Manufacturing method for sugar solution and device for same

Patent metadata

Field	Value
Publication number	US-9624516-B2
Application number	US-201113634961-A
Country	US
Kind code	B2
Filing date	Mar 14, 2011
Priority date	Mar 15, 2010
Publication date	Apr 18, 2017
Grant date	Apr 18, 2017

Abstract

A method produces a sugar liquid by repeating a sugar liquid production process including (1) to (3): (1) adding a filamentous fungus-derived cellulase to cellulose to perform primary hydrolysis; (2) adding a fresh filamentous fungus-derived cellulase to the hydrolysate in Step (1) to perform secondary hydrolysis; and (3) subjecting the hydrolysate in Step (2) to solid-liquid separation to obtain a sugar liquid, from which a recovered enzyme is obtained; wherein the recovered enzyme obtained in Step (3) is used for Step (1) of the next and later sugar liquid production processes.

First claim

The invention claimed is:

1. A method of producing a sugar liquid by repeating a sugar liquid production process comprising (1) to (3):
(1) adding a filamentous fungus-derived cellulase to cellulose which is a processed product obtained by subjecting a cellulose-containing biomass to alkaline treatment, hydrothermal treatment or dilute sulfuric acid treatment and has not been subjected to enzyme treatment to perform primary hydrolysis;
(2) adding a fresh filamentous fungus-derived cellulase without adding cellulose to a hydrolysate formed in (1) without separating a solid residue in hydrolysate to perform secondary hydrolysis; and
(3) subjecting another hydrolysate formed in (2) to solid-liquid separation to obtain a sugar liquid and recovering enzyme from the sugar liquid;
wherein recovered enzyme obtained in (3) is used for (1) of next and later sugar liquid production processes, and
as said filamentous fungus-derived cellulase in (1) of the sugar liquid production process, an enzyme component recovered from a cellulose hydrolysate produced by a filamentous fungus-derived cellulase is used.

2. The method according to claim 1 , wherein said filamentous fungus-derived cellulase in (1) or (2) comprises a component derived from a culture liquid of a microorganism belonging to the genus Trichoderma.

3. The method according to claim 1 , wherein said recovered enzyme comprises xylanase and/or xylosidase.

4. The method according to claim 1 , wherein said recovered enzyme comprises a water-insoluble filamentous fungus-derived cellulase.

5. The method according to claim 1 , wherein amounts of enzyme added in said primary hydrolysis and said secondary hydrolysis satisfy a following relation: an amount of said recovered enzyme added in (1) > an amount of said fresh enzyme added in (2).

6. The method according to claim 1 , wherein recovery of said filamentous fungus-derived cellulase in (3) is carried out by filtering said sugar liquid through an ultrafiltration membrane and recovering said cellulase from the feed side.

7. The method according to claim 2 , wherein said recovered enzyme comprises xylanase and/or xylosidase.

8. The method according to claim 2 , wherein said recovered enzyme comprises a water-insoluble filamentous fungus-derived cellulase.

9. The method according to claim 3 , wherein said recovered enzyme comprises a water-insoluble filamentous fungus-derived cellulase.

10. The method according to claim 1 , wherein a reaction time of the primary hydrolysis in (1) is 15 minutes to 6 hours.

11. The method according to claim 10 , wherein a reaction time of the secondary hydrolysis in (2) is 6 hours to 72 hours.

Assignees

• Kurihara Hiroyuki
• Minamino Atsushi
• Yamamoto Yuki
• Yamada Katsushige
• Toray Industries

Inventors

• Kurihara Hiroyuki
• Minamino Atsushi
• Yamamoto Yuki
• Yamada Katsushige

Classifications

• C12M29/04

  Filters; Permeable or porous membranes or plates, e.g. dialysis · CPC title

• C13K1/02

  obtained by saccharification of cellulosic materials (manufacture of fodder A23K10/32) · CPC title

• C12M21/18 Primary

  Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes · CPC title

• C12M33/14

  with filters, sieves or membranes · CPC title

• C12P19/14 Primary

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title