Pasteurisation process for microbial cells and microbial oil

Patent metadata

Field	Value
Publication number	US-10493174-B2
Application number	US-201615248065-A
Country	US
Kind code	B2
Filing date	Aug 26, 2016
Priority date	Jun 19, 2002
Publication date	Dec 3, 2019
Grant date	Dec 3, 2019

Abstract

An improved pasteurisation protocol for pasteurising microbial cells is disclosed. The protocol has three stages, a first heating stage, a second plateau stage at which the cells are held at a (maximum and) constant temperature, and a third cooling stage. Both the heating and the cooling stages are rapid, with the temperature of the cells passing through 40 to 80° C. in no more than 30 minutes in the heating stage. The heating rate is at least 0.5° C./minute and during cooling is at least −0.5° C./minute. The plateau maximum temperature is from 70 to 85° C. By plotting the pasteurisation protocol on a time (t, minutes) versus temperature (T, ° C.) graph, one obtains a trapezium having an area less than 13,000° C. minute. Not only does this result in a smaller energy input (and so a reduction in costs), but a better quality (and less oxidized) oil results having a peroxide value (POV) of less than 1.5 and an anisidine value (AnV) of less than 1.0.

First claim

The invention claimed is:

1. An isolated or extracted microbial oil comprising at least 40% of a desired polyunsaturated fatty acid (PUFA); a peroxide value (POV) of less than 3.0; and an anisidine value (AnV) of no more than 25.

2. The microbial oil of claim 1 wherein the PUFA comprises a C 20 or C 22 ω-3 or ω-6 fatty acid.

3. An oil according to claim 1 or claim 2 , wherein the PUFA is arachidonic acid (ARA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA).

4. An oil according to claim 1 , which has a triglyceride content of at least 90%.

5. An oil according to claim 1 , wherein the oil comprises at least 45% of the desired PUFA.

6. An oil according to claim 1 , wherein the oil has an AnV of no more than 20.

7. An oil according to claim 1 , wherein the oil has an AnV of no more than 15.

8. An oil according to claim 1 , wherein the oil has an AnV of from 5 to 25.

9. An oil according to claim 1 , wherein the oil has an AnV of from 5 to 20.

10. An oil according to claim 1 , wherein the oil has an AnV of from 5 to 15.

11. An oil according to claim 1 , wherein the oil has an AnV of from 10 to 20.

12. An oil according to claim 1 , wherein the oil is extracted or isolated from cells which are fungi, bacteria or algae.

13. An oil according to claim 12 , wherein the cells are of the genus Mortierella.

14. An oil according to claim 13 , wherein the cells are of the species Mortierella alpine.

15. An oil according to claim 12 , wherein the cells are of the genus Crypthecodinium.

16. An oil according to claim 15 , wherein the cells are of the species Crypthecodinium cohnii.

17. An oil according to claim 12 , wherein the cells are Thraustochytrium cells.

18. The oil of claim 1 , wherein the oil is a crude or unrefined oil.

19. The oil according to claim 18 which has been subjected to one or more refining steps.

20. The oil according to claim 19 , wherein the refining steps include acid treatment or degumming, alkali treatment or free fatty acid removal, bleaching or pigment removal, filtration, winterization, deodorizing and/or polishing.

21. The oil according to claim 19 or 20 , which comprises adding the refined oil to human foods or animal foodstuffs.

22. The oil according to claim 19 or 20 , which comprises adding the refined oil to infant formula.

Assignees

• Dsm Ip Assets Bv

Inventors

• Schaap Albert
• Verkoeijen Daniel

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P25/00

  Drugs for disorders of the nervous system · CPC title

• A23L33/12

  Fatty acids or derivatives thereof · CPC title

• C11B1/10

  by extracting · CPC title

• A61K8/67

  Vitamins · CPC title