Matrix arrays and methods for making same

Patent metadata

Field	Value
Publication number	US-10738353-B2
Application number	US-201816120007-A
Country	US
Kind code	B2
Filing date	Aug 31, 2018
Priority date	Mar 14, 2013
Publication date	Aug 11, 2020
Grant date	Aug 11, 2020

Abstract

A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

First claim

What is claimed is:

1. A method of forming a polymer matrix array, the method comprising:
flowing an emulsion over applying an aqueous solution into wells of a well array, the emulsion including an aqueous dispersed phase in an immiscible continuous phase, the aqueous dispersed phase including solution comprising polymer precursors, the aqueous dispersed phase coalescing in the wells of the well array, wherein the wells of the well array have a thickness in a range of 0.01 micrometers to 10 micrometers;
applying an immiscible fluid over the well array to isolate the aqueous dispersed phase solution within the wells of the well array; and
polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array.

2. The method of claim 1 , wherein the wells have a characteristic diameter in a range of 0.1 micrometers to 2 micrometers.

3. The method of claim 1 , wherein the wells of the well array overlie sensors of a sensor array, the wells corresponding to ion sensitive material of the sensors of the sensor array.

4. The method of claim 1 , wherein a polymer matrix of the polymer matrix array is conformal with walls of a well of the well array.

5. The method of claim 1 , wherein the polymer precursors include a radically polymerizable monomer.

6. The method of claim 5 , wherein the radically polymerizable monomer includes a vinyl-based monomer.

7. The method of claim 6 , wherein the vinyl-based monomer includes acrylamide, vinyl acetate, hydroxyalkylmethacrylate, variations or derivatives thereof or any combination thereof.

8. The method of claim 1 , wherein the polymer precursors include a crosslinker.

9. The method of claim 8 , wherein the crosslinker is a bis-acrylamide.

10. The method of claim 1 , wherein the polymer precursors include a surface active additive.

11. The method of claim 1 , wherein the polymer precursors include an oligonucleotide functionalized acrylamide.

12. The method of claim 1 , wherein the polymer precursors include N-(5-bromoacetamidylpentyl)acrylamide.

13. The method of claim 1 , further comprising spinning the well array following flowing the emulsion applying the aqueous solution.

14. The method of claim 1 , further comprising sonicating the well array following flowing the emulsion applying the aqueous solution.

15. The method of claim 1 , further comprising degassing the well array during flowing the emulsion applying the aqueous solution.

16. The method of claim 1 , further comprising wetting the well array with a hydrophilic fluid prior to flowing the emulsion applying the aqueous solution.

17. The method of claim 1 , wherein the immiscible fluid includes an initiator.

18. The method of claim 1 , further comprising treating a surface within the well with a surface active agent prior to flowing the emulsion applying the aqueous solution.

Assignees

• Life Technologies Corp

Inventors

• Light David
• Hinz Wolfgang
• Cicero Ronald
• Inman Christina
• Kenney Paul
• Mastroianni Alexander
• Rozhkov Roman
• Wang Yufang
• Gray Jeremy
• Glazer Marc
• Gremyachinskiy Dmitriy

Classifications

• C12Q1/6874

  involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title

• C12Q1/6834 Primary

  Enzymatic or biochemical coupling of nucleic acids to a solid phase · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• C12Q1/6869

  Methods for sequencing · CPC title

• C12Q1/6837 Primary

  using probe arrays or probe chips (C12Q1/6874 takes precedence) · CPC title