Automated bioreactor system, system for automatically implementing protocol for decellularizing organ, and waste decontamination system

Patent metadata

Field	Value
Publication number	US-10757932-B2
Application number	US-201515508005-A
Country	US
Kind code	B2
Filing date	Sep 1, 2015
Priority date	Sep 2, 2014
Publication date	Sep 1, 2020
Grant date	Sep 1, 2020

Abstract

An automated bioreactor system for decellularizing an organ includes a main chamber for containing the organ. The system further includes a reagent chamber containing a liquid phase reagent. A reagent conduit delivers the liquid phase reagent to the main chamber, and a perfusion conduit delivers the reagent from the reagent outlet in the main chamber into the organ. A perfusion pump drives the flow of the reagent. A perfusion pressure sensor detects a pressure of the flowing reagent. A control system controls the perfusion pump to drive the flow of the reagent based on a received input representative of a desired pressure and a received input of the detected pressure. The control system may automatically perform all of the steps of a decellularization protocol based on sensor input. An automated waste decontamination system may also be provided.

First claim

What is claimed is:

1. An automated bioreactor system for decellularizing an organ, comprising:
a main chamber configured to contain the organ, the main chamber having at least one reagent inlet, at least one reagent outlet, and at least one perfusion inlet;
at least one reagent chamber containing a liquid phase reagent;
at least one reagent conduit configured to deliver the liquid phase reagent from the at least one reagent chamber to the main chamber through the at least one reagent inlet;
at least one reagent valve configured to control a flow of the liquid phase reagent through the at least one reagent conduit;
at least one perfusion conduit configured to deliver the liquid phase reagent from the at least one reagent outlet into the organ contained in the main chamber through the at least one perfusion inlet;
at least one perfusion pump configured to drive the flow of the liquid phase reagent through the at least one perfusion conduit;
at least one perfusion pressure sensor configured to detect a pressure of the liquid phase reagent flowing through the at least one perfusion conduit;
a weight sensor configured to detect a weight contained in the main chamber; and
a control system configured to:
initiate a chamber fill process comprising outputting a signal to open a valve associated with the reagent conduit to fill the main chamber with a predetermined amount of liquid phase reagent;

receive an input of the weight detected by the weight sensor;
output a signal to control the at least one reagent valve based on the detected weight;
receive an input representative of a desired pressure of the liquid phase reagent flowing through the at least one perfusion conduit during a perfusion process;
after the chamber fill process is complete, initiate the perfusion process comprising:
receiving an input of the pressure detected by the at least one perfusion pressure sensor; and
outputting a signal to control the at least one perfusion pump to drive the flow of the liquid phase reagent based on the received input representative of the desired pressure and the received input of the detected pressure.

2. The automated bioreactor system of claim 1 , further comprising:
at least one reagent pump configured to drive the flow of the liquid phase reagent through the at least one reagent conduit.

3. The automated bioreactor system of claim 2 , wherein the control system calculates a volume of the liquid phase reagent contained in the main chamber based on the detected weight and a density of the liquid phase reagent.

4. The automated bioreactor system of claim 1 , wherein the input representative of the desired pressure is at least one of a pressure and/or a flow rate.

5. The automated bioreactor system of claim 1 , wherein the at least one perfusion pressure sensor is configured to detect a pressure at the at least one perfusion inlet.

6. The automated bioreactor system of claim 1 , further comprising:
at least one reagent valve configured to control a flow of the liquid phase reagent through the at least one reagent conduit,
wherein the reagent conduit is flexible and the at least one reagent valve is a pinch valve that applies a force to the flexible reagent conduit to impede flow of the liquid phase reagent.

7. The automated bioreactor system of claim 1 , further comprising:
at least one temperature sensor configured to detect a temperature within the main chamber; and
at least one temperature control system configured to adjust the temperature within the main chamber,
wherein the control system is further configured to:
receive an input of the temperature detected by the at least one temperature sensor; and
output a signal to control the temperature control system to adjust the temperature in the main chamber.

8. An automated bioreactor system for decellularizing an organ, comprising:
a main chamber configured to contain the organ, the main chamber having at least one reagent inlet, at least one reagent outlet, and at least one perfusion inlet;
at least one reagent chamber containing a liquid phase reagent;
at least one reagent conduit configured to deliver the liquid phase reagent from the at least one reagent chamber to the main chamber through the at least one reagent inlet;
at least one temperature sensor configured to detect a temperature within the main chamber;
at least one temperature control system configured to adjust the temperature within the main chamber;
at least one perfusion conduit configured to deliver the liquid phase reagent from the at least one reagent outlet into the organ contained in the main chamber through the at least one perfusion inlet;
at least one perfusion pump configured to drive the flow of the liquid phase reagent through the at least one perfusion conduit;
at least one perfusion pressure sensor configured to detect a pressure of the liquid phase reagent flowing through the at least one perfusion conduit; and
a control system configured to:
initiate a chamber fill process comprising outputting a signal to open a valve associated with the reagent conduit to fill the main chamber with a predetermined amount of liquid phase reagent;
receive an input representative of a desired pressure of the liquid phase reagent flowing through the at least one perfusion conduit during a perfusion process; and
after the chamber fill process is complete, initiate the perfusion process comprising:
receiving an input of the pressure detected by the at least one perfusion pressure sensor;
outputting a signal to control the at least one perfusion pump to drive the flow of the liquid phase reagent based on the received input representative of the desired pressure and the received input of the detected pressure;
receiving an input of the temperature detected by the at least one temperature sensor; and
outputting a signal to control the temperature control system to adjust the temperature in the main chamber.

Assignees

• United Therapeutics Corp

Inventors

• Bonvillain Ryan
• Cheadle John
• Peterson Thomas

Classifications

• A61L2/022

  Filtration · CPC title

• A01N1/143 Primary

  for organ perfusion · CPC title

• G01K13/02

  for measuring temperature of moving fluids or granular materials capable of flow · CPC title

• G01L7/00

  Measuring the steady or quasi-steady pressure of a fluid or a fluent solid material by mechanical or fluid pressure-sensitive elements ({G01L11/004 takes precedence;} transmitting or indicating the displacement of mechanical pressure-sensitive elements by electric {, e.g., photoelectric} or magnetic means G01L9/00; measuring differences of two or more pressure values G01L13/00; measuring two or more pressure values simultaneously G01L15/00) · CPC title

• G01G19/00

  Weighing apparatus or methods adapted for special purposes not provided for in the preceding groups {(electric measuring arrangements involving comparison with a reference value G01R17/00)} · CPC title