Methods of attaching a molecule-of-interest to a microtube
US-9464368-B2 · Oct 11, 2016 · US
US10597622B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10597622-B2 |
| Application number | US-201515531996-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 19, 2015 |
| Priority date | Oct 27, 2014 |
| Publication date | Mar 24, 2020 |
| Grant date | Mar 24, 2020 |
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An apparatus for hydrolysing a product, comprising a continuous conduit having an inlet for receiving the product and an outlet for discharging the product, the conduit being formed so as to follow a path that winds around a longitudinal axis as the conduit extends parallel to the longitudinal axis between the inlet and the outlet, and a heating system for heating at least part of the conduit. The conduit is rotatable about the longitudinal axis so as to transport product received in the inlet towards the outlet.
Opening claim text (preview).
The invention claimed is: 1. A method of hydrolyzing a product, the method comprising: (a) providing an apparatus, wherein the apparatus comprises: (a1) a continuous conduit having an inlet for receiving an initial product and an outlet for discharging a final product, wherein the conduit is formed so as to follow a path that winds around a longitudinal axis as the conduit extends parallel to the longitudinal axis between the inlet and the outlet, and wherein the conduit is rotatable about the longitudinal axis so as to transport initial product received in the inlet towards the outlet; and (a2) a heating system for heating at least part of the conduit; (b) introducing into the conduit via the inlet the initial product, wherein the initial product comprises a product-enzyme mixture that includes a product to be hydrolyzed and one or more enzymes; (c) heating at least part of the conduit using the heating system to hydrolyze the product-enzyme mixture and form the hydrolyzed product; and (d) rotating the conduit about the longitudinal axis so as to transport the hydrolyzed product mixture towards the outlet of the conduit. 2. The method of claim 1 , wherein the rotating step is carried out for between 30 and 60 minutes before the hydrolyzed product exits the outlet of the conduit. 3. The method of claim 1 , wherein the heating step is carried out at a temperature of between 40 and 70° C. 4. The method of claim 1 , wherein the rotating step comprises rotating the conduit in a forward direction and rotating the conduit in a backward direction, wherein over a predetermined period of time the conduit is rotated in the forward direction by a greater amount relative to the backward direction. 5. The method of claim 1 , wherein the product to be hydrolyzed comprises seafood. 6. The method of claim 1 , wherein the one or more enzymes comprises a protease, an alcalase, or a combination thereof. 7. The method of claim 1 , wherein the one or more enzymes form below 10% by mass of the initial product. 8. The method of claim 1 , wherein the heating system includes a tank surrounding at least part of the conduit, wherein the tank is configured for containing a heated fluid so as to heat the conduit. 9. The method of claim 8 , wherein the tank further includes a heater for heating fluid contained in the tank. 10. The method of claim 8 , wherein the tank is configured to receive a heated fluid. 11. The method of claim 8 , wherein the tank includes a bottom section and a top section, wherein the top section is moveable relative to the bottom section between a closed configuration in which the bottom and top sections form a closed chamber, and an open configuration in which the bottom and top sections do not form a closed chamber. 12. The method of claim 1 , wherein the apparatus further comprises a driver for rotating the conduit so as to transport product received in the inlet towards the outlet. 13. The method of claim 12 , wherein the driver comprises a motor rotatably coupled to the conduit. 14. The method of claim 1 , wherein the conduit is helical over at least a portion of its length. 15. The method of claim 1 , further comprising a pump for introducing the product into the inlet.
Tubular (C12M23/08, C12M23/16 take precedence) · CPC title
Mobile; Means for transporting the apparatus (transportable laboratories B01L1/52) · CPC title
Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes · CPC title
with horizontal or inclined stirrer shaft or axis · CPC title
Addition of, or treatment with, microorganisms or enzymes · CPC title
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