D-psicose 3-epimerase mutant with improved thermal stability, and continuous production of D-psicose using same
US-9217166-B2 · Dec 22, 2015 · US
Corynebacterium glutamicum for use in producing 2′-fucosyllactose
US10570399B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10570399-B2 |
| Application number | US-201715574028-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 24, 2017 |
| Priority date | Apr 25, 2016 |
| Publication date | Feb 25, 2020 |
| Grant date | Feb 25, 2020 |
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Abstract
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Disclosed are a recombinant Corynebacterium glutamicum ( C. glutamicum ) for producing fucosyllactose which is transformed to express α-1,2-fucosyltransferase, GDP-D-mannose-4,6-dehydratase (Gmd), GDP-L-fucose synthase (WcaG) and lactose permease (LacY), wherein the Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase, and a method for producing fucosyllactose using the same. According to the recombinant Corynebacterium glutamicum and the method for producing fucosyllactose according to the present invention, with use of a GRAS Corynebacterium glutamicum strain, which is safer than conventional Escherichia coli , 2′-fucosyllactose can be produced at a high concentration while overcoming drawbacks of conventional methods associated with industrial inapplicability resulting from low production concentrations.
First claim
Opening claim text (preview).
The invention claimed is: 1. A recombinant Corynebacterium glutamicum transformed to express α-1,2-fucosyltransferase, transformed to express GDP-D-mannose-4,6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase, and wherein the α-1,2-fucosyltransferase is encoded by fucT2 genes consisting of the nucleic acid sequence of SEQ ID NO: 6.
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Classifications
Phosphomannomutase (5.4.2.8) · CPC title
GDP-mannose 4,6-dehydratase (4.2.1.47), i.e. GMD · CPC title
Mannose-1-phosphate guanylyltransferase (2.7.7.13) · CPC title
Phosphotransferases with an alcohol group as acceptor (2.7.1) · CPC title
Galactoside 2-alpha-L-fucosyltransferase (2.4.1.69) · CPC title
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- What does patent US10570399B2 cover?
- Disclosed are a recombinant Corynebacterium glutamicum ( C. glutamicum ) for producing fucosyllactose which is transformed to express α-1,2-fucosyltransferase, GDP-D-mannose-4,6-dehydratase (Gmd), GDP-L-fucose synthase (WcaG) and lactose permease (LacY), wherein the Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase, and a method for producing …
- Who is the assignee on this patent?
- Seoul Nat Univ R&Db Foundation, Ap Tech Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N15/77. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 25 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).