Transgenic plants with enhanced agronomic traits

Patent metadata

Field	Value
Publication number	US-2016230183-A1
Application number	US-201614998728-A
Country	US
Kind code	A1
Filing date	Feb 8, 2016
Priority date	Jul 10, 2007
Publication date	Aug 11, 2016
Grant date	—

Abstract

This invention provides recombinant DNA constructs, transgenic plant nuclei and cells with such recombinant DNA construct for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

First claim

What is claimed is:

1 . A recombinant DNA construct comprising a promoter that is functional in a plant cell and that is operably linked to a DNA segment that encodes:
a. at least one protein having an amino acid sequence comprising a Pfam domain module selected from the group consisting of zf-CCCH, PALP, GAF::HisKA::HATPase_c, TPR_2::TPR_1::TPR_2::TPR_1::TPR_4::TPR_2::TPR_1, efhand::efhand, Spermine_synth, ELFV_dehydrog_N::ELFV_dehydrog, PFK, PAS 3::PAS_3::Pkinase, S1, GDC-P, SWIM, B12-binding::Radical_SAM, S1, LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1, Chloroa_b-bind, LRRNT_2::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::Pkinase, WD40::WD40, YABBY, Ldh_1_N::Ldh_1_C, Sina, Na_H_antiport_1, ParBc, YABBY, Histone, Fe_bilin_red, Tryp alpha_amyl, Pyr_redox_2::Thioredoxin, E2F_TDP, CN_hydrolase, YDG_SRA::Pre-SET::SET, APC8::TPR 1::TPR 1::TPR_1, Ras, tRNA_anti::tRNA-synt_2, Auxin_inducible, PGI, S1, Chloroa_b-bind, Bac_globin, Glyco_hydro_17, MGS, Spermine_synth, LRRNT_2::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::Pkinase, Aa_trans, LRRNT_2::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::Pkinase_Tyr, Gln-synt_N::Gln-synt_C, SAP18, TPP_enzyme_N::TPP_enzyme_M::TPP_enzyme_C, zf-C3HC4, eIF-1a, RPE65, PBP, Pkinase, AA_permease, F-box::LRR_1::LRR_2, zf-CCCH::zf-CCCH, Lactamase_B::Flavodoxin_1::Flavin_Reduct, Bac_globin, DSPc, adh_short, Tim17, Oxidored_molyb::Mo.co_dimer::Cyt-b5::FAD_binding_6::NAD_binding_1, ubiquitin::ubiquitin::ubiquitin::ubiquitin, MBD, CXC::CXC, HSF_DNA-bind, Spermine_synth, AP2, Peptidase_S10, PALP, EIN3, Gln-synt_N::Gln-synt_C, 2OG-FelI_Oxy, Glyco_hydro_9, GDC-P, B3, PTPA, Acyltransferase, Isochorismatase, FMO-like, Molybdop_Fe4S4::Molybdopterin::Molydop_binding::Fer2_BFD, Lir1, Prismane, Fer2, DEAD::Helicase_C, Molybdop_Fe4S4::Molybdopterin::Molydop binding::Fer2_BFD, KNOX1::KNOX2::ELK, Glyoxalase, Sad1_UNC, Bac_globin, VPS28, PP2C, Pkinase::efhand::efhand::efhand, LEA_3, Peptidase_S10, Pkinase, CBFD_NFYB_HMF, Gln-synt_N::Gln-synt_C, Pyr_redox_2::Fer2_BFD::NIR_SIR_ferr::NIR_SIR, NUDIX::NUDIX, FAD_binding_3, GST_N::GST_C, SAM_decarbox, Acyltransferase, NTP_transferase, G-patch, 2OG-FelI_Oxy, Gln-synt_N::Gln-synt C, AAA::Vps4_C, Histone, Pkinase, TPR_1, F-box::Kelch_1::Kelch_1, Spermine_synth, Bac_globin, Bac_globin, zf-UBR, Homeobox::HALZ, Whirly, NAD_binding_1, PTR2, EIN3, 4HBT, adh_short, 2OG-FelI_Oxy, P-II, Myb_DNA-binding::Myb_DNA-binding, DAGK_cat, AP2, MFS_1, Chloroa_b-bind, DUF716, zf-Dof, CCT, Homeobox::HALZ, Histone, 2OG-FelI_Oxy, Globin, Pyr_redox_2::Fer2_BFD::NIR_SIR_ferr::NIR_SIR, Whirly, PsbP, bZIP_1, LRRNT_2::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::Pkinase_Tyr, Phi_1, BURP, Sterol_desat, DSPc, SNF5, Acyltransferase, GATase_2::Asn_synthase, adh_short, Homeobox::START, Pkinase, ParBc, SOUL, DNA_photolyase::FAD_binding_7, Pkinase_Tyr, NAD_Gly3P_dh_N::NAD_Gly3P_dh_C, Na_H_Exchanger, peroxidase, Oxidored_molyb::Mo-co_dimer::Cyt-b5::FAD_binding_6::NAD_binding_1, Hexokinase_1::Hexokinase_2, DUF716, S10_plectin, Thi4, p450, CCT, adh_short, PSI_PSAK, DUF640, Thioredoxin, Globin, Ank::Pkinase, DAGAT, RPE65, Ank::Pkinase, GSHPx, Gln-synt_N::Gln-synt_C, MtN3 slv::MtN3 slv, Allene ox cyc, IGPD, MBD, CorA, Response_reg, Histone, AAA, Ribosomal_L10e, Pkinase, DUF26::DUF26::Pkinase, p450, mTERF, AA_kinase, PBP, GUN4, Lactamase_B::Flavodoxin_1::Rubredoxin, C2, RRM_1::RRM_1, Histone, Alpha-amylase, HLH, Thioredoxin, Histone_HNS, Myb_DNA binding::Myb_DNA-binding, Cytochrom_C552, AP2::AP2, MtN3_slv::MtN3_slv, SHMT, ParBc, Mit_rib_S27, Ribosomal_S2, KNOX1::KNOX2::ELK, MFS_1, Glyco_transf_5::Glycos_transf_1, Cellulase, Ribosomal_L10e, Spermine_synth, Glyco_hydro_2_N::Glyco_hydro_2::Glyco_hydro_2_C, TP_methylase, AP2::AP2, Histone, Response_reg::CCT, Histone HNS, DUF1716, p450, GATA, Pkinase, Sugar_tr, Aa_trans, Pribosyltran, Ribosomal_L10e, HLH, PMSR, DnaJ::DnaJ_CXXCXGXG::DnaJ_C, DUF1005, Glyco_transf_5::Glycos_transf_1, Spermine_synth, S1::EIF_2_alpha, RGS, Na_sulph_symp, S1, MtN3_slv::MtN3_slv, Lactamase_B::Flavin_Reduct, LRRNT_2::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::LRR_1::Pkinase, Chloroa_b-bind, PTR2, Agglutinin, PLATZ, NPH3, Auxin_inducible, PTR2, GAT::HisKA::Response_reg, PsbQ, GSH_synth_ATP, GATase_2::Asn_synthase, PHP, FtsJ, DUF6::TPT, Proteasome, PsbW_2, Glyco_hydro_9, NAD_binding_2::6PGD, S1::EIF_2_alpha, Homeobox::START::MEKHLA, S1, Isoamylase_N::Alpha-amylase, E2F_TDP, and Rieske::PaO; b. a protein comprising an amino acid sequence with at least 90% identity to a consensus amino acid sequence selected from the group consisting of SEQ ID NO: 30526 through 30550; c. a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs 352, 493, 533 and 544, and homologs thereof listed in Table 8; d. a protein having an amino acid sequence having at least 70% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs 320 and 383; or e. a protein having an amino acid sequence selected from the group consisting of SEQ ID NO: 299 through SEQ ID NO: 30468; and wherein said recombinant DNA construct is stably integrated into a chromosome in a plant cell nucleus which is selected by screening a population of transgenic plants that have said recombinant DNA construct and an enhanced trait as compared to control plants that do not have said recombinant DNA construct in their nuclei; and wherein said enhanced trait is selected from group of enhanced traits consisting of enhanced water use efficiency, enhanced cold tolerance, enhanced heat tolerance, enhanced high salinity tolerance, enhanced shade tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil.

2 . A transgenic plant cell nucleus comprising a recombinant DNA construct of claim 1 .

3 . A transgenic plant cell having a plant cell nucleus of claim 2 .

4 . The transgenic plant cell of claim 3 wherein said transgenic plant cell is homozygous for said recombinant DNA construct.

5 . The transgenic plant cell of claim 3 further comprising DNA expressing a protein that provides tolerance from exposure to an herbicide applied at levels that are lethal to a wild type of said plant cell.

6 . The transgenic plant cell of claim 4 wherein said herbicide is a glyphosate, dicamba, or glufosinate compound.

7 . A transgenic plant comprising a plurality of plant cells of claim 3 .

8 . The transgenic plant of claim 7 wherein said transgenic plant is homozygous for said recombinant DNA construct.

9 . A transgenic seed comprising a recombinant DNA construct of claim 1 .

10 . The transgenic seed of claim 9 from a corn, soybean, cotton, canola, alfalfa, wheat or rice plant.

11 . A transgenic pollen grain comprising a recombinant DNA construct of claim 1 .

12 . A method for manufacturing transgenic seeds that can be used to produce a crop of transgenic plants with an enhanced trait resulting from expression of a DNA segment in a plant cell nucleus comprising a recombinant DNA construct of claim 1 , wherein said method comprises:
(a) screening a population of plants for said enhanced trait and said recombinant DNA construct, wherein individual plants in said population can exhibit said trait at a level less than, essentially the same as or greater than the level that said trait is exhibited in control plants which do not contain said recombinant DNA construct, wherein said enhanced trait is selected from the group of e

Assignees

• Monsanto Technology Llc

Inventors

• Abad Mark Scott
• Ahrens Jeffery E
• Augustine Alice Clara
• Bell Erin
• Bensen Robert J
• Venkatachalayya Srikanth
• Castiglioni Paolo
• Cerny R Eric
• Chittoor-Vijayanath Jaishree M
• Daley Maureen
• Deikman Jill
• Deng Molian
• Dezwaan Todd
• Duff Stephen
• Edgerton Michael D
• Fabbri Bradon J
• Fenner Jason
• Gabbert Karen
• Donnarummo Meghan Galligan
• Lee Garrett J
• Goldman Barry S
• Hawkins Deborah J
• He Steve
• Chen Xianfeng
• Karunanandaa Balasulojini
• Loida Paul
• Madappa Savitha
• Meister Robert J
• Peng Ming
• Nelson Donald E
• Pandravada Anand
• Patty Obed
• Petracek Marie
• Somaiah Rajani Monnanda
• Ravanello Monica P
• Johnson Richard
• Ruff Thomas G
• Sanders Rick A
• Savidge Beth
• Schwartz Steven H
• Char Shobha
• Sudarshana Padmini
• Sun Jindong
• Thompson-Mize Rebecca L
• Ursin Virginia
• Val Dale V
• Venkatesh Tymagondlu V
• Vidya Kammaradi
• Vijayalakshmi K
• Weber Todd C
• Wu Jingrui
• Zheng Wei
• Xie Zhidong
• Ramachandra Dhanalakshmi

Classifications

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title

• C12N15/8241

  Phenotypically and genetically modified plants via recombinant DNA technology · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12N15/8273 Primary

  for drought, cold, salt resistance · CPC title

• C12N15/8251

  Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis · CPC title