Polypeptide having a polyester degrading activity and uses thereof

Patent metadata

Field	Value
Publication number	US-10508269-B2
Application number	US-201615556655-A
Country	US
Kind code	B2
Filing date	Mar 11, 2016
Priority date	Mar 13, 2015
Publication date	Dec 17, 2019
Grant date	Dec 17, 2019

Abstract

The present invention relates to a new isolated polypeptide comprising an amino acid sequence having at least 75%, 80%, 85%, 90%, 95%, 99% or 100% identity to the full length amino acid sequence set forth in SEQ ID NO:1, and having a polyester degrading activity, and uses thereof.

First claim

The invention claimed is:

1. A nucleic acid operably linked to a heterologous promoter, said nucleic acid encoding a polypeptide having a polyester degrading activity and having at least 90% identity to the full length amino acid sequence of SEQ ID NO: 1.

2. An expression cassette comprising the nucleic acid of claim 1 .

3. A vector comprising the nucleic acid of claim 1 .

4. A recombinant cell containing a nucleic acid of claim 1 .

5. A method of producing a polypeptide, comprising:
(i) culturing a recombinant cell of claim 4 to express the polypeptide, said polypeptide having a polyester degrading activity and having at least 90% identity to the full length amino acid sequence of SEQ ID NO: 1; and
(ii) recovering the expressed polypeptide from the culture supernatant or the cells.

6. A method for degrading a polyester containing material, comprising the step of exposing the polyester containing material to a polypeptide having a polyester degrading activity, said polypeptide comprising an amino acid sequence having at least 90% sequence identity to the full length amino acid sequence of SEQ ID NO: 1.

7. The method of claim 6 , wherein at least one polyester of the polyester containing material is depolymerized up to monomers and/or oligomers.

8. The method of claim 6 , comprising a pretreatment step of the polyester containing material to submit the polyester containing material to at least one modification selected from mechanical modification, physical modification, chemical modification, biological modification.

9. The method of claim 6 , comprising a subsequent step of b) recovering the resulting monomers and/or oligomers.

10. A plastic compound containing a polypeptide having a polyester degrading activity and comprising at least 90% sequence identity to the full length amino acid sequence of SEQ ID NO: 1, and at least one polyester.

11. A process for producing a plastic compound, wherein at least one polyester and a polypeptide having a polyester degrading activity, said polypeptide comprising an amino acid sequence comprising at least 90% sequence identity to the full length amino acid sequence of SEQ ID NO: 1 are mixed at a temperature at which the polyester is in a partially or totally molten state.

12. The plastic compound of claim 10 , wherein the polypeptide further comprises, at the C-terminal end or at the N-terminal end of said amino acid sequence having at least 90% sequence identity to the full length amino acid sequence of SEQ ID NO: 1, an amino acid sequence, that has at least 75% sequence identity to the full length amino acid sequence of SEQ ID NO: 3.

13. The process for preparing a plastic compound of claim 11 , wherein the step of mixing a polyester with the polypeptide is performed at a temperature at which the polyester is in a partially or totally molten state.

14. The process for preparing a plastic compound of claim 13 , wherein the step of mixing is performed by extrusion.

15. The process for preparing a plastic compound of claim 11 , wherein the polyester is selected from the group consisting of PLA, PBS and PHAs.

16. The process for preparing a plastic compound of claim 15 , wherein the polyester is PLA and the step of mixing is performed at a temperature at which PLA is in a partially or totally molten state.

17. The method of claim 6 , wherein the polyester is PLA and the polypeptide has a PLA degrading activity.

18. The method of claim 6 , wherein the polypeptide further comprises, at the C-terminal end or at the N-terminal end of said amino acid sequence having at least 90% sequence identity to the full length amino acid sequence of SEQ ID NO: 1, an amino acid sequence that has at least 75% sequence identity to the full length amino acid sequence of SEQ ID NO: 3.

19. The method of claim 6 , wherein the polypeptide having a polyester degrading activity comprises an amino acid sequence having at least 95% sequence identity to the full length amino acid sequence of SEQ ID NO: 1.

20. The plastic compound of claim 10 , wherein the polypeptide having a polyester degrading activity comprises an amino acid sequence having at least 95% sequence identity to the full length amino acid sequence of SEQ ID NO: 1.

21. The process of claim 11 , wherein the polypeptide having a polyester degrading activity comprises an amino acid sequence having at least 95% sequence identity to the full length amino acid sequence of SEQ ID NO: 1.

Assignees

• Carbios
• Centre Nat Rech Scient
• Univ Poitiers

Inventors

• Li Luen-Luen
• Ferreira Thierry
• Aucher Willy
• Colas Steven
• Kadri Linette Lionelle

Classifications

• C12P7/18

  polyhydric · CPC title

• C12P7/42

  Hydroxy-carboxylic acids · CPC title

• C08J11/105

  by treatment with enzymes · CPC title

• C12N15/00

  Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor (mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00) · CPC title

• C12P7/625

  Polyesters of hydroxy carboxylic acids · CPC title