Slip chip device and methods

Patent metadata

Field	Value
Publication number	US-9415392-B2
Application number	US-201013257811-A
Country	US
Kind code	B2
Filing date	Mar 23, 2010
Priority date	Mar 24, 2009
Publication date	Aug 16, 2016
Grant date	Aug 16, 2016

Abstract

A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position.

First claim

The invention claimed is:

1. A reaction system for carrying out a reaction, the reaction system comprising:
a first part having a first surface;
a plurality of first areas located along a portion of the first surface, each of the plurality of first areas configured to maintain at least one first substance;
a second part having a second surface opposed to the first surface; a plurality of second areas located along a portion of the second surface, each of the plurality of second areas configured to maintain at least one second substance; and
an inlet duct,

wherein at least one of the first surface of the first part and the second surface of the second part is configured to move relative to the other between a first position wherein the plurality of first areas are not exposed to any of the plurality of second areas, and a second position, wherein at least one of the plurality of first areas is only exposed to one of the plurality of the second areas, wherein the inlet duct is in fluidic communication via a continuous fluidic path within said first and second parts with each of said plurality of first areas only in said first position or only in said second position, and wherein the first part and the second part are engaged with each other before and after the relative motion.

2. The reaction system of claim 1 , wherein the reaction system is in the first position and at least one of the plurality of first areas contains a reagent.

3. The reaction system of claim 1 , wherein the reaction system is in the first position and at least one of the plurality of second areas contains a reagent.

4. The reaction system of claim 2 or 3 , wherein the reagent comprises at least one component selected from the group consisting of a nucleic acid, an enzyme, a polymerase enzyme, an antibody, a bacterium, a crystallization agent, a protein, a peptide, and a mammalian cell.

5. The reaction system of claim 2 or 3 , further comprising a fluid that is immiscible with the reagent.

6. The reaction system of claim 1 , wherein the at least one surface of the first part and the second surface of the second part is configured to move relative to the other from the first position to the second position.

7. The reaction system of claim 1 , wherein the at least one of the first surface of the first part and the second surface of the second part is configured to move relative to the other from the second position to the first position.

8. The reaction system of claim 1 , further comprising: a third part having a third surface,
a fourth surface on the second part,
wherein a portion of the plurality of second areas is located along the fourth surface, and the third surface is opposed to the fourth surface, and
at least one third area located along a portion of the third surf ace, the at least one third area configured to maintain at least one third substance, wherein at least one of the third surface of the third part and the fourth surface of the second part is configured to move relative to the other between a third position, where the at least one third area is not exposed to the plurality of second areas, and a fourth position, where one of

the at least one third areas is only exposed to one of the plurality of second areas, to form a closed system.

9. The reaction system of claim 1 , wherein the at least one of the first surface of the first part and the second surface of the second part is configured to move in a direction substantially perpendicular to the normal of the first surface of the first part when moving from the first position to the second position.

10. The reaction system of claim 1 , wherein when in the second position, one of the plurality of second areas is simultaneously exposed to at least two of the plurality of first areas.

11. The reaction system of claim 1 , wherein said plurality of first areas is arranged in a first pattern and wherein said plurality of second areas is arranged in a complementary pattern, wherein the movement from the first position to the second position allows at least one of the plurality of first areas to be exposed to a respective second area.

12. The reaction system of claim 11 , wherein either the number of first areas equals the number of second areas, the number of first areas is greater than the number of second areas or the number of first areas is less than the number of second areas.

13. The reaction system for carrying out a reaction of claim 1 , wherein at least two of the first and second areas differ in volume.

14. The reaction system device of claim 1 , further comprising a substrate disposed between the first surface and the second surface.

15. The reaction system of claim 1 , wherein at least one of the plurality of first and second areas comprises a well.

16. The reaction system of claim 1 , wherein at least one of the plurality of first and second areas comprises a surface pattern.

17. The reaction system of claim 1 , wherein at least two areas on the same surface differ in depth.

18. The reaction system of claim 1 , wherein the at least one of the first surface of the first part and the second surface of the second part is configured to slide relative to the other either from the first position to the second position, from the second position to the first position, or back and forth between the first and second positions.

19. The reaction system of claim 1 ,
further comprising an intermediate third part disposed between the first surface of the first part and the second surf ace of the second part, the intermediate third part having an opening formed therethrough;
wherein the first part, the second part and the intermediate third part are configured to slide relative to one another, from the first position where the plurality of first areas is not exposed to the plurality of second areas via the opening to the second position where at least one of the plurality of first areas is exposed to at least one of the plurality of second areas via the opening.

20. The reaction system of claim 1 , wherein at least one of said plurality of first areas and at least one of said plurality of second areas together form a closed system in said second position.

21. The reaction system of claim 1 , wherein said inlet duct is in a branch-like formation.

Assignees

• Ismagilov Rustem F
• Du Wenbin
• Li Liang
• Shen Feng
• Nichols Kevin Paul Flood
• Chen Delai
• Kreutz Jason Eugene
• Univ Chicago

Inventors

• Ismagilov Rustem F
• Du Wenbin
• Li Liang
• Shen Feng
• Nichols Kevin Paul Flood
• Chen Delai
• Kreutz Jason Eugene

Classifications

• B01L3/502738 Primary

  characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746) · CPC title

• B01L2300/0893

  having a very large number of wells, microfabricated wells · CPC title

• B01L2400/065

  sliding valves · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• B01L2300/0809

  rectangular shaped · CPC title