Lignin-degrading methods
US-9796993-B2 · Oct 24, 2017 · US
Recombinant protein, recombinant microorganism, and method of degrading macromolecular substance
US10465210B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10465210-B2 |
| Application number | US-201715724979-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 4, 2017 |
| Priority date | Oct 4, 2016 |
| Publication date | Nov 5, 2019 |
| Grant date | Nov 5, 2019 |
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- Title
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Abstract
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Provided are a recombinant protein, a gene encoding the recombinant protein, a recombinant microorganism including the gene, and a method of degrading a macromolecular substance using the recombinant microorganism or the recombinant protein.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of degrading a polyalkylene compound, the method comprising: contacting the polyalkylene compound with a recombinant microorganism, a lysate thereof, or a fraction of the lysate thereof, wherein the recombinant microorganism expresses a recombinant protein comprising the amino acid sequence of SEQ ID NO:1, and wherein the polyalkylene compound has an alkylene repeat unit of 1 to 10 carbon atoms. 2. The method of claim 1 , wherein the contacting is performed in a sealed container. 3. The method of claim 1 , wherein the recombinant microorganism expresses the recombinant protein at an increased level relative to a parent strain. 4. The method of claim 1 , wherein the recombinant protein catalyzes oxidation or conversion of a carbon-hydrogen or carbon-carbon bond to a carbon-oxygen bond in the polyalkylene compound. 5. The method of claim 1 , wherein the recombinant microorganism comprises an increased copy number of a gene that encodes the recombinant protein relative to a parent strain. 6. The method of claim 5 , wherein the gene comprises the nucleotide sequence of SEQ ID NO:8. 7. The method of claim 1 , wherein the recombinant microorganism belongs to the genus Escherichia , the genus Xanthomonas , genus Xanthobacter , the genus Salmonella , the genus Pseudomonas , the genus Corynebacterium , the genus Bacillus , or the genus Saccharomyces. 8. The method of claim 1 , wherein the polyalkylene compound is polyethylene.
Assignees
Inventors
Classifications
acyclic {(C12P5/007 takes precedence)} · CPC title
Miscellaneous (1.14.99) · CPC title
Nitronate monooxygenase (1.13.12.16) · CPC title
- C12N9/0069Primary
acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13) · CPC title
Alkanesulfonate monooxygenase (1.14.14.5) · CPC title
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Frequently asked questions
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- What does patent US10465210B2 cover?
- Provided are a recombinant protein, a gene encoding the recombinant protein, a recombinant microorganism including the gene, and a method of degrading a macromolecular substance using the recombinant microorganism or the recombinant protein.
- Who is the assignee on this patent?
- Samsung Electronics Co Ltd
- What technology area does this patent fall under?
- Primary CPC classification C12N9/0069. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 05 2019 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).