Herbicide-resistant hydroxyphenylpyruvate dioxygenases

Patent metadata

Field	Value
Publication number	US-10619138-B2
Application number	US-201415114180-A
Country	US
Kind code	B2
Filing date	Aug 12, 2014
Priority date	Aug 12, 2013
Publication date	Apr 14, 2020
Grant date	Apr 14, 2020

Abstract

The present invention refers to method for producing a transgenic plant with increased herbicide tolerance or resistance as compared to a corresponding non-transformed wild type plant, comprising transforming a plant cell or a plant cell nucleus or a plant tissue with a nucleic acid molecule encoding a HPPD polypeptide, as well as to the nucleic acid, and plants with increased HPPD-inhibiting herbicide tolerance or resistance comprising the nucleic acid of the invention.

First claim

The invention claimed is:

1. A recombinant nucleic acid molecule having a nucleic acid sequence selected from the group consisting of:
(a) nucleic acid sequences encoding the HPPD polypeptide comprising the sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, or 32;
(b) nucleic acid sequences comprising the sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, or 31;
(c) nucleic acid sequences, which, as a result of the degeneracy of the genetic code, can be derived from a HPPD polypeptide sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, or 32;
(d) nucleic acid sequences having 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or more, identity with the nucleic acid sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, or 31; and
(e) nucleic acid sequences encoding a HPPD polypeptide having 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, or more, identity with the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, or 32;
wherein the nucleic acid sequence (i) is operatively attached to a heterologous plant regulatory sequence, and
(ii) encodes a polypeptide having HPPD activity observable by its production of homogentisic acid from 4-hydroxyphenylpyruvate, and having herbicide tolerance to an HPPD-inhibiting herbicide observable by the polypeptide's having a TI value of 0.1 or more toward said herbicide, according to the formula

T
⁢

⁢
I

=

k
cat

×

K
i

K
m

,

said TI value, in comparison to the TI value of the Arabidopsis thaliana HPPD having the amino acid sequence of SEQ ID NO:42, demonstrating that the encoded HPPD has increased tolerance, toward said herbicide, the HPPD-inhibiting herbicide being selected from:
[3-(4,5-dihydroisoxazol-3-yl)-2-methyl-4-methylsulfonyl-phenyl]-(5-hydroxy-1-methyl-pyrazol-4-yl)methanone (Inhibitor 1);
(2-(4-methylsulfonyl-2-nitro-benzoyl)cyclohexane-1,3-dione) (Inhibitor 2);
2-hydroxy-3-[2-(2-methoxyethoxymethyl)-6-(trifluoromethyl)pyridine-3-carbonyl]bicyclo [3.2.1]oct-2-en-4-one (Inhibitor 3);
N-12,6-dichloro-3-(8-hydroxy-5,5-dimethyl-6,6-dioxo-thiopyrano[4,3-b]pyridin-7-yl)phenyl]-N-methyl-methanesulfonamide (Inhibitor 4);
7-[2-ethylsulfonyl-4-(trifluoromethyl)phenyl]-5,5-dimethyl-6,6-dioxo-thiopyrano[4,3-b]pyridin-8-ol (Inhibitor 5);
N-[2,6-dichloro-3-(8-hydroxy-5,5-dimethyl-6,6-dioxo-thiopyrano [4,3-b]pyridin-7-yl)phenyl]-N-methyl-acetamide (Inhibitor 6);
7-[2,4-dichloro-3-(3-methyl-4,5-dihydroisoxazol-5-yl)phenyl]-5,5-dimethyl-6,6-dioxo-thiopyrano[4,3-b]pyridin-8-ol (Inhibitor 7);
7-(2,6-dichloro-3-pyridyl)-5,5-dimethyl-6,6-dioxo-thiopyrano[4,3-b]pyridin-8-ol (Inhibitor 8);
7-(2-bromo-3-chloro-6-fluoro-phenyl)-5,5-dimethyl-6,6-dioxo-thiopyrano[4,3-b]pyridin-8-ol (Inhibitor 9);
2-methyl-N-(4-methyl-1,2,5-oxadiazol-3-yl)-3,4-bis(methylsulfonyl)benzamide (Inhibitor 10); and
2,4-dichloro-6-fluoro-3-methylsulfanyl-N-(1-methyltetrazol-5-yl)benzamide (Inhibitor 11).

2. A nucleic acid construct comprising the nucleic acid molecule of claim 1 and one or more regulatory elements.

3. A vector comprising the nucleic acid molecule of claim 1 .

4. A plant cell nucleus, plant cell, plant tissue, propagation material, pollen, progeny, harvested material or a plant comprising the nucleic acid molecule of claim 1 .

5. A transgenic plant cell nucleus, cell, tissue, propagation material, seed, pollen, progeny, or part, resulting in a transgenic plant with increase herbicide tolerance or resistance after regeneration; or a transgenic plant with increased herbicide tolerance or resistance, or a part thereof, with said herbicide tolerance or resistance increased as compared to a corresponding wild type; wherein said transgenic plant cell nucleus, cell, tissue, propagation material, seed, pollen, progeny, part, or transgenic plant or part thereof, comprises the nucleic acid molecule of claim 1 .

6. The transgenic-plant cell nucleus, transgenic plant cell, transgenic plant or part thereof of claim 5 derived from a monocotyledonous plant.

7. The transgenic plant cell nucleus, transgenic plant cell, transgenic plant or part thereof of claim 5 derived from a dicotyledonous plant.

8. The transgenic plant cell nucleus, transgenic plant cell, transgenic plant or part thereof of claim 5 , wherein the corresponding plant is selected from the group consisting of maize, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, oil seed rape, including canola and winter oil seed rape, manihot, pepper, sunflower, sugar cane, sugar beet, flax, borage, safflower, linseed, primrose, rapeseed, turnip rape, tagetes, solanaceous plants comprising potato, tobacco, eggplant, tomato, Vicia species, pea, alfalfa, coffee, cacao, tea, Salix species, oil palm, coconut, perennial grass, forage crops and Arabidopsis thaliana.

9. The transgenic plant cell nucleus, transgenic plant cell, transgenic plant or part thereof of claim 5 , wherein the plant is selected from the group consisting of corn, soy, oil seed rape including canola and winter oil seed rape), cotton, wheat and rice.

10. A transgenic plant comprising one or more of plant cell nuclei or plant cells, progeny, seed or pollen or produced by a transgenic plant of claim 5 .

11. A method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of: a) providing, at said site, a plant that comprises at least one nucleic acid molecule of claim 1 , which is resistant or tolerant to a HPPD-inhibiting herbicide; and b) applying to said site an effective amount of said herbicide.

12. The method according to claim 11 , wherein the HPPD-inhibiting herbicide is an annelated heterocyclic compounds, or wherein the HPPD-inhibiting herbicide is selected from substituted pyridines, pyrazolone, isoxazole, triketone derivative herbicides, N-heterocyclyl-arylcarboxamides, bicycloarylcarboxamides, benzamides, heteroarylcarboxamides, and pyridylcarboxamides.

13. A method for growing the plant of claim 5 controlling weeds in the vicinity of said plant, said method comprising the steps of: a) growing said plant; and b) applying a herbicide composition comprising a HPPD-inhibiting herbicide to the plant and weeds, at a level of the herbicide that would inhibit the growth of a corresponding wild-type plant.

14. The recombinant nucleic acid molecule of claim 1 wherein the plant regulatory sequence is an algal, plant, or plant virus promoter.

Assignees

• Basf Se

Inventors

• Tresch Stefan
• Hutzler Johannes
• Pasternak Maciej
• Parra Rapado Liliana
• Lerchl Jens
• Besong Gilbert
• Kraus Helmut
• Witschel Matthias
• Paulik Jill Marie

Classifications

• C12Y113/11027

  4-Hydroxyphenylpyruvate dioxygenase (1.13.11.27) · CPC title

• A01N43/80

  five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2 · CPC title

• A01N43/08

  with oxygen as the ring hetero atom · CPC title

• C12N15/8274

  for herbicide resistance · CPC title

• A01N43/713

  having rings with four or more nitrogen atoms as the only ring hetero atoms · CPC title