Fungal strain Beauveria sp. MTCC 5184 and a process for the preparation of enzymes therefrom
US-9217140-B2 · Dec 22, 2015 · US
Lipase variants and polynucleotides encoding same
US10724019B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10724019-B2 |
| Application number | US-201916540180-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 14, 2019 |
| Priority date | Jul 6, 2015 |
| Publication date | Jul 28, 2020 |
| Grant date | Jul 28, 2020 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 51; 52; 54; 55; 57; 58; 59; 60; 69; 70; 71; 72; 81; 82; 85; 86; 87; 89; 90; 91; 92; 94; 95; 96; 97; 99; 100; 101; 102; 103; 105; 108; 111; 115; 116; 118; 119; 120; 122; 123; 124; 126; 127; 128; 129; 130; 131; 132; 133; 134; 136; 144; 152; 155; 157; 158; 159; 161; 162; 163; 177; 178; 181; 182; 184; 185; 187; 189; 191; 196; 197; 199; 203; 205; 206; 207; 208; 209; 211; 215; 220; 221; 222; 223; 224; 226; 229; 230; 232; 235; 236; 240; 242; 243; 244; 247; 248; 252; 254; 255; 257; 260; 261; 262 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
First claim
Opening claim text (preview).
The invention claimed is: 1. A polynucleotide encoding a lipase variant of a parent lipase, which variant has lipase activity, has at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises two or more of the substitutions selected from: R131A, R131G, R131K, R131 L, R131M, R131S, R131W, S152A, T176N, V247A, V247F, V247K, V247L, V247S, V247T, V247W, D255A, and D255G of SEQ ID NO: 3. 2. The polynucleotide of claim 1 , wherein said lipase variant comprises three, four or five of the substitutions selected from: R131A, R131G, R131K, R131 L, R131M, R131S, R131W, S152A, T176N, V247A, V247F, V247K, V247L, V247S, V247T, V247W, D255A, and D255G of SEQ ID NO: 3. 3. The polynucleotide of claim 1 , wherein said lipase variant has at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises at least one of the substitutions selected from the group consisting of: a) N97D+T176N b) Q49K+D255G c) N97D+D255G d) S152A+D255G e) H158Q+D255G f) S152A+H158Q+K221S g) Q49K+T176N+D255G h) N97D+T176N+D255G i) S152A+T176N+D255G j) K108A+H158Q+K221S k) N97D+H158Q+K221S l) N97D+R131A+D255G m) N97D+V247A+D255G n) T176N+K221S+V247A+D255G o) S152A+T176N+K221S+V247G+D255G p) S152A+T176N+V247N+D255G q) S152A+T176N+I219N+M220D+V247P r) N97D+S152A+T176N+V247P+D255G s) R131A+S152A+T176N+V247P+D255G t) N97D+S152A+T176N+V247F+D255G u) K108A+S152A+T176N+V247F+D255G v) R131A+S152A+T176N+V247F+D255G w) R131A+S152A+T176N+L225V+P229S+V247F+D255G aa) R131A+S152A+I174V+V247F+D255G ab) K70E+R131A+S152A+T176N+V247F+D255G ac) R131A+S152A+T176N+G206P+V247F+D255G ad) L111S+R131A+S152A+T176N+V247F+D255G ae) L111T+R131A+S152A+T176N+V247F+D255G af) V101R+R131A+S152A+T176N+V247F+D255G ag) D130R+S152A+T176N+V247F+D255G ah) N97L+R131A+S152A+T176N+V247F+D255G ai) R131A+S152A+T176N+V247F aj) R131A+S152A+T176N+S223R+V247F ak) R131A+S152A+T176N+1232E+V247F+D255G al) R131A+S152A+T176N+1219N+V247F+D255G am) R131A+S152A+T176N+1219E+V247F+D255G an) N97L+V101R+R131A+S152A+T176N+V247F+D255G ao) L111T+D130R+S152A+T176N+V247F+D255G ap) V101R+R131A+S152A+T176N+V247F+D255G aq) V101R+D130R+S152A+T176N+V247F+D255G ar) R131A+S152A+T176N+A207E+V247F+D255G as) K108V+R131A+S152A+T176N+V247F+D255G at) K70E+D130R+R131A+S152A+T176N+A207E+V247F+D255G au) D130R+R131A+S152A+T176N+S223R+V247F+D255G av) V101R+R131A+S152A+T176N+S223R+L251F aw) V101R+R131A+L144I+S152A+T176N+S223R+V247F+D255G ba) V101R+D130R+R131A+S152A+T176N+S223R+V247F+D255G bb) V101R+R131A+S152A+T176N+S223R+V247F+D255G bc) V101R+R131A+S152A+T176N+G206V+S223R+V247F+D255G bd) V101R+R131A+S152A+T176N+G206S+S223R+V247F+D255G be) K70E+V101R+R131A+S152A+T176N+S223R+V247F+D255G bf) D4C+V101R+R131A+S152A+T176N+S223R+N236C+V247F+D255G bg) V101R+D130R+R131A+S152A+T176N+G206V+S223R+V247F+D255G bh) V101R+R131A+L144I+S152A+T176N+G206V+S223R+V247F+D255G bi) V101R+R131A+S152A+T176N+G206V+S223R+V247F+D255G bj) V101R+R131A+S152A+T176N+G206V+S223R+L251F+D255G bk) D4C+V101R+D130R+R131A+S152A+T176N+G206P+S223R+N236C+V247F+D255G bl) D4C+A45V+V101R+D130R+R131A+S152A+T176N+G206V+S223R+N236C+V247F+D255G bm) D4C+Q49V+V101R+D130R+R131A+S152A+T176N+G206V+S223R+N236C+V247F+D255G bn) D4C+N97D+D130R+R131A+S152A+T176N+G206V+S223R+N236C+V247F+D255G bo) D4C+V101R+R131A+S152A+T176N+G206V+S223R+N236C+V247F+D255G bp) D4C+V101R+D130R+R131A+S152A+T176N+S223R+N236C+V247F+D255G bq) D4C+V101R+R131A+S152A+T176N+S223R+N236C+V247F+D255G. 4. The polynucleotide of claim 1 , wherein said lipase variant has at least 80% but less than 100% sequence identity to SEQ ID NO: 3. 5. The polynucleotide of claim 1 , wherein the number of substitutions are between 1-40. 6. A nucleic acid construct comprising the polynucleotide of claim 1 . 7. An expression vector comprising the polynucleotide of claim 1 . 8. A host cell comprising the polynucleotide of claim 1 . 9. A method of producing a lipase variant, comprising: (a) cultivating the host cell of claim 8 under conditions suitable for expression of the variant; and (b) recovering the variant.
Assignees
Inventors
Classifications
Chemically modified or immobilised enzymes · CPC title
containing lipase · CPC title
- C12N9/20Primary
Triglyceride splitting, e.g. by means of lipase · CPC title
Phospholipase A2 (3.1.1.4) · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US10724019B2 cover?
- The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 13; 14; 15; 17; 18; 29; 30; 33; 34; 36; 37; 38; 40; 41; 43; 44; 45; 46; 47; 49; 50; 51; 52; 54; 55; 57; 58; 5…
- Who is the assignee on this patent?
- Novozymes As
- What technology area does this patent fall under?
- Primary CPC classification C12N9/20. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 28 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).