Protein arrays and methods of using and making the same

Patent metadata

Field	Value
Publication number	US-10457935-B2
Application number	US-201113884446-A
Country	US
Kind code	B2
Filing date	Nov 10, 2011
Priority date	Nov 12, 2010
Publication date	Oct 29, 2019
Grant date	Oct 29, 2019

Abstract

Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids.

First claim

What is claimed is:

1. A method for preparing a protein array having a plurality of proteins, the method comprising:
(a) providing a plurality of nucleic acids each having a predefined sequence and each encoding a protein of interest, wherein the plurality of nucleic acids are immobilized on discrete features of a surface; and
(b) expressing in vitro a plurality of proteins of interest from the plurality of nucleic acids, wherein the plurality of proteins are each present in solution at the discrete features on the surface;
wherein each of the plurality of proteins of interest is expressed in a self-contained volume having a size of 1 picoliter to 1 nanoliter.

2. The method of claim 1 , further comprising (c) measuring an activity of each of the plurality of proteins that are present in solution.

3. The method of claim 1 , wherein the plurality of nucleic acids are synthesized on a solid surface.

4. The method of claim 1 , wherein each of the plurality of nucleic acids comprises a regulatory genetic sequence.

5. The method of claim 1 , wherein each of the plurality of proteins of interest is expressed in vitro in a well of a micro-well plate.

6. The method of claim 1 , wherein each of the plurality of proteins of interest is expressed in vitro at a different feature of a solid surface.

Assignees

• Jacobson Joseph
• Hudson Michael E
• Chu Larry Li Yang
• Gen9 Inc

Inventors

• Jacobson Joseph
• Hudson Michael E
• Chu Larry Li-Yang

Classifications

• B01J2219/00509

  Microcolumns · CPC title

• B01J2219/00378

  Piezoelectric or ink jet dispensers · CPC title

• B01J2219/00621

  by physical means, e.g. trenches, raised areas · CPC title

• B01J2219/00722

  Nucleotides · CPC title

• C12N15/1086 Primary

  Preparation or screening of expression libraries, e.g. reporter assays · CPC title