Methods and compositions for extending shelf life of plant products
US-2017114418-A1 · Apr 27, 2017 · US
US9260720B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9260720-B2 |
| Application number | US-201013203686-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 25, 2010 |
| Priority date | Feb 27, 2009 |
| Publication date | Feb 16, 2016 |
| Grant date | Feb 16, 2016 |
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The invention provides genetic constructs which comprise a senescence-specific promoter operably linked to at least one coding sequence, which encodes at least one polypeptide having phosphoenolpyruvate carboxykinase (PCK) activity and/or pyruvate orthophosphate dikinase (PPDK) activity. The constructs have the ability to cause, in transgenic plants, remobilisation of nitrogen during leaf senescence, such that nitrogen can be transported from the leaves to other regions of the plants. The invention provides plant cells and plants transformed with such constructs, methods of producing transgenic plants, and methods of increasing the rate of nitrogen remobilisation and growth rate in senescent plants. The invention also provides harvested plant leaves, such as tobacco leaves, that have been transformed with the genetic constructs, and to smoking articles comprising such harvested plant leaves.
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The invention claimed is: 1. A genetic construct comprising a promoter operably linked to at least one coding sequence, which encodes at least one polypeptide having cytosolic pyruvate orthophosphate dikinase (PPDK) activity, wherein the promoter is a senescence-specific promoter, and wherein the coding sequence, which encodes the polypeptide having cytosolic PPDK activity, comprises a nucleic acid sequence as set out in SEQ ID No. 20, SEQ ID No. 21, or a functional variant or…
Human Necessities · mapped topic
Human Necessities · mapped topic
Chemistry & Metallurgy · mapped topic
Human Necessities · mapped topic
Chemistry & Metallurgy · mapped topic
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