Method for inhibiting coloring of a syrupy sweetener comprising a non-reducing oligosaccharide having a beta-fructofranosidic linkage and a reducing saccharide, and use thereof
US-9215887-B2 · Dec 22, 2015 · US
Lignin-degrading methods
US9796993B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9796993-B2 |
| Application number | US-201214364448-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 12, 2012 |
| Priority date | Dec 12, 2011 |
| Publication date | Oct 24, 2017 |
| Grant date | Oct 24, 2017 |
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Abstract
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A method involving degrading materials that include lignin generally includes contacting a lignin sample with a flavin-dependent monooxygenase and contacting the lignin sample with an auxiliary compound.
First claim
Opening claim text (preview).
What is claimed is: 1. A method comprising: contacting a lignin sample with an isolated flavin-dependent monooxygenase and forming at least partially degraded lignin; and contacting the lignin sample with an auxiliary compound that disrupts noncovalent interaction between the at least partially degraded lignin and the flavin-dependent monooxygenase. 2. The method of claim 1 wherein the lignin sample comprises native lignin. 3. The method of claim 1 wherein the isolated flavin-dependent monooxygenase is expressed in the secretome of a fungus or expressed in a bacterium. 4. The method of claim 3 wherein the fungus is a white-rot fungus or a brown-rot fungus. 5. The method of claim 4 wherein the flavin-dependent monooxygenase is salicylate hydroxylase, salicylate monooxygenase, 4-hydroxybenzoate monooxygenase, 4-hydroxyphenylacetate monooxygenase, melilotate monooxygenase, imidazoleacetate monooxygenase, orcinol monooxygenase, phenol monooxygenase, kynurenine monooxygenase, 2,6-dihydroxypyridine monooxygenase, 4-hydroxyphenylacetate monooxygenase, taxifolin monooxygenase, 2,4-dichlorophenol monooxygenase, 3-hydroxybenzoate monooxygenase, 4-aminobenzoate monooxygenase, anthranilate monooxygenase, anhydrotetracycline monooxygenase, anthraniloyl-CoA monooxygenase, 2-hydroxybiphenyl monooxygenase, pentachlorophenol monooxygenase, benzoyl-CoA monooxygenase, 3-hydroxyphenylacetate monooxygenase, 4-hydroxybenzoate monooxygenase, 4-methyl-5-nitrocatechol monooxygenase, 4-aminobenzoate monooxygenase, or 2-octaprenyl-6-methoxyphenol monooxygenase. 6. The method of claim 1 wherein the lignin sample is contacted with the flavin-dependent monooxygenase and the auxiliary compound simultaneously. 7. The method of claim 1 wherein the lignin sample is contacted with the flavin-dependent monooxygenase before it is contacted with the auxiliary compound. 8. The method of claim 1 wherein the lignin sample is a plant biomass that further comprises cellulose or hemicellulose, and the auxiliary compound comprises a cellulase or a hemicellulase, and said cellulase or hemicellulase is provided in an amount sufficient to dissociate uncleaved lignin components or lignin macromolecules not degraded by the flavin-dependent monooxygenase from the at least partially degraded lignin.
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Classifications
produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title
- C12P19/00Primary
Preparation of compounds containing saccharide radicals (ketoaldonic acids C12P7/58) · CPC title
- C12P19/02Primary
Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title
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- What does patent US9796993B2 cover?
- A method involving degrading materials that include lignin generally includes contacting a lignin sample with a flavin-dependent monooxygenase and contacting the lignin sample with an auxiliary compound.
- Who is the assignee on this patent?
- Univ Minnesota
- What technology area does this patent fall under?
- Primary CPC classification C12P19/00. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Oct 24 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).