Cellular response to surface with nanoscale heterogeneous rigidity

Patent metadata

Field	Value
Publication number	US-10550365-B2
Application number	US-201715483754-A
Country	US
Kind code	B2
Filing date	Apr 10, 2017
Priority date	Apr 2, 2008
Publication date	Feb 4, 2020
Grant date	Feb 4, 2020

Abstract

An elastomeric substrate comprises a surface with regions of heterogeneous rigidity, wherein the regions are formed by exposing the elastomeric substrate to an energy source to form the regions such that the regions include a rigidity pattern comprising spots.

First claim

What is claimed is:

1. A method for fabricating a substrate, the method comprising:
forming a substrate of an elastomer, the substrate having a substrate surface; and
exposing one or more selected regions of the substrate surface to an energy source, the energy source configured to modify a rigidity of the one or more selected regions,
wherein the one or more selected regions form a specified pattern on the substrate surface, and wherein exposing the one or more selected regions to the energy source to modify the rigidity of the one or more selected regions forms a rigidity pattern on the substrate surface corresponding to the specified pattern, and
wherein the specified pattern is selected so that the rigidity pattern provides for a differential functional response from cells cultured upon the rigidity pattern.

2. The method according to claim 1 , wherein the energy source comprises at least one of a focused electron beam or deep ultraviolet light.

3. The method according to claim 1 , wherein the energy source initiates cross-linking of the elastomer in order to increase rigidity of the elastomer in the one or more selected regions.

4. The method according to claim 1 , wherein the elastomer comprises poly(dimethylsiloxane) or a poly(dimethylsiloxane)-based polymer.

5. The method according to claim 1 , wherein each of the one or more selected regions include at least one of microscale features or nanoscale features.

6. The method according to claim 1 , wherein forming the substrate comprises forming the substrate surface to have three-dimensional structures within the one or more selected regions, and wherein exposing the one or more selected regions to the energy source comprises locally modifying a rigidity of the three-dimensional structures.

7. The method according to claim 1 , wherein the differential functional response comprises at least one of: differential focal adhesion of the cells to the substrate surface; differential cell differentiation of the cells; differential immune response; or differential growth of the cells.

8. The method according to claim 1 , wherein the cells comprise at least one of stem cells, T-cells, cancer cells, nerve cells, osteoblasts, and muscle cells.

9. The method according to claim 1 , wherein the rigidity pattern comprises a repeating feature.

10. The method according to claim 9 , wherein the repeating feature includes a lateral dimension that is greater than or equal to 250 nanometers.

11. The method according to claim 9 , wherein the repeating feature includes a lateral dimension that is less than or equal to 250 nanometers.

12. The method according to claim 9 , wherein the repeating feature includes spots.

Assignees

• Univ Columbia

Inventors

• Biggs Manus J P
• Cooper Ryan
• Liao Jinyu
• Fazio Teresa Anne
• Dahlberg Carl Fredrik Oskar
• Kysar Jeffrey William
• Wind Samuel Jonas

Classifications

• C12N2535/10

  Patterned coating · CPC title

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• C12N2537/10

  Cross-linking · CPC title

• C12N2535/00

  Supports or coatings for cell culture characterised by topography · CPC title

• C12N5/0663

  Bone marrow mesenchymal stem cells (BM-MSC) · CPC title