Yeast, yeast extract containing gamma-Glu-Abu, and a method for producing the same

Patent metadata

Field	Value
Publication number	US-10537128-B2
Application number	US-201615352928-A
Country	US
Kind code	B2
Filing date	Nov 16, 2016
Priority date	Oct 5, 2010
Publication date	Jan 21, 2020
Grant date	Jan 21, 2020

Abstract

A yeast extract containing 0.2% or more of γ-Glu-Abu based on dry weight of the yeast extract is produced by culturing a yeast, such as Saccharomyces cervisiae or Candida utilis, in a medium containing a compound selected from Abu (L-2-aminobutyric acid) and γ-Glu-Abu (L-γ-glutamyl-L-2-aminobutyric acid), and preparing a yeast extract from the obtained cells.

First claim

The invention claimed is:

1. A method for producing a yeast extract comprising γ-Glu-Abu, comprising:
culturing yeast cells in a medium comprising at least one of Abu and γ-Glu-Abu to produce cultured cells; and
preparing a yeast extract from the cultured cells,
wherein the medium comprises 10 ppm or more of Abu, 1 ppm or more of γ-Glu-Abu, or both and wherein the yeast extract comprises 0.2% or more of γ-Glu-Abu based on a dry weight of the yeast extract.

2. The method according to claim 1 , wherein the yeast cells belong to the genus Saccharomyces or Candida.

3. The method according to claim 1 , wherein the yeast cells belong to Saccharomyces cervisiae or Candida utilis.

4. The method according to claim 1 , wherein the yeast cells have an increased activity of a γ-glutamylcysteine synthetase, an attenuated activity of a glutathione synthetase, or both.

5. The method according to claim 1 , wherein, in the culturing, the at least one of Abu and γ-Glu-Abu is continuously added to the medium.

6. The method according to claim 1 , further comprising:
preculturing the yeast cells in a medium which is free of Abu and γ-Glu-Abu prior to the culturing.

7. The method according to claim 1 , wherein the culturing comprises culturing the yeast cells such that the cultured cells contain γ-Glu-Abu in an amount of 0.04% or more, based on a dry weight of the cultured cells.

8. The method according to claim 1 , wherein the culturing comprises culturing the yeast cells such that the cultured cells contain γ-Glu-Abu in an amount of 0.4% or more, based on a dry weight of the cultured cells.

9. The method according to claim 4 , wherein the yeast cells further have an attenuated activity of a peptidase capable of decomposing an intracellular peptide.

10. The method according to claim 1 , wherein the method consists essentially of the culturing and the preparing.

11. The method according to claim 7 , wherein the method consists essentially of the culturing and the preparing.

12. The method according to claim 8 , wherein the method consists essentially of the culturing and the preparing.

13. The method according to claim 1 , wherein the medium comprises 50 ppm or more of Abu, 10 ppm or more of γ-Glu-Abu, or both.

14. The method according to claim 1 , wherein the yeast extract comprises 2.0% or more of γ-Glu-Abu based on a dry weight of the yeast extract.

15. The method according to claim 1 , further comprising, prior to the preparing of the yeast extract:
separating the cultured cells from the medium.

Assignees

• Ajinomoto Kk

Inventors

• Nishiuchi Hiroaki
• Morita Misato
• Hoshino Wataru
• Yamazaki Junko
• Ito Takayuki
• Yamagishi Kazuo

Classifications

• A23L5/00

  Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor · CPC title

• C12P13/14

  Glutamic acid; Glutamine · CPC title

• A23L31/15 Primary

  Extracts · CPC title

• C12Y203/02002

  Gamma-glutamyltransferase (2.3.2.2) · CPC title

• A23L27/24

  prepared by fermentation · CPC title