Milk powder composition
US-2024074447-A1 · Mar 7, 2024 · US
US10638771B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10638771-B2 |
| Application number | US-201715640903-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 3, 2017 |
| Priority date | Aug 10, 2016 |
| Publication date | May 5, 2020 |
| Grant date | May 5, 2020 |
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The present invention relates to a mutant strain of Lactococcus lactis and its application, and belongs to the field of food biotechnology. The mutant strain, Lactococcus lactis WH101, was screened for its better tolerance to harsh environmental factors, especially higher acid tolerance. The OD600 of Lactococcus lactis WH101 was increased by 5.5 times than that of the parent strain when cultured at pH 4.5. The survival rate of the mutant strain was 22.4 times higher than that of the parent strain after 3 hr treatment in pH 4.0 culture medium. The survival rate of the mutant strain was 5.2, 2.0 and 1.9 times over that of the parent strain treated with 15% ethanol for 4 hr, 15% NaCl for 6 hr and 1 mM H2O2 for 3 hr, respectively.
Opening claim text (preview).
What is claimed is: 1. A mutant strain, named Lactococcus lactis WH101, with higher acid tolerance than that of its parent strain, which was deposited in the Chinese Type Culture Collection with the accession CCTCC NO: M 2016233. 2. The mutant strain of claim 1 , wherein said mutant strain exhibits higher acid, ethanol, oxygen and osmotic pressure tolerance than that of its parent strain. 3. A method of for producing a fermentation food, comprising using the mutant strain of claim 1 as the fermentation strain to produce said fermentation food. 4. The method of claim 3 wherein said fermentation food is kimchi. 5. The method of claim 3 wherein said fermentation food is yoghurt.
using only microorganisms of the genus lactobacteriaceae; Yoghurt (A23C9/13 takes precedence) · CPC title
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Leaf vegetables, e.g. sauerkraut · CPC title
Microorganisms; Enzymes {; Antibiotics} · CPC title
Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title
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