Culture system and culture method

Patent metadata

Field	Value
Publication number	US-10676707-B2
Application number	US-201414916764-A
Country	US
Kind code	B2
Filing date	Sep 2, 2014
Priority date	Sep 12, 2013
Publication date	Jun 9, 2020
Grant date	Jun 9, 2020

Abstract

A culture system comprises: a preparatory culture vessel and a main culture vessel that accommodate cells and a solution; a main stage that holds the preparatory culture vessel and the main culture vessel; a connecting tube that connects the culture vessels; a valve that opens and closes the connecting tube; and a rotating mechanism that rotates the main stage and imparts a height difference between the culture vessels to transfer the cells and solution by dropping between the culture vessels.

First claim

The invention claimed is:

1. A culture system comprising:
a plurality of housing vessels accommodating cells and a solution;
conduits connecting the plurality of housing vessels;
a valve opening and closing the conduits;
a first holding section for holding the plurality of housing vessels;
an agitator imparting a height difference between the plurality of housing vessels in order to allow dropping of the cells and the solution between the plurality of housing vessels,
magnetic particles that are attached to the cells in the solution,
a plurality of magnets provided outside at least one of the plurality of housing vessels, and
a magnetic force regulating unit regulating magnetic forces of the plurality of magnets
wherein the agitator periodically tilts at least one of the plurality of housing vessels to agitate the cells, and the culture solution in the housing vessels when undergoing culture, and
wherein the magnetic force regulating unit has a flat portion on which the plurality of magnets are arranged in a matrix, at least one of the housing vessels has a flat portion, and the flat portion of the magnetic force regulating unit faces the flat portion of at least one of the housing vessels: and
wherein the agitator has a rotation shaft for rotating the first holding section, the plurality of housing vessels are arranged on the first holding section along a direction intersecting with an axial direction of the rotation shaft.

2. The culture system according to claim 1 , wherein the agitator imparts a height difference to at least one of the plurality of housing vessels by moving the first holding section.

3. The culture system according to claim 1 , wherein the plurality of housing vessels comprises one or a plurality of culture vessels.

4. The culture system according to claim 1 , wherein at least one of the plurality of housing vessels is connected to at least one cylinder or bellows vessel.

5. The culture system according to claim 4 , wherein the agitator imparts a height difference between at least one of the plurality of the housing vessels and the cylinder or the bellows vessel so as to transfer the cells and the solution between at least one of the plurality of housing vessels and the cylinder or the bellows vessel by dropping.

6. The culture system according to claim 5 , wherein the cylinder or the bellows vessel is a waste fluid vessel, and wherein one of the conduits connects between the waste fluid vessel and the at least one of the plurality of the housing vessels, and wherein the one of the conduits is provided with a cell capturing unit.

7. The culture system according to claim 6 , wherein the cylinder or the bellows vessel is a feed fluid vessel, which is connected to the cell capturing unit.

8. The culture system according to claim 7 , comprising a switching mechanism switching between the connection between the cell capturing unit and the cylinder or the bellows vessel.

9. The culture system according to claim 4 , comprising an actuator stretching the cylinder or the bellows vessel.

10. The culture system according to claim 4 , comprising a second holding section for holding the cylinder or the bellows vessel.

11. The culture system according to claim 10 , wherein the first holding section and the second holding section are detachable.

12. The culture system according to claim 4 , wherein the bellows vessel is disposable.

13. The culture system according to claim 1 , wherein the housing vessels are provided with a temperature regulating unit.

14. The culture system according to claim 1 , wherein the housing vessels are provided with a carbon dioxide supply section.

15. The culture system according to claim 1 , wherein the housing vessels are provided with a ventilation section.

16. The culture system according to claim 1 , wherein the housing vessels are disposable.

17. The culture system according to claim 1 , wherein the agitator also swings the housing vessels.

18. The culture system according to claim 1 , further comprising:
wherein the plurality of housing vessels include a culture vessel,
wherein one of the plurality of magnets is provided outside the culture vessel, and
wherein the magnetic force regulating unit regulates a magnetic force of the one of the plurality of magnets provided outside the culture vessel to shake or vibrate the magnetic particles and the cells in the culture vessel.

19. The culture system according to claim 1 , further comprising a controller controlling the valve and the agitator,
wherein the controller controls the valve and the agitator according to a predetermined procedure to perform cell culture and transfer the solution in an automatic manner.

20. A method for performing cell culture, comprising:
accommodating cells and a solution by a plurality of housing vessels;
connecting the plurality of housing vessels by conduits;
opening and closing the conduits by a valve;
holding the plurality of housing vessels by a first holding section;
imparting, by an agitator, a height difference between the plurality of housing vessels in order to allow dropping of the cells and the solution between the plurality of housing vessels;
attaching magnetic particles to the cells in the solution;
providing a plurality of magnets outside at least one of the housing vessels;
regulating, by a magnetic force regulating unit, magnetic forces of the plurality of magnets;
periodically tilting, by the agitator, at least one of the plurality of housing vessels to agitate the cells; and the culture solution in the housing vessels when undergoing culture,
wherein the magnetic force regulating unit has a flat portion on which the plurality of magnets are arranged in a matrix, at least one of the housing vessels has a flat portion, and the flat portion of the magnetic force regulating unit faces the flat portion of at least one of the housing vessels,
wherein the agitator has a rotation shaft for rotating the first holding section, the plurality of housing vessels are arranged on the first holding section along a direction intersecting with an axial direction of the rotation shaft.

21. The method according to claim 20 , comprising the steps of:
culturing at a position where the height difference between the plurality of housing vessels is made smaller by the agitator;
transferring the cells and the solution at a dropping position where the height difference between the plurality of housing vessels is made larger by the agitator; and
treating the solution using at least one cylinder or bellows vessel connected to at least one of the plurality of housing vessels.

22. The method according to claim 21 , wherein the step of treating the solution is a step of discarding the solution or a step of supplying the solution.

Assignees

• Universal Bio Research Co Ltd

Inventors

• Tajima Hideji

Classifications

• C12M23/00 Primary

  Constructional details, e.g. recesses, hinges (flow directing inserts in C12M27/18-C12M27/24; apparatus for chemical or physical processes in general B01J, chemical or physical laboratory apparatus in general B01L) · CPC title

• C12M23/34

  Internal compartments or partitions · CPC title

• C12M47/02 Primary

  Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02) · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M23/28

  disposable or single use · CPC title