Pyruvate dehydrogenase variants, a microorganism comprising the same and a method for producing L-amino acid using the same

Patent metadata

Field	Value
Publication number	US-10526586-B2
Application number	US-201615557013-A
Country	US
Kind code	B2
Filing date	Mar 15, 2016
Priority date	Mar 18, 2015
Publication date	Jan 7, 2020
Grant date	Jan 7, 2020

Abstract

The present disclosure relates to a novel pyruvate dehydrogenase variant, a polynucleotide encoding the pyruvate dehydrogenase variant, a microorganism of the genus Corynebacterium producing L-amino acid, which includes the pyruvate dehydrogenase variant, and a method for producing an L-amino acid using the microorganism.

First claim

The invention claimed is:

1. A polypeptide having pyruvate dehydrogenase activity, wherein the polypeptide comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1, and wherein at least one of the amino acids at the positions corresponding to positions 418, 428, 432, 435, and 438 of SEQ ID NO: 1 is substituted with a different amino acid in the amino acid sequence of the polypeptide.

2. The polypeptide of claim 1 , wherein the substitution is selected from the group consisting of a substitution at position 418 from tyrosine to histidine (Y418H), a substitution at position 428 from asparagine to alanine (N428A), a substitution at position 432 from glutamine to glutamic acid (Q432E), a substitution at position 432 from glutamine to alanine (Q432A), a substitution at position 435 from lysine to alanine (K435A), and a substitution at position 438 from leucine to proline (L438P).

3. The polypeptide of claim 1 , wherein the amino acid sequence of the polypeptide is selected from the group consisting of SEQ ID NO: 14, 16, 19, 20, 21, 23, 30, 31, 32, and 33.

4. A polynucleotide comprising a nucleotide sequence encoding the polypeptide of claim 1 .

5. A microorganism of the genus Corynebacterium producing an L-amino acid, wherein the microorganism comprises the polypeptide of claim 1 .

6. The microorganism of claim 5 , wherein the microorganism is Corynebacterium glutamicum.

7. A method for producing an L-amino acid, comprising:
(a) culturing the microorganism of claim 5 in a medium to produce an L-amino acid; and
(b) recovering the L-amino acid from the cultured microorganism or the medium.

8. A microorganism of the genus Corynebacterium producing an L-amino acid, wherein the microorganism comprises the polypeptide of claim 2 .

9. A method for producing an L-amino acid, comprising:
(a) culturing the microorganism of claim 8 in a medium to produce an L-amino acid; and
(b) recovering the L-amino acid from the cultured microorganism or the medium.

10. A microorganism of the genus Corynebacterium producing an L-amino acid, wherein the microorganism comprises the polypeptide of claim 3 .

11. A method for producing an L-amino acid, comprising:
(a) culturing the microorganism of claim 10 in a medium to produce an L-amino acid; and
(b) recovering the L-amino acid from the cultured microorganism or the medium.

Assignees

• Cj Cheiljedang Corp

Inventors

• Huh Lan
• Moon Jun Ok
• Bae Hyun Won
• Kim Hyung Joon
• Song Sung Ki

Classifications

• C12N15/77

  for Corynebacterium; for Brevibacterium · CPC title

• C12N9/0008 Primary

  acting on the aldehyde or oxo group of donors (1.2) · CPC title

• C12P13/08

  Lysine; Diaminopimelic acid; Threonine; Valine · CPC title

• C12P13/04

  Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title

• C12Y102/01051

  Pyruvate dehydrogenase (NADP+) (1.2.1.51) · CPC title