Transgenic plants with enhanced agronomic traits

Patent metadata

Field	Value
Publication number	US-10570408-B2
Application number	US-201715732622-A
Country	US
Kind code	B2
Filing date	Dec 5, 2017
Priority date	Jul 10, 2007
Publication date	Feb 25, 2020
Grant date	Feb 25, 2020

Abstract

This invention provides recombinant DNA constructs, transgenic plant nuclei and cells with such recombinant DNA construct for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

First claim

What is claimed is:

1. A method for manufacturing transgenic seeds that can be used to produce a crop of transgenic plants with an enhanced trait resulting from expression of a DNA segment in a plant cell nucleus comprising a stably integrated recombinant DNA construct comprising a promoter that is functional in a plant cell and that is operably linked to a DNA segment encoding a protein comprising an amino acid sequence having at least 90% amino acid sequence identity to SEQ ID NO: 373, or a homolog of SEQ ID NO:373 thereof, wherein said method comprises:
(a) screening a population of plants for said enhanced trait and said recombinant DNA construct, wherein said enhanced trait is increased yield;
(b) selecting from said population one or more plants that exhibit said trait at a level greater than the level that said trait is exhibited in control plants; and
(c) collecting seeds from the selected plant.

2. The method of claim 1 , wherein said method further comprises:
(a) verifying that said recombinant DNA construct is stably integrated in said selected plants; and
(b) analyzing tissue of said selected plant to determine the expression of the protein.

3. The method of claim 2 wherein said seed is corn, soybean, cotton, alfalfa, canola wheat or rice seed.

4. The method of claim 1 wherein the selected plant is a herbicide tolerant corn plant.

5. The method of claim 2 wherein said transgenic plant cell is homozygous for said recombinant DNA construct.

6. The method of claim 2 wherein the transgenic plant cell further comprises DNA expressing a protein that provides tolerance from exposure to an herbicide applied at levels that are lethal to a wild type of said plant cell.

7. The method of claim 4 wherein said herbicide is a glyphosate, dicamba, or glufosinate compound.

8. The method of claim 1 wherein the protein has SEQ ID NO: 373.

9. The method of claim 3 wherein the yield is determined by measuring the number of kernels.

10. The method of claim 1 wherein the homolog has SEQ ID NO:29625.

Assignees

• Monsanto Technology Llc

Inventors

• Deng Molian
• Xie Zhidong

Classifications

• C12N15/8273 Primary

  for drought, cold, salt resistance · CPC title

• C12N15/8261 Primary

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title