Mass spectrometry analysis of microorganisms in samples

Patent metadata

Field	Value
Publication number	US-10622198-B2
Application number	US-201816224191-A
Country	US
Kind code	B2
Filing date	Dec 18, 2018
Priority date	Jun 25, 2013
Publication date	Apr 14, 2020
Grant date	Apr 14, 2020

Abstract

The invention generally relates to systems and methods for mass spectrometry analysis of microorganisms in samples.

First claim

What is claimed is:

1. A system for analyzing an analyte in a biological sample, the system comprising:
a capture module, the module configured to capture an analyte from a human tissue or body fluid in an ambient environment and generate ions of the analyte, wherein the capture module comprises a cartridge and a porous substrate within the cartridge that is connected to a voltage source; and
a mass analyzer operably coupled to the capture module to receive the generated ions of the contaminant.

2. The system according to claim 1 , wherein the porous substrate is discrete from a flow of solvent.

3. The system according to claim 1 , wherein the porous substrate tapers to a distal tip.

4. The system according to claim 1 , wherein the capture module further comprises a gas-flow generating device that directs flow of the gas through the porous substrate.

5. The system according to claim 1 , further comprising a solvent applied to the porous substrate.

6. The system according to claim 5 , wherein the solvent comprises an internal standard.

7. The system according to claim 1 , wherein the mass analyzer is for a mass spectrometer or a handheld mass spectrometer.

8. The system according to claim 7 , wherein the mass analyzer is selected from the group consisting of: a quadrupole ion trap, a rectalinear ion trap, a cylindrical ion trap, a ion cyclotron resonance trap, an orbitrap, a time of flight, a Fourier Transform ion cyclotron resonance, and sectors.

9. The system according to claim 1 , wherein the porous substrate is filter paper.

10. The system according to claim 9 , wherein the filter paper tapers to a distal tip.

11. A method for analyzing an analyte in a biological sample, the system comprising:
providing a capture module, the module configured to capture an analyte in a biological sample in an ambient environment and generate ions of the analyte, wherein the capture module comprises a cartridge and a porous substrate within the cartridge that is connected to a voltage source;
capturing the analyte in a biological sample to the porous substrate of the capture module;
generating ions of the contaminant held by the porous substrate via the voltage source that is coupled to the porous substrate; and
analyzing the generated ions of the analyte in a mass analyzer that is operably coupled to the capture module.

12. The method according to claim 11 , wherein the porous substrate is discrete from a flow of solvent.

13. The method according to claim 11 , wherein the porous substrate tapers to a distal tip.

14. The method according to claim 11 , wherein the capture module further comprises a gas-flow generating device that directs flow of the gas through the porous substrate.

15. The method according to claim 11 , further comprising applying a solvent to the porous substrate.

16. The method according to claim 15 , wherein the solvent comprises an internal standard.

17. The method according to claim 11 , wherein the mass analyzer is for a mass spectrometer or a handheld mass spectrometer.

18. The method according to claim 17 , wherein the mass analyzer is selected from the group consisting of: a quadrupole ion trap, a rectalinear ion trap, a cylindrical ion trap, a ion cyclotron resonance trap, an orbitrap, a time of flight, a Fourier Transform ion cyclotron resonance, and sectors.

19. The system according to claim 11 , wherein the porous substrate is filter paper.

20. The system according to claim 19 , wherein the filter paper tapers to a distal tip.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Hamid Ahmed Mohamed
• Jarmusch Alan Keith
• Ouyang Zheng

Classifications

• H01J49/0445 Primary

  with means for introducing as a spray, a jet or an aerosol (electrospray ion sources H01J49/165) · CPC title

• C12Q1/04

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title

• H01J49/16

  using surface ionisation, e.g. field-, thermionic- or photo-emission · CPC title

• C12N13/00 Primary

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title