the group consisting of
Corynebacterium glutamicum ATCC13032
Corynebacterium acetoglutamicum ATCC15806
Corynebacterium acetoacidophilum ATCC13870
Corynebacterium thermoaminogenes FERM BP-1539
Brevibacterium flavum ATCC14067
Brevibacterium lactofermentum ATCC13869 and
Brevibacterium divaricatum ATCC14020.

11. The modified coryneform bacterium of claim 7 ,
wherein
the iolR gene is partially or completely deactivated by using myo-inositol as a substrate/inductor of iolR, by inserting a vector or another sequence into the gene segment of the iolR gene, or by modifying the signal structures of gene expression.

12. The method of claim 3 ,
wherein the coryneform bacterium is selected from the group consisting of Corynebacterium glutamicum, Corynebacterium acetoglutamicum, Corynebacterium thermoaminogenes, Brevibacterium flavum, Brevibacterium lactofermentum and Brevibacterium divaricatum.

13. The modified coryneform bacterium of claim 9 ,
wherein the modified coryneform bacterium is selected from the group consisting of Corynebacterium glutamicum, Corynebacterium acetoglutamicum, Corynebacterium thermoaminogenes, Brevibacterium flavum, Brevibacterium lactofermentum and Brevibacterium divaricatum.

14. The method of claim 5 , wherein the signal structures of gene expression include repressor genes, activator genes, operators, promoters, attenuators, ribosome bonding points, start codons, terminators, mutations in the form of transitions, transversions, insertions or deletions.

15. The method of claim 1 , wherein the activity of the iolR gene is reduced or completely switched off compared with the wild type bacterium, wherein the wild type of the coryneform bacterium is Corynebacterium or Brevibacterium.

16. The modified coryneform bacterium of claim 7 , wherein the activity of the iolR gene is reduced or completely switched off compared with the wild type bacterium, wherein the wild type of the coryneform bacterium is Corynebacterium or Brevibacterium.

17. The method of claim 1 , wherein the activity of the iolR gene is reduced or completely switched off compared with a mutant of the wild type bacterium, wherein the wild type of the coryneform bacterium is Corynebacterium or Brevibacterium.

18. The modified coryneform bacterium of claim 7 , wherein the activity of the iolR gene is reduced or completely switched off compared with a mutant of the wild type bacterium, wherein the wild type of the coryneform bacterium is Corynebacterium or Brevibacterium.

19. The method of claim 1 , wherein the iolR gene is completely or partially deleted.

20. The modified coryneform bacterium of claim 7 , wherein the iolR gene is completely or partially deleted.

Assignees

• Forschungszentrum Juelich Gmbh

Inventors

• Noack Stephan
• Radek Andreas
• Marienhagen Jan
• Krumbach Karin

Classifications

• C07K14/34

  from Corynebacterium (G) · CPC title

• C12N1/36

  Adaptation or attenuation of cells · CPC title

• C12P7/58 Primary

  Aldonic, ketoaldonic or saccharic acids (uronic acids C12P19/00) · CPC title