Pasteurisation process for microbial cells and microbial oil

Patent metadata

Field	Value
Publication number	US-9457108-B2
Application number	US-201414311461-A
Country	US
Kind code	B2
Filing date	Jun 23, 2014
Priority date	Jun 19, 2002
Publication date	Oct 4, 2016
Grant date	Oct 4, 2016

Abstract

A pasteurization protocol for pasteurizing microbial cells is disclosed. The protocol has a heating stage, a plateau stage at which the cells are held at a (maximum and) constant temperature, and a cooling stage. The heating and cooling stages are rapid, the temperature of the cells passing through 40 to 80° C. in no more than 30 minutes in the heating stage. The heating rate is at least 0.5° C./minute and during cooling is at least −0.5° C./minute. The plateau maximum temperature is from 70 to 85° C. By plotting the pasteurization protocol on a time (t, minutes) versus temperature (T, ° C.), one obtains a trapezium having an area less than 13,000° C. minute. This results in a smaller energy input (so a reduction in costs) and a better quality oil having a peroxide value (POV) of less than 1.5 and an anisidine value (AnV) of less than 1.0.

First claim

The invention claimed is:

1. A microbial oil comprising docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and mixtures thereof having a triglyceride content of at least 90%; a peroxide value (POV) of less than 1.5, or optionally less than 1.0; and/or an anisidine value (AnV) of less than 15, or optionally less than 12.

2. The microbial oil of claim 1 wherein the oil comprises at least 40% DHA, EPA, and mixtures thereof and/or the oil is a crude or unrefined oil.

3. The microbial oil of claim 1 , wherein the DHA, EPA, and mixtures thereof are produced from microbial cells, wherein the microbial cells are selected from Cryptochodinium, Thraustochytrium , and mixtures thereof.

4. The microbial oil of claim 3 , wherein the microbial cells are Cryptochodinium .

5. The microbial oil of claim 4 , wherein the microbial cells are Cryptochodinium Cohnii .

6. The microbial oil of claim 3 , wherein the DHA, EPA and mixtures thereof are produced from microbial cells, wherein the microbial cells are Thraustochytrium .

7. A foodstuff comprising the microbial oil of claim 2 .

8. The foodstuff of claim 7 , which is an animal feed or a marine feed.

9. The microbial oil of claim 2 , wherein the microbial oil is subjected to one or more refining steps to provide a refined oil, wherein said one or more refining steps are selected from the group consisting of acid treatment or degumming, alkali treatment or free fatty acid removal, bleaching or pigment removal, filtration, winterization, deodorizing and polishing.

10. A foodstuff comprising the refined oil of claim 9 .

11. The foodstuff of claim 10 , which is an infant formula.

12. A microbial oil comprising DHA, EPA, and mixtures thereof having a triglyceride content of at least 50%; a peroxide value (POV) of less than 1.5, or optionally less than 1.0; and/or an anisidine value (AnV) of less than 15, or optionally less than 12.

13. The microbial oil of claim 12 wherein the oil comprises at least 40% DHA, EPA, and mixtures thereof and/or the oil is a crude or unrefined oil.

14. A foodstuff comprising the microbial oil of claim 13 .

15. The foodstuff of claim 14 , which is an animal feed or a marine feed.

16. The microbial oil of claim 13 , wherein the microbial oil is subjected to one or more refining steps to provide a refined oil, wherein said one or more refining steps are selected from the group consisting of acid treatment or degumming, alkali treatment or free fatty acid removal, bleaching or pigment removal, filtration, winterization, deodorizing and polishing.

17. A foodstuff comprising the refined oil of claim 16 .

18. The foodstuff of claim 17 , which is an infant formula.

19. The microbial oil of claim 12 , wherein the DHA, EPA and mixtures thereof are produced from microbial cells, wherein the microbial cells are selected from Cryptochodinium, Thraustochytrium , and mixtures thereof.

20. The microbial oil of claim 19 , wherein the microbial cells are Cryptochodinium .

21. The microbial oil of claim 20 , wherein the microbial cells are Cryptochodinium Cohnii .

22. The microbial oil of claim 12 , wherein the DHA, EPA and mixtures thereof are produced from microbial cells, wherein the microbial cells are Thraustochytrium .

23. A foodstuff comprising the microbial oil of claim 1 .

24. The foodstuff of claim 23 , which is an animal feed or a marine feed.

25. The microbial oil of claim 12 , wherein the microbial oil is subjected to one or more refining steps to provide a refined oil, wherein said one or more refining steps are selected from the group consisting of acid treatment or degumming, alkali treatment or free fatty acid removal, bleaching or pigment removal, filtration, winterization, deodorizing and polishing.

26. A foodstuff comprising the refined oil of claim 25 .

27. The foodstuff of claim 26 , which is an infant formula.

Assignees

• Dsm Ip Assets Bv

Inventors

• Schaap Albert
• Verkoeijen Daniel

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P25/00

  Drugs for disorders of the nervous system · CPC title

• A61K8/9706

  Algae · CPC title

• A61K8/9728

  Fungi, e.g. yeasts · CPC title

• A61K8/925

  of animal origin · CPC title