Compositions and methods for enhanced production of enduracidin in a genetically engineered strain of streptomyces fungicidicus
US-2019359659-A1 · Nov 28, 2019 · US
Method for producing recombinant 11-de-O-methyltomaymycin
US10550397B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10550397-B2 |
| Application number | US-201314401109-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 13, 2013 |
| Priority date | May 14, 2012 |
| Publication date | Feb 4, 2020 |
| Grant date | Feb 4, 2020 |
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- Title
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Abstract
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The present invention provides a tomaymycin biosynthetic gene cluster of Streptomyces species FH6421, and its use for producing 11-de-O-methyltomaymycin.
First claim
Opening claim text (preview).
The invention claimed is: 1. An expression vector comprising the gene cluster of SEQ ID NO: 2 or a variant or fragment thereof wherein the variant or fragment encodes proteins with at least 90% amino acid sequence identity to SEQ ID NOs: 4 to 22. 2. A cell comprising the expression vector according to claim 1 . 3. A method for producing a cell that harbors the expression vector of claim 1 , the method comprising transforming a cell with the expression vector. 4. The method according to claim 3 , wherein the cell harbors the tomaymycin biosynthetic gene cluster of SEQ ID NO: 2. 5. The method according to claim 4 , wherein the cell is a Streptomyces strain. 6. The method according to claim 5 , wherein the Streptomyces strain is selected from the group consisting of Streptomyces achromogenes var. tomaymyceticus, Streptomyces species FH6421, and Streptomyces albus /pStW102tc. 7. The method according to claim 3 , wherein the cell is capable of producing 11-de-O-methyltomaymycin. 8. The expression vector according to claim 1 , comprising the nucleic acid sequence of SEQ ID NO: 3. 9. The cell according to claim 2 , wherein the cell is a Streptomyces cell. 10. The cell according to claim 2 , wherein the cell is a Streptomyces albus cell.
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Classifications
from Actinomyces; from Streptomyces (G) · CPC title
Heterocyclic compounds containing more than one system of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring system not provided for in groups C07D453/00 or C07D455/00 · CPC title
- C12N15/76Primary
for Actinomyces; for Streptomyces · CPC title
Genes encoding for enzymes or proenzymes · CPC title
Ortho-condensed systems · CPC title
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- What does patent US10550397B2 cover?
- The present invention provides a tomaymycin biosynthetic gene cluster of Streptomyces species FH6421, and its use for producing 11-de-O-methyltomaymycin.
- Who is the assignee on this patent?
- Sanofi Sa
- What technology area does this patent fall under?
- Primary CPC classification C12N15/76. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 04 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).