Preparative separation chromatograph

Patent metadata

Field	Value
Publication number	US-2016290976-A1
Application number	US-201615088280-A
Country	US
Kind code	A1
Filing date	Apr 1, 2016
Priority date	Apr 3, 2015
Publication date	Oct 6, 2016
Grant date	—

Abstract

Provided is a preparative separation chromatograph including: a chromatograph 10 having a detector 15; a fraction collector 20; a controller 34 for commanding the fraction collector to initiate a component-collecting operation with reference to a point when the rate of change in an output signal from the detector 15 exceeds a positive reference value and to discontinue the operation with reference to a point when the absolute value of the rate of change becomes smaller than that of a negative reference value after the rate of change turns negative; a storage section 31 for storing sampling-rate information which relates the value of the output signal to the time interval at which the controller determines the rate of change; and a sampling rate determiner 33 for determining the time interval for calculating the rate of change based on the sampling-rate information and actual values of the output signal.

First claim

1 . A preparative separation chromatograph, comprising:
a) a chromatograph having a column for temporally separating one or a plurality of target components contained in a sample and a detector for detecting each target component eluted from the column; b) a fraction collector for collecting a target component exiting from the detector; c) a controller for determining a rate of change in an output signal from the detector based on the output signal, for commanding the fraction collector to initiate a collecting operation for the target component with reference to a point in time where the rate of change becomes greater than a positive predetermined reference value, and for commanding the fraction collector to discontinue the collecting operation for the target component with reference to a point in time where an absolute value of the rate of change becomes smaller than an absolute value of a negative predetermined reference value after the rate of change turns negative; d) a storage section for storing sampling-rate information in which a value of the output signal from the detector is related to a time interval at which the controller determines the rate of change in the output signal; and e) a sampling rate determiner for determining the time interval at which the controller determines the rate of change in the output signal, based on the sampling-rate information and the value of the output signal acquired from the detector by the controller.

2 . A method for collecting a target component using a preparative separation chromatograph configured to initiate a collecting operation for a target component with reference to a point in time where a rate of change in a detection signal of one or a plurality of target components temporally separated by a column in a chromatograph becomes greater than a positive predetermined reference value and to discontinue the collecting operation for the target component with reference to a point in time where an absolute value of the rate of change becomes smaller than an absolute value of a negative predetermined reference value after the rate of change turns negative, wherein:
the rate of change in the detection signal is determined at time intervals depending on a magnitude of a value of the detection signal.

Assignees

• Shimadzu Corp

Inventors

• Okoba Tsutomu
• Iriki Takayuki

Classifications

• B01D15/24

  relating to the treatment of the fractions to be distributed · CPC title

• G01N30/34

  of fluid composition, e.g. gradient (G01N30/36 takes precedence) · CPC title

• B01D15/247

  Fraction collectors · CPC title

• G01N30/82

  Automatic means therefor · CPC title

• G01N30/8651

  Recording, data aquisition, archiving and storage · CPC title