Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis

Patent metadata

Field	Value
Publication number	US-9150853-B2
Application number	US-201313799039-A
Country	US
Kind code	B2
Filing date	Mar 13, 2013
Priority date	Mar 21, 2012
Publication date	Oct 6, 2015
Grant date	Oct 6, 2015

Abstract

Disclosed are methods, compositions and devices for screening a protein library for proteins having a desired activity, such as capable of catalyzing the formation of a bond between two reactants. In an exemplary embodiments, a plurality of proteins are expressed in vitro from a plurality of nucleic acids, the plurality of proteins are exposed with two single stranded oligonucleotides having complementary sequences, each oligonucleotide having a reactant and a fluorophore, the fluorescence of the protein-reactant-oligonucleotide-fluorophore complexes is detected and the complexes showing detectable fluorescence energy transfer are isolated, thereby isolating proteins having the desired enzymatic activity.

First claim

What is claimed is:

1. A method for screening a protein library for a protein having a desired activity, the method comprising:
a) providing a solid surface having a plurality of distinct features, each distinct feature comprising a plurality of single-stranded anchor oligonucleotides hybridized to a plurality of double-stranded nucleic acids, wherein the plurality of double-stranded nucleic acids encode a plurality of proteins;
b) hybridizing a single-stranded oligonucleotide h…

Assignees

• Gen9 Inc

Inventors

• Hudson Michael E
• Jacobson Joseph

Classifications

• C12N15/1034 Primary

  Chemistry & Metallurgy · mapped topic

• C12N15/1086 Primary

  Chemistry & Metallurgy · mapped topic

• C12N15/1037

  Chemistry & Metallurgy · mapped topic