Matrix arrays and methods for making same

Patent metadata

Field	Value
Publication number	US-10066260-B2
Application number	US-201414774662-A
Country	US
Kind code	B2
Filing date	Mar 13, 2014
Priority date	Mar 14, 2013
Publication date	Sep 4, 2018
Grant date	Sep 4, 2018

Abstract

A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

First claim

What is claimed is:

1. A method of forming a polymer matrix array, the method comprising:
flowing an aqueous solution over wells of a well array, the aqueous solution comprising polymer precursors, the aqueous solution entering the wells;
displacing the aqueous solution from over the wells by flowing an immiscible fluid over the well array to isolate the aqueous solution comprising the polymer precursors within the wells of the well array; and
polymerizing the polymer precursors in the aqueous solution isolated in the wells of the well array by the immiscible fluid to form the polymer matrix array wherein the wells of the well array overlie sensors of a sensor array, the wells corresponding to ion sensitive material of the sensors of the sensor array.

2. The method of claim 1 , wherein the well has a characteristic diameter in a range of 0.1 micrometers to 2 micrometers.

3. The method of claim 1 , wherein the well has a thickness in a range of 0.01 micrometers to 10 micrometers.

4. The method of claim 1 , wherein a polymer matrix of the polymer matrix array is conformal with walls of a well of the well array.

5. The method of claim 1 , wherein the polymer precursors include a radically polymerizable monomer.

6. The method of claim 5 , wherein the radically polymerizable monomer includes a vinyl-based monomer.

7. The method of claim 5 , wherein the radically polymerizable monomer includes acrylamide, vinyl acetate, hydroxyalkylmethacrylate, variations or derivatives thereof or any combination thereof.

8. The method of claim 1 , wherein the polymer precursors include a crosslinker.

9. The method of claim 8 , wherein the crosslinker is a bis-acrylamide.

10. The method of claim 1 , wherein the polymer precursors include a surface active additive.

11. The method of claim 1 , wherein the polymer precursors include an oligonucleotide functionalized acrylamide.

12. The method of claim 1 , wherein the polymer precursors include N-(5- bromoacetamidylpentyl)acrylamide.

13. The method of claim 1 , wherein applying the aqueous solution includes applying an emulsion including the aqueous solution as a dispersed phase.

14. The method of claim 1 , further comprising spinning the well array following flowing the aqueous solution.

15. The method of claim 1 , further comprising sonicating the well array following flowing the aqueous solution.

16. The method of claim 1 , further comprising degassing the well array during flowing the aqueous solution.

17. The method of claim 1 , further comprising wetting the well array with a hydrophilic fluid prior to flowing the aqueous solution.

18. The method of claim 1 , wherein the immiscible fluid includes an initiator.

19. The method of claim 1 , further comprising treating a surface within the well with a surface active agent prior to flowing the aqueous solution.

Assignees

• Life Technologies Corp

Inventors

• Light David
• Hinz Wolfgang
• Cicero Ronald L
• Inman Christina E
• Kenney Paul M
• Mastroianni Alexander
• Rozhkov Roman
• Wang Yufang
• Gray Jeremy
• Glazer Marc
• Gremyachinskiy Dmitriy

Classifications

• C12Q1/6874

  involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title

• C12Q2523/32

  Centrifugation · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• C12Q2527/119

  pH · CPC title

• C12Q1/6837 Primary

  using probe arrays or probe chips (C12Q1/6874 takes precedence) · CPC title