Polymer dot compositions and related methods

Patent metadata

Field	Value
Publication number	US-10514381-B2
Application number	US-201414774971-A
Country	US
Kind code	B2
Filing date	Mar 14, 2014
Priority date	Mar 14, 2013
Publication date	Dec 24, 2019
Grant date	Dec 24, 2019

Abstract

Lyophilized chromophoric polymer dot compositions are provided. Also disclosed are methods of making and using the lyophilized compositions, methods of dispersing the lyophilized compositions in aqueous solutions and kits supplying the compositions.

First claim

What is claimed is:

1. A lyophilized composition comprising:
a lyophilization agent that comprises a carbohydrate; and
a plurality of fluorescent nanoparticles, wherein:
each fluorescent nanoparticle in the plurality of fluorescent nanoparticles comprises at least one condensed conjugated, semiconducting polymer; and
the plurality of fluorescent nanoparticles after lyophilization has a quantum yield that is greater than 80% of the quantum yield of the plurality of fluorescent nanoparticles that have not been lyophilized,

wherein the lyophilization agent is present between about 1% w/v and 50% w/v.

2. The lyophilized composition of claim 1 , wherein the carbohydrate comprises a monosaccharide, a disaccharide, an oligosaccharide, a polysaccharide, or any combination thereof.

3. The lyophilized composition of claim 2 , wherein the disaccharide is selected from the group consisting of sucrose, trehalose dihydrate, maltose monohydrate, and lactose monohydrate.

4. The lyophilized composition of claim 1 , wherein the lyophilization agent further comprises an alditol, hydroxypropyl-cyclodextrin, BSA, or any combination thereof.

5. The lyophilized composition of claim 1 , wherein the plurality of fluorescent nanoparticles has an average diameter of less than about 30 nm as measured by dynamic light scattering.

6. The lyophilized composition of claim 1 , wherein the plurality of fluorescent nanoparticles has an average diameter of between 30 nm and 100 nm as measured by dynamic light scattering.

7. The lyophilized composition of claim 1 , wherein the plurality of fluorescent nanoparticles has an average diameter of less than about 100 nm as measured by dynamic light scattering.

8. The lyophilized composition of claim 1 , wherein the plurality of fluorescent nanoparticles after lyophilization comprises a similar or increased quantum yield when dispersed in an aqueous solution as compared to the plurality of fluorescent nanoparticles prior to lyophilization.

9. The lyophilized composition of claim 1 , wherein at least some fluorescent nanoparticles in the plurality of fluorescent nanoparticles are conjugated to a biomolecule.

10. The lyophilized composition of claim 9 , wherein the biomolecule is a protein, a nucleic acid molecule, a lipid, or a peptide.

11. The lyophilized composition of claim 9 , wherein the biomolecule is an antibody or an aptamer.

12. The lyophilized composition of claim 1 , wherein each fluorescent nanoparticle in the plurality of fluorescent nanoparticles comprises a plurality of polymers.

13. The lyophilized composition of claim 1 , wherein the plurality of fluorescent nanoparticles after lyophilization have a similar average particle diameter when dispersed in an aqueous solution as compared to the average particle diameter of the plurality of fluorescent nanoparticles prior to lyophilization.

14. The lyophilized composition of claim 1 , wherein the at least one condensed conjugated, semiconducting polymer is selected from the group consisting of a fluorene polymer, a phenylene vinylene polymer, a phenylene ethynylene polymer, a BODIPY polymer, and any derivative thereof.

15. A kit comprising:
a composition comprising a lyophilized lyophilization agent that comprises a carbohydrate and a plurality of lyophilized fluorescent nanoparticles, wherein:
each lyophilized fluorescent nanoparticle in the plurality comprises at least one condensed conjugated, semiconducting polymer; and
the plurality of lyophilized fluorescent nanoparticles after lyophilization has a quantum yield that is greater than 80% of the quantum yield of the plurality of fluorescent nanoparticles that have not been lyophilized; and

a set of instructions describing combining the composition with an aqueous solution,
wherein the lyophilized lyophilization agent is present between about 1% w/v and 50% w/v.

16. The kit of claim 15 , wherein the lyophilized lyophilization agent further comprises an alditol, hydroxypropyl-cyclodextrin, BSA, or any combination thereof.

17. The kit of claim 15 , wherein at least some fluorescent nanoparticles in the plurality of fluorescent nanoparticles are conjugated to a biomolecule.

18. The kit of claim 15 , wherein the at least one condensed conjugated, semiconducting polymer is selected from the group consisting of a fluorene polymer, a phenylene vinylene polymer, a phenylene ethynylene polymer, a BODIPY polymer, and any derivative thereof.

19. The lyophilized composition of claim 1 , wherein the at least one condensed conjugated, semiconducting polymer comprises a narrow-band emissive unit.

20. The lyophilized composition of claim 19 , wherein the narrow-band emissive unit is covalently attached to the at least one condensed conjugated, semiconducting polymer.

21. The lyophilized composition of claim 19 , wherein the narrow-band emissive unit comprises a BODIPY monomer, a squaraine monomer, a metal complex, a porphyrin, a phthalocyanine, a lanthanide complex, a perylene, a cyanine, a rhodamine, a coumarin, a xanthene, or any derivative thereof.

22. The lyophilized composition of claim 14 , wherein the at least one condensed conjugated, semiconducting polymer is a copolymer.

Assignees

• Univ Washington Through Its Center For Commercialization

Inventors

• Chiu Daniel T
• Sun Wei
• Yu Jiangbo
• Wu Changfeng
• Ye Fangmao

Classifications

• C09K11/025

  non-luminescent particle coatings or suspension media · CPC title

• G01N33/587

  Nanoparticles · CPC title

• G01N33/533

  with fluorescent label · CPC title

• C08G2261/18

  conjugated · CPC title

• G01N33/54393 Primary

  Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding · CPC title