High-resolution optical molecular imaging systems, compositions, and methods

Patent metadata

Field	Value
Publication number	US-10716867-B2
Application number	US-201615548151-A
Country	US
Kind code	B2
Filing date	Feb 5, 2016
Priority date	Feb 6, 2015
Publication date	Jul 21, 2020
Grant date	Jul 21, 2020

Abstract

A composition includes a plurality of gold nanoparticles each having at least one surface. The gold nanoparticles have an average length of at least about 90 nm and an average width of at least about 25 nm.

First claim

What is claimed is:

1. A composition comprising:
a plurality of gold nanorods within an aqueous solution, the plurality of gold nanorods prepared for in vivo optical coherence tomography imaging of a target tissue,
each of the plurality of gold nanorods having at least one surface,
the plurality of gold nanorods having an average length of between about 90 nm and about 150 nm and an average width of between about 25 nm and about 50 nm,
each of the plurality of gold nanorods being coated with polystyrene sulfonate (PSS),
the polystyrene sulfonate coating configured to maintain nonaggregation of the plurality of gold nanorods during the in vivo optical coherence tomography imaging of the target tissue.

2. The composition of claim 1 , wherein the average length is about 90 nm and the average width is about 30 nm.

3. The composition of claim 1 , wherein the plurality of gold nanorods is within the aqueous solution as a colloidal suspension.

4. The composition of claim 1 , wherein the PSS is electrostatically adsorbed on the at least one surface of the plurality of gold nanorods.

5. The composition of claim 4 , further comprising:
one or more functional groups associated with the PSS.

6. The composition of claim 5 , wherein the one or more functional groups are covalently associated with the PSS.

7. The composition of claim 5 , wherein the one or more functional groups are electrostatically associated with the PSS.

8. The composition of claim 5 , wherein the one or more functional groups are associated with the PSS via one or more thiol groups.

9. The composition of claim 5 , wherein the one or more functional group includes a biotin.

10. The composition of any one of claim 5 , wherein the one or more functional groups includes any of an avidin, a poly(ethylene) glycol (PEG), an antibody, or an antigen binding portion of the antibody.

11. The composition of claim 9 , wherein the biotin is PEGylated biotin.

12. The composition of claim 5 , wherein the one or more functional group comprises a biotinylated ligand.

13. The composition of claim 12 , wherein the biotinylated ligand is at least one of a biotinylated antibody, a fragment of the biotinylated antibody, a biotinylated antibody-drug conjugate, a biotinylated peptide, a biotinylated enzyme, a biotinylated G protein coupled receptor (GPCR) ligand, or a biotinylated transmembrane receptor ligand.

14. The composition of claim 4 , further comprising:
a plurality of polystyrene beads conjugated to at least a portion of the plurality of gold nanorods.

15. A method of imaging of a target tissue, comprising:
administering, to the target tissue, a composition including a plurality of gold nanorods each having at least one surface, at least one of the gold nanorods of the plurality of gold nanorods including an anionic polymer electrostatically adsorbed on the at least one surface of the gold nanorod, the anionic polymer being polystyrene sulfonate (PSS), the plurality of gold nanorods having an average length of between about 90 nm and about 150 nm and an average width of between about 25 nm and about 50 nm;
imaging, via optical coherence tomography imaging, the target tissue using an imaging apparatus to acquire an interference spectrum;
generating, based on the interference spectrum, a first spectral band and a second spectral band, the second spectral band different from the first spectral band;
generating a first image based on the first spectral band;
generating a second image based on the second spectral band;
generating difference information by subtracting one of the first image and the second image from the other of the first image and the second image; and
transmitting an indication of the difference information.

16. The method of claim 15 , wherein the plurality of gold nanorods is present as a colloidal suspension.

17. A composition comprising:
a plurality of gold nanorods each having at least one surface, at least one of the gold nanorods of the plurality of gold nanorods including polystyrene sulfonate (PSS) electrostatically adsorbed on the at least one surface of the gold nanorod, the plurality of gold nanorods prepared for in vivo imaging of a target tissue,
the plurality of gold nanorods having an average length of between about 90 nm and about 150 nm and an average width of between about 25 nm and about 50 nm,
the polystyrene sulfonate (PSS) coating configured to maintain nonaggregation of the plurality of gold nanorods during the in vivo imaging of the target tissue to maintain an enhanced optical performance produced by the length and width of the plurality of gold nanorods.

18. The composition of claim 1 , wherein an average aspect ratio of the plurality of gold nanorods is about 3.

19. The method of claim 15 , wherein a majority of the plurality of gold nanorods include the anionic polymer electrostatically adsorbed on the at least one surface, the majority of the plurality of gold nanorods including one or more functional groups associated with the anionic polymer.

20. The method of claim 19 , wherein the one or more functional group includes any of a biotin, an avidin, a poly(ethylene) glycol (PEG), an antibody, or an antigen binding portion of the antibody.

21. The composition of claim 17 , wherein an average aspect ratio of the plurality of gold nanorods is about 3.

22. The composition of claim 17 , wherein a majority of the plurality of gold nanorods include the PSS electrostatically adsorbed on the at least one surface, the majority of the plurality of gold nanorods including one or more functional groups associated with the PSS.

23. The composition of claim 22 , wherein the one or more functional group includes any of a biotin, an avidin, a poly(ethylene) glycol (PEG), an antibody, or an antigen binding portion of the antibody.

Assignees

• Univ Leland Stanford Junior

Inventors

• De La Zerda Adam
• Liba Orly
• Sorelle Elliott
• Knysh Bryan

Classifications

• A61K49/0065 Primary

  the luminescent/fluorescent agent having itself a special physical form, e.g. gold nanoparticle · CPC title

• A61B5/0066

  Optical coherence imaging · CPC title

• A61B5/0084

  for introduction into the body, e.g. by catheters (A61B5/1459 takes precedence) · CPC title

• A61K49/0093 Primary

  Nanoparticle, nanocapsule, nanobubble, nanosphere, nanobead, i.e. having a size or diameter smaller than 1 micrometer, e.g. polymeric nanoparticle · CPC title

• C01G7/00

  Compounds of gold · CPC title