Sensing platform for transduction of information

Patent metadata

Field	Value
Publication number	US-10684247-B2
Application number	US-201816122346-A
Country	US
Kind code	B2
Filing date	Sep 5, 2018
Priority date	Aug 9, 2013
Publication date	Jun 16, 2020
Grant date	Jun 16, 2020

Abstract

Aspects of a biosensor platform system and method are described. In one embodiment, the biosensor platform system includes a fluidic system and tunneling biosensor interface coupled to the fluidic system. The tunneling biosensor interface may include a transducing electrode array having at least one dielectric thin film deposited on an electrode array. The biosensor platform system may further include processing logic operatively coupled to the transducing electrode array. In operation, the application of an electromagnetic field at an interface between an electrode and an electrolyte in the system, for example, may result in the transfer of charge across the interface. The transfer of charge is, in turn, characterized by electromagnetic field-mediated tunneling of electrons that may be assisted by exchange of energy with thermal vibrations at the interface. Various analytes, for example, and other compositions can be identified by analysis of the transfer of charge.

First claim

At least the following is claimed:

1. A sensor comprising:
a biosensor interface including an electrode and a dielectric film deposited on the electrode, wherein the biosensor interface is configured to operatively couple with a sample comprising a redox specie and an analyte specie; and
a processing logic operatively coupled to the electrode, and configured to apply a voltage bias between the sample and the electrode, the applied voltage bias configured to generate a tunneling current configured to flow between the redox specie and the electrode via the dielectric film,
wherein the tunneling current is indicative of the analyte specie.

2. The sensor of claim 1 , further comprising a probe including a tip at a distal end of the probe, wherein the biosensor interface is coupled to the tip of the probe.

3. The sensor of claim 2 , further comprising:
a second biosensor interface including a second electrode and a second dielectric film deposited on the second electrode, wherein the second biosensor interface is configured to operatively couple with the sample, wherein
the processing logic is operatively coupled to the second electrode, and configured to apply a second voltage bias between the sample and the second electrode, the applied second voltage bias configured to generate a second tunneling current configured to flow from the redox specie to the second electrode via the second dielectric film,
wherein the second tunneling current is indicative of the analyte specie.

4. The sensor of claim 3 , further comprising a second probe including a tip at a distal end of the second probe, wherein the second biosensor interface is coupled to the tip of the second probe.

5. The sensor of claim 2 , further comprising a fluidic system enclosing a volume configured to receive the sample.

6. The sensor of claim 5 , wherein the enclosed volume of the fluidic system is configured to receive the distal end of the probe, wherein the biosensor interface coupled to the tip of the probe is configured to contact a volume of the sample in the fluidic system.

7. The sensor of claim 5 , wherein the tunneling current is indicative of the analyte specie in the sample volume.

8. The sensor of claim 2 , wherein the tip is an insulator.

9. The sensor of claim 1 , wherein the dielectric film comprises a sequential layering of low-k and high-k dielectric materials.

10. The sensor of claim 9 , wherein a layer of low-k material and a layer of high-k material are separated by a layer of non-magnetic dielectric insulator.

11. The sensor of claim 9 , wherein the high-k dielectric materials includes one or more of Ta2O2, ZrO2 and TiO2.

12. The sensor of claim 1 , wherein the processing logic is coupled to the electrode by through-silicon vias.

13. The sensor of claim 1 , wherein the processing logic includes a voltage source configured to apply the voltage bias, and the applied voltage bias is further configured to produce a weakly-coupled non-adiabatic electron flux across the dielectric film.

14. The sensor of claim 1 , wherein the processing logic includes a low noise transimpedance amplifier configured to detect the tunneling current.

15. The sensor of claim 14 , wherein the detected tunneling current is indicative of molecular vibrational states of the analyte specie located at the biosensor interface.

16. A method comprising:
detecting, by a sensor, a signature tunneling current indicative of one or more vibrational states of a first analyte in a sample comprising a redox specie and the first analyte, wherein the sensor is configured to apply a voltage bias between the sample and the sensor, and generate the signature tunneling current between the redox specie and the sensor;
comparing data characterizing the detected signature tunneling current with data characterizing a plurality of signature tunneling currents associated with one or more analytes; and
determining an identity of the first analyte based on the comparison.

17. The method of claim 16 , wherein the sensor includes:
a biosensor interface including an electrode and a dielectric film deposited on the electrode, wherein the biosensor interface is configured to operatively couple with a first portion of the sample; and
a processing logic operatively coupled to the electrode, and configured to apply the voltage bias between the first portion of the sample and the electrode.

18. The method of claim 17 , wherein detecting the signature tunneling current further comprises:
filtering the sample to separate the first portion of the sample from a second portion of the sample, the first portion includes the first analyte and a second analyte;
passing the first portion of the sample through an immunoseparation membrane; and
directing the first analyte and the second analyte to the sensor, wherein the first analyte arrives at the sensor during a first time duration and the second analyte arrives at the sensor during a second time duration.

19. The method of claim 18 , further comprising detecting by the sensor, a second tunneling current indicative of the second analyte during the second time duration.

20. The method of claim 18 , wherein the immunoseparation membrane includes surface antibodies.

21. The method of claim 18 , wherein the immunoseparation membrane includes a nitrocellulose membrane.

22. The method of claim 18 , wherein the first analyte is separated from the second analyte based on charge-to-mass ratio of the first analyte relative to the second analyte.

Assignees

• Univ Leland Stanford Junior
• The Board Of Trustees Of The Leland Stanford Junio

Inventors

• Gupta Chaitanya

Classifications

• B01L3/5027

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• G01N27/3278 Primary

  involving nanosized elements, e.g. nanogaps or nanoparticles (nanopores G01N33/48721; magnetic beads G01N27/745) · CPC title