Systems and methods including a rotary valve for at least one of sample preparation or sample analysis

Patent metadata

Field	Value
Publication number	US-10596569-B2
Application number	US-201515315638-A
Country	US
Kind code	B2
Filing date	Jun 3, 2015
Priority date	Jun 5, 2014
Publication date	Mar 24, 2020
Grant date	Mar 24, 2020

Abstract

Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

First claim

What is claimed is:

1. A system comprising:
a fluidic network comprising a sample channel, a reaction chamber, and a reservoir, the sample channel being in flow communication with a sample port that is configured to receive a biological sample, the reaction chamber having at least one optically transparent surface and a detector surface, the detector surface spaced apart from the at least one optically transparent surface, the detector surface having a plurality of reaction sites;
a pump assembly in flow communication with the fluidic network;
at least one light sensor configured to detect light signals generated by a reaction of at least one of the plurality of reaction sites within the reaction chamber;
a rotary valve comprising a flow channel, a bridge channel, and a feed port, the feed port fluidically coupled to the reaction chamber, the rotary valve rotatable between a first valve position, a second valve position, and a third valve position, the flow channel fluidically coupling the feed port of the rotary valve to the sample channel when the rotary valve is in the first valve position, the flow channel fluidically coupling the feed port to the reservoir when the rotary valve is in the second valve position, and the bridge channel fluidically coupling the reservoir with a first port when the rotary valve is in the third valve position, wherein the pump assembly is configured to induce a flow of the biological sample toward the reaction chamber when the rotary valve is in the first valve position, wherein the pump assembly is further configured to induce a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position, and wherein the pump assembly is further configured to induce a flow of the reaction component from the reservoir toward the first port when the rotary valve is in the third position, wherein the rotary valve rotates about an axis, the feed port is aligned with the axis and fluidically couples the flow channel and the reaction chamber.

2. The system of claim 1 , wherein the pump assembly comprises a single pump that is in flow communication with the reaction chamber and is located downstream with respect to the reaction chamber.

3. The system of claim 1 , wherein the rotary valve retains the biological sample in the flow channel as the rotary valve rotates from the first valve position to a fourth valve position, the pump assembly inducing a flow of the biological sample into a buffer reservoir when the rotary valve is in the-fourth valve position.

4. The system of claim 3 , wherein the sample channel is a first sample channel and the biological sample is a first biological sample, the fluidic network including a second sample channel having a second biological sample, the rotary valve rotatable to a fifth valve position such that the flow channel is in flow communication with the second sample channel, the pump assembly further configured to induce a flow of the second biological sample in the second sample channel into the flow channel, wherein the rotary valve retains the second biological sample in the flow channel as the rotary valve rotates from the fifth valve position to the fourth valve position, the pump assembly inducing a flow of the second biological sample therein into the reservoir when the rotary valve is in the fourth valve position.

5. The system of claim 1 , wherein the reservoir is a first reservoir, the fluidic network further comprising a second reservoir, wherein the rotary valve moves to a fourth valve position such that the flow channel fluidically couples the second reservoir and the reaction chamber.

6. The system of claim 1 , wherein the sample channel is a first sample channel and the fluidic network comprises a second sample channel.

7. The system of claim 6 , wherein each of the first and second sample channels is in flow communication with the rotary valve through a common supply port.

8. The system of claim 6 , further comprising a channel valve coupled to the sample channel, the channel valve movable between first and second positions to block flow and to permit flow, respectively, through the sample channel.

9. The system of claim 1 , wherein the at least one light sensor is positioned adjacent to the detector surface of the reaction chamber and comprises an imaging detector configured to detect light signals from the reactions within the reaction chamber.

10. The system of claim 1 , further comprising a system controller that automatically controls the rotary valve and the pump assembly to conduct iterative cycles of a sequencing-by-synthesis (SBS) protocol.

11. The system of claim 1 , wherein the reaction is one of an associative binding event, dissociative binding event, release of a fluorescently labeled biomolecule from an analyte-of-interest, chemical transformation, chemical change, chemical interaction, fluorescence, luminescence, bioluminescence, chemiluminescence, biological reactions, nucleic acid replication, nucleic acid amplification, nucleic acid hybridization, nucleic acid ligation, phosphorylation, enzymatic catalysis, receptor binding, ligand binding, change in ion concentration, chemical binding interaction, or the addition or elimination of a proton.

Assignees

• Ilumina Inc
• Illumina Inc

Inventors

• Bohm Sebastian
• Aravanis Alex
• Hsiao Alexander
• Javanmardi Behnam
• Khurana Tarun
• Tran Hai Quang
• Aghababazadeh Majid
• Bowen M Shane
• Boyanov Boyan
• Buermann Dale

Classifications

• B01L3/502738 Primary

  characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746) · CPC title

• B01L2200/10

  Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept · CPC title

• B01L3/50273

  characterised by the means or forces applied to move the fluids · CPC title

• F16K2099/0084

  Chemistry or biology, e.g. "lab-on-a-chip" technology · CPC title

• F16K99/0028

  Valves having multiple inlets or outlets · CPC title