Branched alpha glucans

Patent metadata

Field	Value
Publication number	US-10626428-B2
Application number	US-201615758443-A
Country	US
Kind code	B2
Filing date	Sep 12, 2016
Priority date	Sep 15, 2015
Publication date	Apr 21, 2020
Grant date	Apr 21, 2020

Abstract

The present invention relates to the field of poly- and oligosaccharides and their dietary effects. In particular it relates to a method of producing a branched α-glucan. Further aspects of the invention are a branched α-glucan comprising alternating α(1→4) and α(1→6) glucosidic linkages and having α(1→4,6) branching points, a food composition, and the use of an α-glucanotransferase enzyme for reducing the digestible carbohydrates of a starch containing food material.

First claim

The invention claimed is:

1. A method of producing an α-glucan with a ratio of branching of at least 8%, the method comprising;
contacting an α-glucanotransferase with a polysaccharide or oligosaccharide substrate having degree of polymerization (DP) of at least 3,
wherein the polysaccharide or oligosaccharide comprises at least two α(1→4) linked D-glucose residues at a non-reducing end and is selected from the group consisting of starch, starch derivatives, malto-oligosaccharides, amylose, amylopectin, maltodextrins, α(1→4) glucans and combinations thereof, and
wherein the α-glucanotransferase comprises an amino acid sequence having at least 95% identity to SEQ ID NO:1.

2. The method according to claim 1 , wherein the production of α-glucan is stopped before the reaction between the polysaccharide or oligosaccharide substrate and the α-glucanotransferase enzyme has reached completion.

3. The method according to claim 1 , wherein the polysaccharide or oligosaccharide substrate is contacted with the α-glucanotransferase enzyme at a temperature of between 30° C. and 75° C. and a pH of between 4.8 and 8.0.

4. The method according to claim 1 , wherein the α-glucan with a ratio of branching of at least 8% has less than 1.0% consecutive α(1→6) glucosidic linkages.

5. The method according to claim 1 , wherein the α-glucan with a ratio of branching of at least 8% has less than 0.5% consecutive α(1→6) glucosidic linkages.

6. The method according to claim 1 , wherein the α-glucan with a ratio of branching of at least 8% has no consecutive α(1→6) glucosidic linkages.

7. The method according to claim 1 , wherein the α-glucanotransferase comprises an amino acid sequence having at least 96% identity to SEQ ID NO:1.

8. The method according to claim 1 , wherein the α-glucanotransferase comprises an amino acid sequence having at least 97% identity to SEQ ID NO:1.

9. The method according to claim 1 , wherein the α-glucanotransferase comprises an amino acid sequence having at least 98% identity to SEQ ID NO:1.

10. The method according to claim 1 , wherein the α-glucanotransferase comprises an amino acid sequence having at least 99% identity to SEQ ID NO:1.

11. The method according to claim 1 , wherein the α-glucanotransferase has an amino acid sequence of SEQ ID NO:1.

Assignees

• Nestec Sa
• Nestle Sa

Inventors

• Gangoiti Munecas Joana
• Van Leeuwen Sander Sebastiaan
• Dijkhuizen Lubbert
• Vafeiadi Christina
• Lamothe Lisa

Classifications

• C12P19/18

  produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins · CPC title

• A23L29/225

  Farinaceous thickening agents other than isolated starch or derivatives · CPC title

• A61K31/715

  Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters · CPC title

• C08B37/0009

  alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof · CPC title

• A61K31/716

  Glucans · CPC title