Plant derived cell culture material

Patent metadata

Field	Value
Publication number	US-10612003-B2
Application number	US-201113881959-A
Country	US
Kind code	B2
Filing date	Oct 26, 2011
Priority date	Oct 27, 2010
Publication date	Apr 7, 2020
Grant date	Apr 7, 2020

Abstract

The present invention relates material that is useful in culturing and transferring cells as well as delivering cells. The material comprises plant derived cellulose nanofibers or derivatives thereof, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.

First claim

The invention claimed is:

1. A composition for cell culture or cell delivery, the composition comprising:
sterile, plant derived, mechanically disintegrated cellulose nanofibers and/or derivatives thereof, in a form of a three-dimensional hydrogel matrix having a nanofiber concentration ranging from about 0.01 to about 1.7 wt %, wherein the cellulose nanofibers and/or derivatives thereof are structurally type I cellulose; and
a plurality of cells homogeneously distributed within the three-dimensional matrix.

2. The composition according to claim 1 , wherein the diameter of cellulose nanofibers or nanofiber bundles in the cellulose nanofibers and/or derivatives thereof is less than 1 μm.

3. The composition according to claim 1 , wherein the derivatives of the cellulose nanofibers comprise chemically or physically modified derivatives of a cellulose nanofiber or nanofiber bundles.

4. The composition according to claim 1 , wherein the composition further comprises additives selected from the group consisting of extracellular matrix components, serum, growth factors and proteins.

5. The composition according to claim 1 , further comprising an immobilized cell or an immobilized enzyme.

6. The composition according to claim 1 , wherein the mechanically disintegrated cellulose nanofibers are acid-base pretreated.

7. The composition of claim 1 , wherein the mechanically disintegrated cellulose nanofibers form a gel at 0.5% concentration.

8. The composition of claim 7 , wherein the network structure of the cellulose nanofibers breaks down upon shearing.

9. The composition of claim 8 , wherein network structure of the cellulose nanofibers reforms after shearing ceases.

10. The composition according to claim 1 , wherein the at least one cell is a eukaryotic cell or a prokaryotic cell.

11. The composition according to claim 1 , wherein the at least one cell is a stem cell.

12. The composition according to claim 1 , wherein the cellulose nanofibers and/or derivatives thereof are inert and do not provide fluorescent background.

13. The composition according to claim 1 , wherein the cell culture or cell delivery composition is injectable.

14. The composition according to claim 1 , wherein the composition has a nanofiber concentration ranging from about 0.01 to about 1.0 wt %.

15. The composition according to claim 1 , wherein the composition has a solid-like viscosity at rest and a liquid-like viscosity upon shearing.

16. A method for producing a composition according to claim 1 , wherein said method comprises the steps of
providing microbially pure, plant derived, mechanically disintegrated cellulose nanofibers and/or derivatives thereof in a form of a two-dimensional or three-dimensional hydrogel matrix,
mixing together said cellulose nanofibers and/or derivatives thereof with water, and
providing living cells to the cellulose nanofibers,
wherein the cellulose nanofibers and/or derivatives thereof are structurally type I cellulose.

17. The method according to claim 16 , wherein the method further comprises combining the mixture with a medicament.

18. The method according to claim 16 , wherein the mechanically disintegrated cellulose nanofibers are acid-base pretreated.

Assignees

• Yliperttula Marjo
• Lauren Patrick
• Bhattacharya Madhushree
• Lou Yanru
• Laukkanen Antti
• Upm Kymmene Corp

Inventors

• Yliperttula Marjo
• Laurén Patrick
• Bhattacharya Madhushree
• Lou Yanru
• Laukkanen Antti

Classifications

• A61K47/38

  Cellulose; Derivatives thereof · CPC title

• C12N2533/78

  Cellulose · CPC title

• C08J2301/02

  Cellulose; Modified cellulose · CPC title

• C12N5/0606

  Pluripotent embryonic cells, e.g. embryonic stem cells [ES] (embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696) · CPC title

• C12N11/04

  entrapped within the carrier, e.g. gel or hollow fibres · CPC title