Gas permeable material

Patent metadata

Field	Value
Publication number	US-9926524-B2
Application number	US-201514976372-A
Country	US
Kind code	B2
Filing date	Dec 21, 2015
Priority date	Dec 22, 2014
Publication date	Mar 27, 2018
Grant date	Mar 27, 2018

Abstract

The invention describes a gas permeable fluoropolymer and silicone material used in the construction of cell culture bags.

First claim

What is claimed is:

1. An oxygen permeable film comprising:
a first elastomer layer having a thickness in the range of about 0.05 mm to about 1 mm; and
a second fluid contact layer having a total organic carbon (TOC) in water of less than 1 mg/cm 2 , the second fluid contact layer having a thickness in the range of about 0.01 mm to about 0.1 mm,
wherein the first elastomer layer and the second fluid contact layer are adhered together by a method comprising at least corona treatment of the second fluid contact layer in an organic atmosphere, and
wherein the oxygen permeable film has an oxygen permeability of at least 2000 cc/m 2 per day.

2. The film of claim 1 , wherein the second fluid contact layer is a fluoropolymer layer.

3. The film of claim 1 , wherein the first elastomer layer comprises natural polyisoprene rubber (NR), synthetic polyisoprene rubber (IR), polybutadiene rubber (BR), chloroprene rubber (CR), butyl rubber (IIR), halogenated butyl rubbers (CIIR, BIIR), styrene-butadiene rubber (SBR), nitrile rubber (NBR) and hydrogenated nitrile rubber (HNBR), ethylene propylene rubber (EPM), ethylene propylene diene rubber (EPDM), epichlorohydrin rubber (ECO), polyacrylic rubber (ACM, ABR), silicone rubber (SI, Q, VMQ), fluorosilicone rubber (FSR, FVMQ), fluoroelastomers (FKM, FEPM), perfluoroelastomers (FFKM), polyether block amides (PEBA), chlorosulfonated polyethylene (CSM), ethylene-vinyl acetate (EVA), cyclic olefin copolymers, polyolefin elastomers, elastomeric PET, or mixture thereof.

4. The film of claim 1 , wherein the first elastomer layer is polymethylpentene polymer (PMP).

5. The film of claim 1 , wherein the first elastomer layer is silicone rubber.

6. The film of claim 5 , wherein the silicone rubber is high consistency rubber (HCR), fluorosilicone rubber (FSR), liquid silicone rubber (LSR), room temperature vulcanized rubber (RTV), thermoplastic silicone rubber (TPE), platinum cured silicone rubber, or peroxide cured silicone rubber.

7. The film of claim 2 , wherein the second fluoropolymer layer is fluorinated ethylene-propylene (FEP), polytetrafluoroethylene (PTFE), polyvinylidenefluoride (PVDF), tetrafluoroethylene-perfluoro(propyl vinyl ether) (PFA), polyvinylidene difluoride (PVF), polychlorotrifluoroethlylene (PCTFE), tetrafluoroethylene/hexafluoropropylene/ethylene copolymer(HTE), chlorotrifluoroethylene/vinylidenefluoride copolymer, chlorotrifluoroethylene/hexafluoropropylene, ethylene/chlorotrifluoroethylene copolymers (ECTFE), ethylene/trifluoroethylene copolymers, ethylene/tetrafluoroethylene copolymers (ETFE), tetrafluoroethylene/propylene copolymers(TFE/P), tetrafluoroethylene/hexafluoropropylene copolymers (FEP/HFP), hexafluoropropylene/tetrafluoroethylene/vinylidene copolymer (THV), or perfluoro(1-butenyl vinyl ether) homocyclopolymer having functionalized polymer-end groups.

8. An oxygen permeable container, wherein
the oxygen permeable container is in the form of a cell culture bag, the cell culture bag comprising an oxygen permeable film comprising:
an outer elastomer layer having a thickness in the range of about 0.05 mm to about 1 mm; and
an inner fluid contact layer having a total organic carbon (TOC) in water of less than 1 mg/cm 2 , the inner fluid contact layer having a thickness in the range of about 0.01 mm to about 0.1 mm,
wherein the first elastomer layer and the second fluid contact layer are adhered together by a method comprising at least corona treatment of the second fluid contact layer in an organic atmosphere,
wherein the oxygen permeable film has an oxygen permeability of at least 2000 cc/m 2 per day.

9. The film of claim 8 , wherein the second fluid contact layer is a fluoropolymer layer.

10. The container of claim 8 , wherein the outer elastomer layer comprises natural polyisoprene rubber (NR), synthetic polyisoprene rubber (IR), polybutadiene rubber (BR), chloroprene rubber (CR), butyl rubber (IIR), halogenated butyl rubbers (CIIR, BIIR), styrene-butadiene rubber (SBR), nitrile rubber (NBR) and hydrogenated nitrile rubber (HNBR), ethylene propylene rubber (EPM), ethylene propylene diene rubber (EPDM), epichlorohydrin rubber (ECO), polyacrylic rubber (ACM, ABR), silicone rubber (SI, Q, VMQ), fluorosilicone rubber (FSR, FVMQ), fluoroelastomers (FKM, FEPM), perfluoroelastomers (FFKM), polyether block amides (PEBA), chlorosulfonated polyethylene (CSM), ethylene-vinyl acetate (EVA), cyclic olefin copolymers, polyolefin elastomers, elastomeric PET, or mixtures thereof.

11. The container of claim 8 , wherein the outer elastomer layer is polymethylpentene polymer (PMP).

12. The container of claim 8 , wherein the outer elastomer layer is silicone rubber.

13. The container of claim 12 , wherein the silicone rubber is high consistency rubber (HCR), fluorosilicone rubber (FSR), liquid silicone rubber (LSR), or room temperature vulcanized rubber (RTV), thermoplastic silicone rubber (TPE), platinum cured silicone rubber, or peroxide cured silicone rubber.

14. The container of claim 9 , wherein the inner fluoropolymer layer is fluorinated ethylene-propylene (FEP), polytetrafluoroethylene (PTFE), polyvinylidenefluoride (PVDF), tetrafluoroethylene-perfluoro(propyl vinyl ether) (PFA), polyvinylidene difluoride (PVF), polychlorotrifluoroethlylene (PCTFE), tetrafluoroethylene/hexafluoropropylene/ethylene copolymer (HTE), chlorotrifluoroethylene/vinylidenefluoride copolymer, chlorotrifluoroethylene/hexafluoropropylene, ethylene/chlorotrifluoroethylene copolymers (ECTFE), ethylene/trifluoroethylene copolymers, ethylene/tetrafluoroethylene copolymers (ETFE), tetrafluoroethylene/propylene copolymers (TFE/P), tetrafluoroethylene/hexafluoropropylene copolymers (FEP/HFP), hexafluoropropylene/tetrafluoroethylene/vinylidene copolymer (THV), or perfluoro(1-butenyl vinyl ether) homocyclopolymer having functionalized polymer-end groups.

15. The container of claim 8 , wherein the bag contains at least one sealable access port.

16. A method to cultivate cells in an oxygen permeable container comprising the steps of:
a) adding media to the container; and
b) adding cells, optionally with media, to the container, wherein the container comprises;
an outer elastomer layer having a thickness in the range of about 0.05 mm to about 1 mm; and
an inner fluid contact layer having a total organic carbon (TOC) in water of less than 1 mg/cm 2 , the inner fluid contact layer having a thickness in the range of about 0.01 mm to about 0.1 mm,

wherein the outer elastomer layer and inner fluid contact layer are adhered together by a method comprising at least corona treatment of the inner fluid contact layer in an organic atmosphere,
wherein the container is in the form of a cell culture bag, and
wherein the oxygen permeable film has an oxygen permeability of at least 2000 cc/m 2 per day.

17. The method of claim 16 , where the inner fluid contact layer is a fluoropolymer layer.

18. The method of claim 17 , wherein the inner fluoropolymer layer is fluorinated ethylene-propylene (FEP), polytetrafluoroethylene (PTFE), polyvinylidenefluoride (PVDF), tetrafluoroethylene-perfluoro(propyl vinyl ether) (PFA), polyvinylidene difluoride (PVF), polychlorotrifluoroethlylene (PCTFE), tetrafluoroethylene/hexafluoropropylene/ethylene copolymer (HTE), chlorotrifluoroethylene/vinylidenefluoride copolymer, chlorotrifluoroethylene/hexafluoropropylene, ethylene/chlorotrifluoroethylene copolymers (ECTFE), ethylene/trifluoroethylene copolymers, ethylene/tetrafluoroethylene copolymers (ETFE), tetrafluoroethylene/propylene copolymers (TFE/P), tetrafluoroethylene/hexafl

Assignees

• Saint Gobain Performance Plastics Corp

Inventors

• Clark Sarah Louise
• Lundgren Natasha Anna
• Tzivanis Michael John
• Zhu Aijun

Classifications

• C12M23/20 Primary

  Material Coatings (immunocoatings C12M25/00) · CPC title

• C12M23/24 Primary

  Gas permeable parts · CPC title

• C12M23/26

  flexible (flexible containers for laboratory use B01L3/505) · CPC title

• B32B27/322

  comprising halogenated polyolefins, e.g. PTFE · CPC title

• B32B2597/00

  Tubular articles, e.g. hoses, pipes · CPC title