Method of producing sugar liquid

Patent metadata

Field	Value
Publication number	US-10519476-B2
Application number	US-201314401900-A
Country	US
Kind code	B2
Filing date	May 17, 2013
Priority date	May 18, 2012
Publication date	Dec 31, 2019
Grant date	Dec 31, 2019

Abstract

A method of producing a sugar liquid from cellulose-containing biomass includes (1) to (3): (1): subjecting a cellulose-containing biomass to hydrothermal treatment and thereafter separating the treated cellulose-containing biomass into a hydrothermally-treated liquid and a cellulose-containing solid content; (2): adding a filamentous fungus-derived cellulase to the cellulose-containing solid content obtained in (1) to hydrolyze the cellulose and thereafter separating the hydrolysate into a saccharification residue and a sugar liquid; and (3): washing the saccharification residue obtained in (2) with the hydrothermally-treated liquid obtained in (1) to elute the filamentous fungus-derived cellulase adsorbed to the saccharification residue into the hydrothermally-treated liquid and thereafter obtaining a solution component comprising the filamentous fungus-derived cellulase by solid-liquid separation.

First claim

The invention claimed is:

1. A method of producing a sugar liquid from cellulose-containing biomass comprising (1) to (3):
(1): subjecting a cellulose-containing biomass to hydrothermal treatment and thereafter separating the treated cellulose-containing biomass into a hydrothermally-treated liquid and a cellulose-containing solid content;
(2): adding a filamentous fungus-derived cellulase to the cellulose-containing solid content obtained in (1) to hydrolyze the cellulose and thereafter separating the hydrolysate into a saccharification residue and a sugar liquid; and
(3): washing the saccharification residue obtained in the step (2) with the hydrothermally-treated liquid obtained in (1) to elute the filamentous fungus-derived cellulase adsorbed to the saccharification residue into the hydrothermally-treated liquid and thereafter obtaining a solution component comprising the filamentous fungus-derived cellulase by solid-liquid separation,
wherein the hydrothermally-treated liquid used in the step (3) comprises enzymatic saccharification inhibitors.

2. The method according to claim 1 , comprising (4) filtering the solution component obtained in (3) through an ultrafiltration membrane to thereby recover the filamentous fungus-derived cellulase as a retentate and at the same time obtain a sugar liquid as a permeate.

3. The method according to claim 2 , wherein the filamentous fungus-derived cellulase recovered in (4) is reused in the cellulose hydrolysis in (2).

4. The method according to claim 1 , wherein the filamentous fungus-derived cellulase is cellulase derived from Trichoderma.

5. The method according to claim 1 , wherein the hydrothermal treatment of (1) is a treatment at 120 to 240° C.

6. The method according to claim 1 , wherein the hydrothermally-treated liquid used in (3) comprises 1 g/L or more of an inorganic ion, acetic acid and/or furfural in total.

7. The method according to claim 1 , wherein the saccharification residue is washed with a hydrothermally-treated liquid at 30 to 70° C. in (3).

8. The method according to claim 1 , wherein (2) comprises separating the hydrolysate into a saccharification residue and a sugar liquid by membrane separation, and (3) comprises washing the saccharification residue by passing the hydrothermally-treated liquid through the saccharification residue on the surface of the membrane in a vertical direction to obtain a solution component comprising the filamentous fungus-derived cellulase.

9. The method according to claim 8 , wherein the membrane separation is press filtration or membrane separation by a belt filter.

10. A method of producing a chemical substance comprising producing the sugar liquid by the method according to claim 1 and culturing a microorganism capable of producing a chemical substance using the sugar liquid as a fermentation raw material.

Assignees

• Toray Industries

Inventors

• Kurihara Hiroyuki
• Ishizuka Hiroko
• Minamino Atsushi
• Yamada Katsushige

Classifications

• C12M21/18

  Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes · CPC title

• C13K13/002

  Xylose · CPC title

• C12M45/09

  by enzymatic treatment · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12M45/20

  Heating; Cooling (heating or cooling apparatus for laboratory uses B01L7/00) · CPC title