Method for imaging biologic fluid samples using a predetermined distribution

Patent metadata

Field	Value
Publication number	US-10627390-B2
Application number	US-201816102036-A
Country	US
Kind code	B2
Filing date	Aug 13, 2018
Priority date	Dec 6, 2012
Publication date	Apr 21, 2020
Grant date	Apr 21, 2020

Abstract

A method for analyzing a biologic fluid sample includes the steps of: a) providing a spatially mapped chamber; b) providing a predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample, which distribution indicates the presence or absence of a statistically significant number of constituents within the sample in each chamber sub-region; c) selecting one or more image techniques for each sub-region based on the presence or absence of the statistically significant number of one or more constituents in that sub-region as indicated by the distribution; d) creating image data representative of the biologic fluid sample in each sub-region, using the one or more image techniques selected for that sub-region; and e) analyzing the sample.

First claim

What is claimed is:

1. A method for imaging a biologic fluid sample, comprising:
providing a stored predetermined distribution of at least one biologic fluid sample constituent type within defined sub-regions of the analysis chamber, the distribution defining:
a first number of said chamber sub-regions within the analysis chamber where the at least one biologic fluid sample constituent is present within a biologic fluid sample quiescently residing within an analysis chamber; and
a second number of said chamber sub-regions within the analysis chamber, wherein the second number of said chamber sub-regions are separate from the first number of said chamber sub-regions; and

controlling an imaging device utilizing the distribution to selectively image the first number of said chamber sub-regions within the analysis chamber.

2. The method of claim 1 , further comprising creating image data representative of the biologic fluid sample residing in the first number of said chamber sub-regions using a first imaging method.

3. The method of claim 2 , further comprising creating image data representative of the biologic fluid sample residing in the second number of said chamber sub-regions using a second imaging method, wherein the first imaging method is different than the second imaging method.

4. The method of claim 1 , wherein the biologic fluid sample is blood and the constituents include at least one of white blood cells, red blood cells, plasma, or sphered red blood cells.

5. The method of claim 4 , wherein the at least one biologic fluid sample constituent within the first number of said chamber sub-regions within the predetermined are white blood cells.

6. The method of claim 4 , wherein the at least one biologic fluid sample constituent within the first number of said chamber sub-regions within the predetermined are red blood cells.

7. The method of claim 1 , wherein the predetermined distribution representative of the at least one constituent within the biologic fluid sample quiescently residing within the analysis chamber is based on empirical data.

8. The method of claim 1 , wherein the chamber is a microscope slide.

9. An apparatus for imaging a biologic fluid sample, the apparatus comprising:
at least one sample illuminator;
at least one image dissector; and
at least one processor in communication with the at least one sample illuminator and the at least one image dissector, the processor in communication with stored instructions, which instructions when executed cause the processor to image select sub-regions of an analysis chamber, the instructions determine the select sub-regions of an analysis chamber for imaging based on a stored predetermined distribution representative of a presence or an absence of at least one constituent within a biologic fluid sample quiescently residing within the analysis chamber.

10. The apparatus of claim 9 , wherein the biologic fluid sample is blood and the constituents include at least one of white blood cells, red blood cells, plasma, or sphered red blood cells.

11. The apparatus of claim 10 , wherein the stored predetermined distribution is representative of the presence or absence of white blood cells within the select first sub-regions of the analysis chamber.

12. The apparatus of claim 10 , wherein the stored predetermined distribution is representative of the presence or absence of red blood cells within the select first sub-regions of the analysis chamber.

13. The apparatus of claim 9 , wherein the predetermined distribution representative of the at least one constituent within the biologic fluid sample quiescently residing within the analysis chamber is based on empirical data.

14. The apparatus of claim 9 , wherein the chamber is a microscope slide.

15. The apparatus of claim 9 , wherein the instructions when executed cause the processor to image the select first sub-regions of the analysis chamber using a first imaging method, and cause the processor to image select second sub-regions of the analysis chamber using a second imaging method, wherein the first imaging method is different than the second imaging method.

Assignees

• Abbott Point Of Care Inc

Inventors

• Xie Min
• Wardlaw Stephen C

Classifications

• G01N33/49 Primary

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title

• G01N21/6458

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• G01N15/0227

  using imaging; using holography · CPC title

• G01N33/483

  Physical analysis of biological material · CPC title

• G01N2015/0065

  Physics · mapped topic