Preparative separation chromatograph

Patent metadata

Field	Value
Publication number	US-10473633-B2
Application number	US-201615088280-A
Country	US
Kind code	B2
Filing date	Apr 1, 2016
Priority date	Apr 3, 2015
Publication date	Nov 12, 2019
Grant date	Nov 12, 2019

Abstract

Provided is a preparative separation chromatograph including: a chromatograph 10 having a detector 15; a fraction collector 20; a controller 34 for commanding the fraction collector to initiate a component-collecting operation with reference to a point when the rate of change in an output signal from the detector 15 exceeds a positive reference value and to discontinue the operation with reference to a point when the absolute value of the rate of change becomes smaller than that of a negative reference value after the rate of change turns negative; a storage section 31 for storing sampling-rate information which relates the value of the output signal to the time interval at which the controller determines the rate of change; and a sampling rate determiner 33 for determining the time interval for calculating the rate of change based on the sampling-rate information and actual values of the output signal.

First claim

The invention claimed is:

1. A preparative separation chromatograph system, comprising:
a) a chromatograph having a column configured to temporally separate one or a plurality of target components contained in a sample and a detector configured to detect each target component eluted from the column;
b) a fraction collector configured to collect a target component exiting from the detector;
c) a controller configured to execute controller-executable instructions to:
store, in a storage section, sampling-rate information which relates a plurality of threshold values of an output signal from the detector to a plurality of sampling rates, the sampling-rate information being prepared so that a higher sampling rate is set for lower levels of the output signal, while a lower sampling rate is set for higher levels of the output signal;
acquire a value of the output signal from the detector;
determine a sampling rate corresponding to the acquired value of the output signal with reference to the stored sampling-rate information;
determine a rate of change in the output signal in the determined sampling rate;
command the fraction collector to initiate a collecting operation for the target component with reference to a point in time where the rate of change becomes greater than a positive predetermined reference value; and
command the fraction collector to discontinue the collecting operation for the target component with reference to a point in time where an absolute value of the rate of change becomes smaller than an absolute value of a negative predetermined reference value after the rate of change turns negative.

2. A method for collecting a target component using a preparative separation chromatograph, the method comprising:
storing sampling-rate information which relates a plurality of threshold values of an output signal from a detector to a plurality of sampling rates, the sampling-rate information being prepared so that a higher sampling rate is set for lower levels of the output signal, while a lower sampling rate is set for higher levels of the output signal;
acquiring a value of the output signal from the detector;
determining a sampling rate corresponding to the acquired value of the output signal with reference to the stored sampling-rate information;
determining a rate of change in the output signal in the determined sampling rate;
initiating a collecting operation for a target component with reference to a point in time where the rate of change in a detection signal of one or a plurality of target components temporally separated by a column in a chromatograph becomes greater than a positive predetermined reference value; and
discontinuing the collecting operation for the target component with reference to a point in time where an absolute value of the rate of change becomes smaller than an absolute value of a negative predetermined reference value after the rate of change turns negative.

Assignees

• Shimadzu Corp

Inventors

• Okoba Tsutomu
• Iriki Takayuki

Classifications

• B01D15/247

  Fraction collectors · CPC title

• B01D15/24

  relating to the treatment of the fractions to be distributed · CPC title

• G01N30/80 Primary

  Fraction collectors · CPC title

• G01N30/34

  of fluid composition, e.g. gradient (G01N30/36 takes precedence) · CPC title

• G01N30/8658

  Optimising operation parameters · CPC title