Smartphone-based apparatus and method for obtaining repeatable, quantitative colorimetric measurement

Patent metadata

Field	Value
Publication number	US-9445749-B2
Application number	US-201414762214-A
Country	US
Kind code	B2
Filing date	Jan 21, 2014
Priority date	Jan 21, 2013
Publication date	Sep 20, 2016
Grant date	Sep 20, 2016

Abstract

A method for obtaining a point-of-collection, selected quantitative indicia of an analyte on a test strip using a smartphone involves imaging a test strip on which a colorimetric reaction of a target sample has occurred due to test strip illumination by the smartphone. The smartphone includes a smartphone app and a smartphone accessory that provides an external environment-independent/internal light-free, imaging environment independent of the smartphone platform being used. The result can then be presented quantitatively or turned into a more consumer-friendly measurement (positive, negative, above average, etc.), displayed to the user, stored for later use, and communicated to a location where practitioners can provide additional review. Additionally, social media integration can allow for device results to be broadcast to specific audiences, to compare healthy living with others, to compete in health based games, create mappings, and other applications.

First claim

We claim:

1. A method for obtaining a point-of-collection, selected quantitative indicia of an analyte on a test platform, comprising:
providing a modular, colorimetric reactive test platform having a test region and a calibration region;
providing an analyte to be tested on the test region of the modular, colorimetric test platform, wherein the test region is adapted to enable a colorimetric reaction to the analyte;
obtaining a color image of the test region containing the analyte and the calibration region;
selecting an array of pixels in each of the color images of the test region containing the analyte and the calibration region;
determining a median RGBA color value for each of the arrays of pixels;
converting the median RGBA color value for each of the arrays of pixels to a respective Hue-Saturation-Luminosity (HSL or HSV) test color space value and a HSL or HSV calibration color space value;
providing a calibration indicia that relates a selected quantitative indicia of the analyte to a characteristic of the HSL or HSV calibration color space value; and
associating a median HSL or HSV test color space value with the HSL or HSV calibration color space value to determine the selected quantitative indicia of the analyte.

2. The method of claim 1 , wherein the colorimetric reactive test platform is sensitive to at least one of a chemical colorimetric reaction, an enzymatic colorimetric reaction, and a gold nanoparticle colorimetric reaction.

3. The method of claim 1 , wherein the modular, colorimetric test platform is a disposable test strip.

4. The method of claim 1 , wherein the indicia of the analyte is one of pH, cholesterol, and vitamin D.

5. The method of claim 1 , wherein the calibration region maintains a constant color in the presence of a varying amount of the selected indicia of the analyte.

6. The method of claim 5 , wherein the calibration region includes a plurality of calibration regions each of which has a different calibration color.

7. The method of claim 1 , wherein the calibration indicia is a calibration curve that relates the selected quantitative indicia of the analyte to a hue value of the HSL or HSV calibration color space value.

8. The method of claim 1 , comprising obtaining the color image of the test region containing the analyte and the calibration region using a smartphone including a light source and an image detector.

9. The method of claim 8 , further comprising displaying the determined selected quantitative indicia of the analyte on the smartphone.

10. The method of claim 8 , further comprising providing a smartphone accessory that can be removeably coupled to the smartphone, wherein the smartphone accessory is adapted to receive the modular, colorimetric test platform, further wherein at least one of the modular, colorimetric test platform and the smartphone accessory includes a light diffuser and/or a light-diffusing pathway so as to ensure a uniform and repeatable illumination of at least a desired region of the modular, colorimetric test platform, further wherein the smartphone accessory is substantially light-tight when the test platform is disposed therein, so as to enable consistent internal illumination conditions independently of any external conditions.

11. The method of claim 10 , wherein the light source is one of an internal smartphone flash source and an external LED source.

12. The method of claim 10 , wherein obtaining a color image of the test region containing the analyte and the calibration region further comprises illuminating a rear surface of the test strip that is facing the light source with diffused light from the light source.

13. The method of claim 8 , further comprising time stamping the determined selected quantitative indicia of the analyte and storing the determined value for future access.

14. The method of claim 8 , further comprising location stamping the determined selected quantitative indicia of the analyte and storing the determined value for future access.

15. The method of claim 13 or claim 14 , further comprising determining a temporal and/or a location trend of a plurality of the determined selected quantitative indicia of the analyte.

16. The method of claim 13 or claim 14 , comprising storing the time and/or location data in at least one of a readable file in the smartphone, an external readable file, and in a Cloud file.

17. The method of claim 8 , further comprising correlating the determined selected quantitative indicia of the analyte to a related selected metric and displaying a value of the related selected metric on the smartphone.

18. The method of claim 8 , comprising using a brand-independent or operating-system-independent smartphone.

19. The method of claim 1 , wherein the analyte is one of sweat, saliva, blood, tears, urine, and other bodily fluids.

20. The method of claim 1 , wherein the step of obtaining a color image of the test region containing the analyte and the calibration region comprises illuminating a rear surface of the modular, colorimetric test platform.

21. A portable, modular, point-of-collection, colorimetric-based diagnostic system, comprising:
a smartphone including an image detector;
a smartphone accessory including:
a housing that can be removeably attached to the smartphone in a manner that at least optically couples the smartphone accessory to a resident smartphone camera, wherein the housing is opaque such that the smartphone accessory is substantially externally light-tight when a test strip is disposed therein, further wherein the housing includes at least one of a designed-in optical pathway and a light diffuser in the housing for providing diffuse illumination of a surface of the test strip disposed therein from an internal light source resident in the housing or an external light source resident in the smartphone to which the smartphone accessory can be attached; and

an executable application resident in the smartphone that, in operation, performs the following steps:
acquires an image of at least a portion of the test strip;
stores the image as an RGBA byte array;
splits the image into a test image and a calibration image;
for the calibration image: extracts a calibration array of pixels;
determines a median RGBA color value for the calibration array of pixels;
converts the median RGBA color value for the calibration array of pixels to a calibration Hue-Saturation-Luminosity (HSL or HSV) color space value;
adjusts the calibration HSL or HSV color space value to a calibration indicia of a selected quantitative indicia of an analyte to be measured; and
for the test image: extracts a test array of pixels;
determines a median RGBA color value for the test array of pixels;
associates the median RGBA color value for the test array of pixels to the calibration HSL or HSV color space value; and

determines a quantitative value of the selected indicia of the analyte to be measured.

22. The system of claim 21 , wherein the light source is an internal flash source of the smartphone.

23. The system of claim 21 , wherein the light source is an LED disposed in the smartphone accessory, further comprising a battery in the smartphone accessory to power the LED.

24. The system of claim 21 , wherein the system is smartphone platform-independent.

25. The system of claim 21 , wherein the smartphone accessory is an unpowered component.

26. The system of claim 25 ,

Assignees

• Univ Cornell

Inventors

• Erickson David
• O'Dell Dakota
• Oncescu Vlad-Victor
• Mancuso Matthew
• Lee Seoho

Classifications

• G16H40/67

  for remote operation · CPC title

• A61B5/150358

  Strips for collecting blood, e.g. absorbent · CPC title

• A61B5/0022

  Monitoring a patient using a global network, e.g. telephone networks, internet · CPC title

• A61B5/150022

  for capillary blood or interstitial fluid · CPC title

• G01N21/80

  Indicating pH value · CPC title