High Fidelity Restriction Endonucleases
US-2024352437-A1 · Oct 24, 2024 · US
Thioesterases and their use
US10619139B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10619139-B2 |
| Application number | US-201816050493-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 31, 2018 |
| Priority date | Sep 24, 2010 |
| Publication date | Apr 14, 2020 |
| Grant date | Apr 14, 2020 |
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- Title
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- Abstract
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- Assignees and inventors
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- First independent claim
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- CPC / IPC classifications
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- Citations and related patents
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Abstract
Official abstract text for this publication.
Disclosed are nucleotide sequences encoding thioesterase enzymes, methods for their production, their use in methods to form thioesters, and their use in methods of screening for other wild type bacteria capable of producing said thioesterase enzymes. Also disclosed are compositions comprising thioesters produced by the methods disclosed herein.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of flavouring cheese comprising adding to a cheese during its manufacture process at least one thioesterase enzyme comprising at least one of the amino acid sequences with at least 90% identity to SEQ ID NOS 2, 4, 6, or 8, and/or at least one isolated transfected host cell comprising at least one of the nucleotide sequences with at least 90% identity to SEQ ID NOS 1, 3, 5 or 7. 2. The method of claim 1 , wherein the at least one thioesterase enzyme and/or at least one host cell is added as part of a starter culture. 3. The method of claim 1 , wherein the at least one thioesterase enzyme comprises an amino acid sequence with at least 90% identity to SEQ ID NO 2. 4. The method of claim 1 , wherein the at least one thioesterase enzyme comprises an amino acid sequence with at least 90% identity to SEQ ID NO 4. 5. The method of claim 1 , wherein the at least one thioesterase enzyme comprises an amino acid sequence with at least 90% identity to SEQ ID NO 6. 6. The method of claim 1 , wherein the at least one thioesterase enzyme comprises an amino acid sequence with at least 90% identity to SEQ ID NO 8. 7. The method of claim 1 , wherein the at least one thioesterase enzyme comprises at least one host cell comprising a nucleotide sequence with at least 90% identity to SEQ ID NO 1. 8. The method of claim 1 , wherein the at least one thioesterase enzyme comprises at least one host cell comprising a nucleotide sequence with at least 90% identity to SEQ ID NO 3. 9. The method of claim 1 , wherein the at least one thioesterase enzyme comprises at least one host cell comprising a nucleotide sequence with at least 90% identity to SEQ ID NO 5. 10. The method of claim 1 , wherein the at least one thioesterase enzyme comprises at least one host cell comprising a nucleotide sequence with at least 90% identity to SEQ ID NO 7. 11. A method of obtaining a flavoured cheese product or a cheese flavoured product comprising adding to a cheese during its manufacture process at least one thioesterase enzyme comprising at least one of the amino acid sequences with at least 90% identity to SEQ ID NOS 2, 4, 6, or 8, and/or at least one isolated transfected host cell comprising at least one of the nucleotide sequences with at least 90% identity to SEQ ID NOS 1, 3, 5 or 7. 12. The method of claim 11 , wherein the at least one thioesterase enzyme comprises an amino acid sequence with at least 90% identity to SEQ ID NO 2. 13. The method of claim 11 , wherein the at least one thioesterase enzyme comprises an amino acid sequence with at least 90% identity to SEQ ID NO 4. 14. The method of claim 11 , wherein the at least one thioesterase enzyme comprises an amino acid sequence with at least 90% identity to SEQ ID NO 6. 15. The method of claim 11 , wherein the at least one thioesterase enzyme comprises an amino acid sequence with at least 90% identity to SEQ ID NO 8. 16. The method of claim 11 , wherein the at least one thioesterase enzyme comprises at least one host cell comprising a nucleotide sequence with at least 90% identity to SEQ ID NO 1. 17. The method of claim 11 , wherein the at least one thioesterase enzyme comprises at least one host cell comprising a nucleotide sequence with at least 90% identity to SEQ ID NO 3. 18. The method of claim 11 , wherein the at least one thioesterase enzyme comprises at least one host cell comprising a nucleotide sequence with at least 90% identity to SEQ ID NO 5. 19. The method of claim 11 , wherein the at least one thioesterase enzyme comprises at least one host cell comprising a nucleotide sequence with at least 90% identity to SEQ ID NO 7.
Assignees
Inventors
Classifications
- C12N9/16Primary
acting on ester bonds (3.1) · CPC title
Dairy flavours · CPC title
Thioester hydrolases (3.1.2) · CPC title
Enzymes other than milk clotting enzymes, e.g. lipase, beta-galactosidase · CPC title
Preparation of sulfur-containing organic compounds · CPC title
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Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US10619139B2 cover?
- Disclosed are nucleotide sequences encoding thioesterase enzymes, methods for their production, their use in methods to form thioesters, and their use in methods of screening for other wild type bacteria capable of producing said thioesterase enzymes. Also disclosed are compositions comprising thioesters produced by the methods disclosed herein.
- Who is the assignee on this patent?
- Givaudan Sa, Univ Utah State, Wisconsin Alumni Res Found
- What technology area does this patent fall under?
- Primary CPC classification C12N9/16. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 14 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).