Recombinant microorganism including genetic modification that increases pyruvate, phosphate dikinase activity and use thereof

Patent metadata

Field	Value
Publication number	US-10465175-B2
Application number	US-201715794926-A
Country	US
Kind code	B2
Filing date	Oct 26, 2017
Priority date	Oct 26, 2016
Publication date	Nov 5, 2019
Grant date	Nov 5, 2019

Abstract

Provided are a recombinant microorganism including a genetic modification that increases a pyruvate, phosphate dikinase activity, a method of producing cellulose using the same, and a method of producing a microorganism having enhanced cellulose productivity.

First claim

What is claimed is:

1. A recombinant microorganism comprising
a genetic modification that increases pyruvate, phosphate dikinase (PPDK) enzyme activity as compared with a microorganism without the genetic modification, wherein the PDDK enzyme belongs to EC 2.7.9.1,
and a genetic modification that decreases membrane-bound glucose dehydrogenase (GDH) activity as compared with a microorganism not comprising the genetic modification;
wherein the microorganism is Clostridium, Komagataeibacter, Propionibacterium, Acetobacter, Agrobacterium , or Escherichia.

2. The recombinant microorganism of claim 1 , wherein the genetic modification that increases PDDK activity is a genetic modification that increases the copy number of a gene encoding the PPDK.

3. The recombinant microorganism of claim 2 , wherein the PPDK gene is from Clostridium, Komagataeibacter, Propionibacterium, Acetobacter, Agrobacterium , or Escherichia.

4. The recombinant microorganism of claim 1 , wherein PPDK is a polypeptide having 95% or higher sequence identity to any of SEQ ID NOs: 1 to 4.

5. The recombinant microorganism of claim 2 , wherein the PPDK gene has 95% or higher sequence identity to any of SEQ ID NOs: 5 to 8.

6. The recombinant microorganism of claim 1 , wherein the genetic modification that decreases membrane-bound glucose dehydrogenase activity is a genetic modification that inactivates or disrupts a gene encoding the membrane-bound glucose dehydrogenase.

7. A method of producing cellulose, the method comprising culturing a recombinant microorganism of claim 1 in a medium; and separating cellulose from the culture.

8. The method of claim 7 , wherein the genetic modification increases the copy number of a gene encoding the PPDK.

9. The method of claim 7 , wherein the medium comprises 1 to 15 mM Mg′.

10. The method of claim 7 , wherein the PPDK is a polypeptide having 95% or higher sequence identity to SEQ ID NO: 1.

11. The method of claim 8 , wherein the PPDK gene has 95% or higher sequence identity to SEQ ID NO: 2.

12. The recombinant microorganism of claim 1 , wherein the genetic modification that increases PDDK activity is a copy number increase in an exogenous gene encoding the PPDK.

13. The recombinant microorganism of claim 1 , wherein the genetic modification that increases PDDK activity is a copy number increase in a heterologous gene encoding the PPDK.

14. The recombinant microorganism of claim 1 , wherein the recombinant microorganism comprises a heterologous gene encoding PDDK, and a genetic modification that inactivates or disrupts a gene encoding the membrane-bound glucose dehydrogenase.

Assignees

• Samsung Electronics Co Ltd

Inventors

• Rhee Hongsoon
• Kang Jinkyu
• Park Jinhwan
• Yang Dongsik

Classifications

• C12N9/1294 Primary

  Phosphotransferases with paired acceptors (2.7.9) · CPC title

• C12Y207/09001

  Pyruvate, phosphate dikinase (2.7.9.1) · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C12P19/04

  Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds · CPC title

• C12Y101/01047

  Glucose 1-dehydrogenase (1.1.1.47) · CPC title