Mutant strain of Lactococcus lactis and its application

US10638771B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-10638771-B2
Application numberUS-201715640903-A
CountryUS
Kind codeB2
Filing dateJul 3, 2017
Priority dateAug 10, 2016
Publication dateMay 5, 2020
Grant dateMay 5, 2020

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  1. Title

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  2. Abstract

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  3. Assignees and inventors

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  4. Key dates

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  5. First independent claim

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  7. Citations and related patents

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Abstract

Official abstract text for this publication.

The present invention relates to a mutant strain of Lactococcus lactis and its application, and belongs to the field of food biotechnology. The mutant strain, Lactococcus lactis WH101, was screened for its better tolerance to harsh environmental factors, especially higher acid tolerance. The OD600 of Lactococcus lactis WH101 was increased by 5.5 times than that of the parent strain when cultured at pH 4.5. The survival rate of the mutant strain was 22.4 times higher than that of the parent strain after 3 hr treatment in pH 4.0 culture medium. The survival rate of the mutant strain was 5.2, 2.0 and 1.9 times over that of the parent strain treated with 15% ethanol for 4 hr, 15% NaCl for 6 hr and 1 mM H2O2 for 3 hr, respectively.

First claim

Opening claim text (preview).

What is claimed is: 1. A mutant strain, named Lactococcus lactis WH101, with higher acid tolerance than that of its parent strain, which was deposited in the Chinese Type Culture Collection with the accession CCTCC NO: M 2016233. 2. The mutant strain of claim 1 , wherein said mutant strain exhibits higher acid, ethanol, oxygen and osmotic pressure tolerance than that of its parent strain. 3. A method of for producing a fermentation food, comprising using the mutant strain of claim 1 as the fermentation strain to produce said fermentation food. 4. The method of claim 3 wherein said fermentation food is kimchi. 5. The method of claim 3 wherein said fermentation food is yoghurt.

Assignees

Inventors

Classifications

  • A23C9/123Primary

    using only microorganisms of the genus lactobacteriaceae; Yoghurt (A23C9/13 takes precedence) · CPC title

  • by pickling, e.g. sauerkraut or pickles · CPC title

  • Leaf vegetables, e.g. sauerkraut · CPC title

  • Microorganisms; Enzymes {; Antibiotics} · CPC title

  • Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title

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What does patent US10638771B2 cover?
The present invention relates to a mutant strain of Lactococcus lactis and its application, and belongs to the field of food biotechnology. The mutant strain, Lactococcus lactis WH101, was screened for its better tolerance to harsh environmental factors, especially higher acid tolerance. The OD600 of Lactococcus lactis WH101 was increased by 5.5 times than that of the parent strain when culture…
Who is the assignee on this patent?
Zhang Juan, Zhu Zhengming, Chen Jian, and 3 more
What technology area does this patent fall under?
Primary CPC classification A23C9/123. Mapped technology areas include Human Necessities.
When was this patent published?
Publication date Tue May 05 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).