Gas permeable material

Patent metadata

Field	Value
Publication number	US-10711235-B2
Application number	US-201815937434-A
Country	US
Kind code	B2
Filing date	Mar 27, 2018
Priority date	Dec 22, 2014
Publication date	Jul 14, 2020
Grant date	Jul 14, 2020

Abstract

The invention describes a gas permeable fluoropolymer and silicone material used in the construction of cell culture bags.

First claim

The invention claimed is:

1. An oxygen permeable film comprising:
a first polymer layer having a thickness in the range of about 0.01 mm to about 5 mm; and
a second fluid contact layer having a total organic carbon (TOC) in water of less than 1 mg/cm 2 , the second fluid contact layer having a thickness in the range of about 0.01 mm to about 0.1 mm,
wherein the first polymer layer and the second fluid contact layer are adhered together, and
wherein the oxygen permeable film has an oxygen permeability of at least 2000 cc/m 2 per day.

2. The film of claim 1 , wherein the first polymer layer is a first elastomer layer.

3. The film of claim 1 , wherein the first polymer layer and the second fluid contact layer are adhered together by bonding.

4. The film of claim 3 , wherein the bonding is by chemical bonding, thermal fusion bonding, solvent bonding, laser welding, surface treatment, extrusion, co-extrusion, lamination, or combinations thereof.

5. The film of claim 1 , wherein the TOC in water of the second fluid contact layer is less than 0.1 mg/cm 2 .

6. The film of claim 1 , wherein the TOC in water of the second fluid contact layer is less than 0.01 mg/cm 2 .

7. An oxygen permeable container comprising:
an outer polymer layer having a thickness in the range of about 0.01 mm to about 5 mm; and
an inner fluid contact layer having a total organic carbon (TOC) in water of less than 1 mg/cm 2 , the inner fluid contact layer having a thickness in the range of about 0.01 mm to about 0.1 mm,
wherein the outer polymer layer and the inner fluid contact layer are adhered together, and
wherein the oxygen permeable film has an oxygen permeability of at least 2000 cc/m 2 per day.

8. The container of claim 7 , wherein the outer polymer layer is an outer elastomer layer.

9. The container of claim 7 , wherein the container comprises a bag, flask, or tube.

10. The container of claim 7 , wherein the container is in the form of a cell culture bag comprising an oxygen permeable film.

11. The container of claim 7 , wherein the container comprises at least one sealable access port.

12. The container of claim 11 , wherein the access port comprises a sealable lid, a screw cap, a puncturable material, a puncturable septa, or a valve assembly.

13. The container of claim 11 , wherein the access port comprises fluoropolymer tubes.

14. The container of claim 13 , wherein the fluoropolymer tubes are attached to the container by RF welding, ultrasonic welding, hot bar welding, chemical bonding, adhesive bonding, thermal fusion bonding, solvent welding, laser welding, corona discharge, radiation, lamination, etching, plasma treatment, wetting, adhesives, radiation, co-extrusion, or combinations thereof.

15. The container of claim 9 , further comprising a sensor to monitor cell culture conditions.

16. The container of claim 9 , wherein the outer polymer layer and the second fluid contact layer are adhered together by bonding.

17. The container of claim 16 , wherein the bonding is by chemical bonding, thermal fusion bonding, solvent bonding, laser welding, surface treatment, extrusion, co-extrusion, lamination, or combinations thereof.

18. The container of claim 9 , wherein the outer polymer layer and the inner fluid contact layer are adhered together by a method comprising at least corona treatment of the inner fluid contact layer in an organic atmosphere.

19. The container of claim 9 , wherein the TOC in water of the inner fluid contact layer is less than 0.1 mg/cm 2 .

20. A method to cultivate cells in an oxygen permeable container, the method comprising:
a) adding media to the container; and
b) adding cells, optionally with media, to the container, wherein the container comprises:
an outer polymer layer having a thickness in the range of about 0.01 mm to about 5 mm; and
an inner fluid contact layer having a total organic carbon (TOC) in water of less than 1 mg/cm 2 , the inner fluid contact layer having a thickness in the range of about 0.01 mm to about 0.1 mm,
wherein the outer polymer layer and inner fluid contact layer are adhered together,
wherein the container is in the form of a cell culture bag, and
wherein the oxygen permeable film has an oxygen permeability of at least 2000 cc/m 2 per day.

Assignees

• Saint Gobain Performance Plastics Corp

Inventors

• Clark Sarah Louise
• Lundgren Natasha Anna
• Tzivanis Michael John
• Zhu Aijun

Classifications

• B32B27/322

  comprising halogenated polyolefins, e.g. PTFE · CPC title

• B32B2597/00

  Tubular articles, e.g. hoses, pipes · CPC title

• B32B25/18

  comprising butyl or halobutyl rubber · CPC title

• B32B25/12

  comprising natural rubber · CPC title

• B32B2307/7265

  Non-permeable · CPC title