Substrates and methods for collection, stabilization and elution of biomolecules

Patent metadata

Field	Value
Publication number	US-10625242-B2
Application number	US-201615285986-A
Country	US
Kind code	B2
Filing date	Oct 5, 2016
Priority date	Apr 30, 2012
Publication date	Apr 21, 2020
Grant date	Apr 21, 2020

Abstract

A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described.

First claim

What is claimed is:

1. A paper substrate for collection, stabilization and storage of a biological specimen or sample, the paper substrate consisting essentially of:
(a) a non-dissolvable material selected from the group consisting of cellulose, cellulose acetate and combinations thereof; and
(b) a trisaccharide impregnated in the non-dissolvable material.

2. The paper substrate of claim 1 , wherein the trisaccharide is melezitose, raffinose, maltotriulose, isomaltotriose, nigerotriose, maltotriose, kestose, or a combination thereof.

3. The paper substrate of claim 1 , wherein the trisaccharide is melezitose.

4. A method for extracting, stabilizing and eluting a biomolecule from a sample, the method comprising:
contacting the sample to the paper substrate of claim 1 ;
drying the sample to a water content of less than 2%; and
eluting the biomolecule from the sample dried on the paper substrate by rehydrating the paper substrate in an elution buffer,
wherein the biomolecule comprises a protein, a peptide, an amino acid, an enzyme, an antibody, or combinations thereof.

5. The method of claim 4 , wherein 70 to 90% of the biomolecule is recovered from the substrate.

6. The method of claim 4 , wherein the biomolecule is eluted in an intact form.

7. The method of claim 4 , wherein the material is cellulose.

8. The paper substrate of claim 1 , wherein the material consists of cellulose.

9. The paper substrate of claim 1 , wherein the material consists of cellulose acetate.

10. The paper substrate of claim 1 , wherein the material consists of cellulose and the trisaccharide is melezitose.

11. The paper substrate of claim 1 , wherein the material is cellulose, and the trisaccharide is melezitose, raffinose, maltotriulose, isomaltotriose, nigerotriose, maltotriose, kestose, or a combination thereof.

12. The method of claim 4 , further comprising storing the dried sample on the substrate for several weeks.

13. The method of claim 4 , further comprising washing the substrate before eluting the biomolecule.

14. The paper substrate of claim 1 , wherein the paper substrate consists of the non-dissolvable material and the trisaccharide.

Assignees

• Gen Electric

Inventors

• Kovacs Ernest William
• Mondello Frank John
• Kvam Erik Leeming
• Li Bing

Classifications

• B01J20/3242

  Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group · CPC title

• C07K14/545

  IL-1 · CPC title

• B01J20/22

  comprising organic material · CPC title

• B01J20/3246

  having a well defined chemical structure · CPC title

• C12N15/1006

  by means of a solid support carrier, e.g. particles, polymers · CPC title